The antibody produces strong labeling at dilutions of 1/120 – 1/150 (which is equivalent to 1/12,000 – 1/15,000) using biotin-streptavidin/HRP technique in rat cerebellum and thalamus. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.
Immunolabeling is completely abolished by preadsorption with excess GABA/BSA conjugate.
Photo Description: IHC image of neurons staining for GABA in the rat thalamus (above) and the rat cerebellum (below left, low magnification; below right, high magnification). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:20000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen and hemotoxylin counterstain in the rat thalamus.
Quantity / Volume: 100 µL
State: Lyophilized Whole Serum
Reacts With: Alligator, Armadillo, Bat, Bathynomus Doederleini (Crustacean/Isopod), Bird, Caenorhabditis Elegans Nematode (Roundworm), Cat, Drosophila (Fly), Drosophila Melanogaster (Fly), Ferret, Fish, Frog, Frog (Xenopus Laevis), Frog (Xenopus Tectum), Gerbil, Guinea Pig, Hamster, Human, Kitten (Cat), Monkey, Moth, Mouse, Parakeet, Pigeon, Pleurobranchaea Japonica (Sea Slug), Rabbit, Racoon, Rat, Slug, Snail, Turtle, Zebra Finch (Bird)
Availability: In Stock
Alternate Names: anti-GABA