The ImmunoStar NK3R antiserum was quality control tested using standard immunohistochemical methods in rat hypothalamus using biotin/avidin-HRP techniques.
Specificity of the antiserum was demonstrated by soluble pre-adsorption and Western blot. Western blot analysis of crude rat brain homogenate demonstrates two immunoreactive bands of approximately 80 and 115 kD.
Tissue staining is completely eliminated by pretreatment of the diluted antibody with 25 mg of rat NK3R peptide residues 438-452.
Photo Description: IHC image of neurons staining for the neurokinin 3 receptor in the rat hypothalamus. The tissue was fixed with 4% formaldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
Quantity / Volume: 100 µL
Reacts With: Rat
Alternate Names: Tachykinin receptor 3; TACR3; NK-3 receptor; NKR, Neurokinin B receptor; Neuromedin-K receptor; TAC3R; Tachikin 3; Nmkr, anti-NK-3
Gene Symbol / ID, Accession #: Tacr3, 24808
Availability: In Stock