Insulin Antibody

$210.00

Product ID: 20056

*All Products sold by immunoStar are Tax Exempt.

Description

The ImmunoStar Insulin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat pancreatic beta cells using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/3000-1/5000 in PBS/0.3% Triton X-100 – biotin/avidin-HRP Technique.

Immuostaining is completely abolished by soluble pre-adsorption with bovine insulin at 10 µg per mL of diluted antiserum.

Photo Description: IHC image of beta cells in the islets of Langerhans of the rat pancreas staining for insulin (above) and counterstained with hemotoxylin (below). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for frozen sectioning. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Reacts With: Bubalus Bubalis (Buffalo), Cattle, Chick, Cow, Heifer (Cow), Horse, Human, Lizard, Macaque, Manducta Sexta (Moth), Monkey, Mouse, Pig, Rat, Sheep

Availability: In Stock

Alternate Names:  anti-Insulin

Gene Symbol:  INS

RRID:  AB_572246

Database Links:
Entrez Gene: 102396436   Bubalus Bubalis (Buffalo)
Entrez Gene: 280829         Cattle
Entrez Gene: 396145         Chick
Entrez Gene: 103567614    Horse
Entrez Gene: 3630             Human
Entrez Gene: 102133184    Macaque
Entrez Gene: 103239029    Monkey
Entrez Gene:16333            Mouse
Entrez Gene: 397415         Pig
Entrez Gene: 101719971    Rat
Entrez Gene: 105613195    Sheep

Technical Sheets

This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.

Download Data Sheet

Download MSDS

Reviews

Want to leave a review? Please click here to send us your review.

No Reviews Found.