The ImmunoStar GAT-2 antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat retina and leptomeninges using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/500 – 1/1,000 in PBS/0.3% Triton X-100 – biotin/avidin-HRP Technique. The antiserum has been characterized as specific to GAT-2; please see references listed below. GAT-2 immunolabeling is completely abolished by soluble pre-adsorption with synthetic rat GAT-2 (594-602) at a concentration of 10-5 M.
Photo Description: IHC image of rat retina staining for GABA transporter 2 (GAT-2). The tissue was fixed with 4% formaldehyde/0.05% glutaraldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:1000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
Quantity / Volume: 100 µL
State: Lyophilized Whole Serum
Reacts With: Rat
Availability: In Stock
Alternate Names: Sodium- and chloride-dependent GABA transporter 2; Solute carrier family 6 member 13; Gabt2; solute carrier family 6 (neurotransmitter transporter, GABA), member 13, anti-GAT2
Gene Symbol: Slc6a13
Entrez Gene: 171163 Rat