The antibody has a proven strong Biotin-Streptavidin/HRP staining at a 1/8000-1/10,000 dilution in rat amygdala, cortex, and suprachiasmatic nucleus.
The specificity of the antiserum was examined by soluble pre-adsorption with the peptides in question at a final concentration of 10-5 M. VIP immunolabeling was completely abolished by pre-adsorption with VIP. Pre-adsorption with the following peptides resulted in no reduction of immunostaining: Secretin, gastric inhibitory polypeptide, somatostatin, glucagon, insulin, ACTH, gastrin 34, FMRF-amide, rat GHRF, human GHRF, peptide histidine isoleucine 27, rat pancreatic polypeptide, motilin, peptide YY, substance P, neuropeptide Y, and CGRP.
The histochemical antibody for VIP is generated in a rabbit against porcine VIP conjugated to bovine thyroglobulin with carbodiimide. The antibody is provided as 100 µL of lyophilized whole serum, and 0.09% sodium azide.
Photo Description: IHC image of rat cortex (above) and low magnification image of rat striatum and amygdala staining for VIP (below). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
Quantity / Volume: 100 µL
State: Lyophilized Whole Serum
Reacts With: Avian (Bird), Bat, Bubalus Bubalis (Buffalo), Bullfrog, Canine, Cat, Chick, Chicken, Dog, Dove, Fish, Fowl, Fox, Frog, Gilt (Pig), Goldfish, Guinea Pig, Hamster, Hedgehog, Hen, Human, Lizard, Mink, Mole, Mollusk, Monkey, Mouse, Mudpuppy (Necturus Maculosus), Ovine, Pig, Pigeon, Platypus, Possum, Rabbit, Raccoon, Ram, Rat, Scorpaena Porcus (Fish), Sea Lamprey, Sheep, Shrew, Snake, Sparrow, Sparus Aurata (Fish), Squirrel, Starfish, Sting Ray, Suncus Murinus, Tadpole, Turkey, Turtle, Worm (Spionidae), Zebra Finch, Zebrafish
Availability: In Stock
Preabsorption Control: Available at Sigma Aldrich, Catalog # V6130
Alternate Names: Vasoactive intestinal polypeptide; VIP peptides, anti-VIP
Gene Symbol: VIP
Entrez Gene: 102254866 Bat
Entrez Gene: 100217965 Bird
Entrez Gene: 102407426 Buffalo
Entrez Gene: 101101640 Cat
Entrez Gene: 396323 Chicken
Entrez Gene: 484038 Dog
Entrez Gene: 102095706 Dove
Entrez Gene: 102898768 Fox
Entrez Gene: 373781 Frog
Entrez Gene: 100735454 Guinea Pig
Entrez Gene: 100773469 Hamster
Entrez Gene: 7432 Human
Entrez Gene: 101713745 Mole
Entrez Gene: 703566 Monkey
Entrez Gene: 22353 Mouse
Entrez Gene: 100500718 Pig
Entrez Gene: 100352512 Rabbit
Entrez Gene: 100145884 Ram
Entrez Gene: 117064 Rat
Entrez Gene: 102066375 Sparrow
Entrez Gene: 101977899 Squirrel
Entrez Gene: 102457597 Turtle
Entrez Gene: 795653 Zebrafish
This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records. Download Data Sheet
Want to leave a review? Please click here to send us your review.
Wonderful VIP Antibody
Mice perfused with 10% Formalin, post-fixed for 24 hrs.
Vibratome sections (50µm and 100µm) cut and stored in Anti-freeze @ -20°C until usage. 20 minute Antigen retrieval in 85°C Na-Citrate. Primary incubation in 4% Donkey serum in PBST for 4 days, secondary incubation overnight (tried several fluorophore-coupled secondaries (e.g. Alexa 405 and Alexa 488 coupled). We tried several primary dilutions from 1/500 to 1/5000 and cells were clearly labelled in all cases.
As expected – staining not only in soma but throughout the cells, which can be mistaken as somewhat high background but these cortical VIP cells just are quite arborized. Very happy with the performance of this antibody!
Rbt anti-VIP review
We used VIP (product no. 20077) to stain for VIP positive neurons in the Pancreas and celiac mesenteric ganglion (CMG). For consistent and positive staining, we used a concentration of 1:500.
Animals were perfused with 4% PFA and tissue was post-fixed in 4%PFA overnight and then put into sucrose the following day. Tissue was sectioned at 20um on a Cryostat and mounted onto subbed slides.
Tissue was blocked with a 4% Goat Serum/Superblocker for 1hr. Primary antibody was added to the slides for 16hrs at room temperature. Slides were then washed 4 times for 15 min with a 1% triton X-100/Goat Serum/PBS solution. Secondary antibodies were then added for 3hrs at room temperature. Washed again 4 times for 15 min and then coverslipped.
Antibody labeled axon processes in CMG and cells in Pancreas.
Ty Redler, BS; OPS Sponsored Projects, Non-Clerical
Dr. Richard Johnson’s Immunohistochemistry Lab
University of Florida
College of Veterinary Medicine
Very good labeling!
We used the VIP antibody (Product ID: 20077) to stain for VIP positive neurons in mouse brain.
As recommended by Immunostar, after transcardial perfusion, brains were postfixed for 1.5 hours at 4°C in 4% paraformaldehyde. The following day, brains were sliced in the coronal plane on a vibratome at 40 μm and sections were collected in PBS. The tissue was incubated with primary antibody between 18–24 hours at 4°C, using 0.3% triton X-100, and 1% blocking serum.
We found that the most consistent staining for immunofluorescence was achieved with a 1:1000 dilution of the primary antibody. We evaluated antibody staining in the cortex and suprachiasmatic nuclei. The antibody strongly labeled cell bodies and also stained neuronal processes. Staining patterns matched previously published results.
Marina Silveira, Ph.D. and Michael Roberts, Ph.D.
Kresge Hearing Research Institute
University of Michigan
We have used this antibody to stain for VIP positive neurons (also stains processes) in frozen tissue sections and whole mount preparations of mouse intestine. We get consistent staining at 1:750.
4% PFA/PBS for 1.5 hrs, cryoprotected in 30% sucrose until they sink, OCT embedded, and sectioned.
For whole mounts, we open up intestine and fix in 4% PFA/PBS for 1.5 hrs.
Rehydrate in PBS, block with HINGS, stain in primary @4C O/N.
Wash in PBST, stain in primary with HINGS and DMSO @4C 48 hrs.
Wash with PBS, stain with Alexafluor secondaries (Invitrogen), wash with PBS, and DAPI mount.
Wash in PBST, stain in secondary with HINGS and DMSO @RT 48 hrs.
Optional: proceed with MeOH dehydration and optical clearing.
Meenakshi Rao, MD PhD
Assistant Professor of Pediatrics
Columbia University Medical Center
New York, NY 10032
Worked like a charm
After failed several experiments by using other antibodies, we applied VIP antibody (20077) for mouse brain tissue sections for suprachiasmatic nucleus immunohistochemistry.
It turned out that this antibody worked like a charm on my frozen sections. This is definitely an excellent VIP antibody.
Dr. David Ma, Research scientist