The antibody is provided as 100 uL of affinity purified serum in PBS (0.02 M sodium phosphate with 0.15 M sodium chloride, pH 7.5) with 1% BSA (bovine serum albumin), and 0.02% sodium azide.
The ImmunoStar 5HT 6-receptor antibody was quality control tested using standard immunohistochemical methods. The antiserum demonstrates significant labeling of rat cortex, amygdala and hippocampus and other areas using indirect immunofluorescent and biotin/avidin-HRP techniques. The addition of intensifying reagents such as nickel ammonium sulfate to the chromogen solution will approximately double the dilution factor as recommended. Immunolabeling is completely abolished by preadsorption with synthetic rat 5HT6 receptor (CLERPPGTPRHPPGPPLW).
Immunolabeling of western blot revealed a single band of approximately 53kD. Due to the difficulty with receptor antibodies, western blot applications are not warranted and are included as specificity information only.
Quantity / Volume: 100 uL State: Liquid
Reacts With: Rat