CGRP (Calcitonin Gene Related Peptide) Antibody

$275.00

Product ID: 24112

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Description

The antibody has a proven and strong Biotin-Streptadvidin/HRP staining at a 1/2000-1/4000 dilution in rat amygdala, and spinal cord.

The specificity of the antiserum was evaluated by soluble pre-adsorption with the peptides in question at a final concentration of 10-5M. CGRP immunolabeling was completely abolished by pre-adsorption with rat α -CGRP and partially eliminated by pre-adsorption with rat Ÿ -CGRP. Pre-adsorption with the following peptides resulted in no loss of immunostaining: rat amylin, rat adrenomedulin, calcitonin, neurotensin, somatostatin, substance P, leucine enkephalin, methionine enkephalin, VIP, CCK-8, vasopressin and neuropeptide Y.

Photo Description: IHC image of the dorsal horn of the rat spinal cord staining for CGRP (above) and low magnification image of a coronal spinal cord section staining for CGRP (below). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for frozen sectioning. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:4000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Reacts With: Bullfrog, Cat, Dog, Hamster, Human, Mouse, Mudpuppy (Necturus Maculosus), Ovine (Sheep), Pig, Platypus, Ram (Sheep), Rat

Availability: In Stock

Alternate Names:  anti-CGRP

RRID:  AB_572217

Immunogen: rat alpha-CGRP

Gene Symbol: Calca

NCBI Gene Aliases: CAL6,CGRP,Cal1,Calc,RATCAL6,calcitoni

Database Links:
Entrez Gene:  101095582    Cat
Entrez Gene:  403946          Dog
Entrez Gene:  100772276    Hamster
Entrez Gene:  796               Human
Entrez Gene:  12310           Mouse
Entrez Gene:  100294596    Ovine (Sheep)
Entrez Gene:  100579174    Pig
Entrez Gene:  100579174    Platypus
Entrez Gene:  24241           Rat

Technical Sheets

This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.

Download Data Sheet

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Reviews

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Great antibody

Great signal-to-noise ratio. Works in wholemount and cryosections in spinal cord, skin, and gut.


Works great

This antibody works great on my tissue. With 1:500 to 1:1000 dilution, the result is superb!