Description
The ImmunoStar Insulin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat pancreatic beta cells using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/3000-1/5000 in PBS/0.3% Triton X-100 – biotin/avidin-HRP Technique.
Immuostaining is completely abolished by soluble pre-adsorption with bovine insulin at 10 µg per mL of diluted antiserum.
Photo Description: IHC image of beta cells in the islets of Langerhans of the rat pancreas staining for insulin (above) and counterstained with hemotoxylin (below). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for frozen sectioning. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen
Host: Rabbit
Quantity / Volume: 100 µL
State: Lyophilized Whole Serum
Reacts With: Bubalus Bubalis (Buffalo), Cattle, Chick, Cow, Heifer (Cow), Horse, Human, Lizard, Macaque, Manducta Sexta (Moth), Monkey, Mouse, Pig, Rat, Sheep
Availability: In Stock
Alternate Names: anti-Insulin
Gene Symbol: INS
RRID: AB_572246
Database Links:
Entrez Gene: 102396436 Bubalus Bubalis (Buffalo)
Entrez Gene: 280829 Cattle
Entrez Gene: 396145 Chick
Entrez Gene: 103567614 Horse
Entrez Gene: 3630 Human
Entrez Gene: 102133184 Macaque
Entrez Gene: 103239029 Monkey
Entrez Gene:16333 Mouse
Entrez Gene: 397415 Pig
Entrez Gene: 101719971 Rat
Entrez Gene: 105613195 Sheep
