The antibody produces strong labeling of GluR1 at dilutions of 1/4,000 – 1/6,000 using biotin-streptavidin peroxidase technique in rat cortex and hippocampus. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.
Western blot analysis of GluR1 transfected cells and rat brain homogenates the antibody specifically labels a single band at approximately 102 kD. Western blot analysis of GluR2, 3, 4, 4C, 5, 6, and 7 transfected cells revealed no immunolabeling. Immunolabeling of the above non-NMDA transfected cells demonstrates specificity for GluR1. Additionally, immunolabeling for GluR1 is completely abolished by pre-adsorption with synthetic rat GluR1 (894-907) at 5 µg per mL of diluted antibody.
Photo Description: IHC image of neurons staining for GluR1 in the rat cortex (above) and in a low magnification image of the rat hippocampus (below). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
Quantity / Volume: 100 µL
Reacts With: Rat
Alternate Names: AMPA-selective glutamate receptor 1; AMPA1; GLUH1; GluR-A; GluR-K1; Glutamate receptor ionotropic,AMPA1; Glutamate receptor ionotropic, kainate 1; GluK1; GluA1; glutamate receptor, ionotropic, AMPA1 (alpha 1), anti-GluR1
Gene Symbol: Gria1
Entrez Gene: 50592 Rat