The ImmunoStar VMAT1 antiserum was quality control tested using standard immunohistochemical methods in rat adrenal medulla using biotin/avidin-HRP techniques; the antiserum shows no reactivity in rat CNS.
Specificity of the antiserum was demonstrated by soluble pre-adsorption and Western blot. Tissue staining is completely eliminated by pretreatment of the diluted antibody with an excess of rat VMAT1 peptide residues 502-521.
Western blot analysis of immunoprecipitated rat adrenal homogenates demonstrates a dense immunoreactive band of approximately 55 kD and a minor band of approximately 75 kD.
Photo Description: IHC image of VMAT1 staining in the rat adrenal medulla. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for frozen sectioning. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
Quantity / Volume: 100 µL
State: Lyophilized Whole Serum
Species Reactivity: Rat
Availability: In Stock
Alternate Names: Chromaffin granule amine transporter; Solute carrier family 18 member 1; VAT1; VMAT-1; Vesicular amine transporter 1; Slc18a1. Cgat, Vmat1,Vmat-1; solute carrier family 18 (vesicular monoamine), member 1. Slc18a1,25693, anti-VMAT1
Gene Symbol: Slc18a1
Entrez Gene: 25693 Rat