Gastrin Releasing Peptide (GRP) / Bombesin Antibody

$300.00

Product ID: 20073

*All Products sold by immunoStar are Tax Exempt.

Description

The ImmunoStar Gastrin Releasing Peptide antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat dorsal horn of spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/1,000-1/2,000 in PBS/0.3% Triton X-100 – Bn/Av-HRP Technique. Staining is completely eliminated by pretreatment of 1 mL of diluted antibody with 50 µg of bombesin.

Photo Description: IHC of at stomach staining for gastrin releasing peptide. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for frozen sectioning. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:2000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen and counterstained with hematoxylin

Host: Rabbit

Quantity / Volume: 100 µL

State: Lyophilized Whole Serum

Reacts With:  Chick, Hamster, Human, Mouse, Newt, Pigeon, Quail, Rabbit, Ram (Sheep), Rat, Zacco Platypus (Minnow)

Availability: In Stock

Preabsorption Control: Available at irbiologicals, Catalog # IRCP069.  Sequence: Glp-Gln-Arg-Leu-Gly-Asn-Gln-Trp-Ala-Val-Gly-His-Leu-Met-NH

Alternate Names:  BN; GRP-10; proGRP; preproGRP; gastrin-releasing peptide, anti-GRP, Bombesin, anti-Bombesin

Gene Symbo:  GRP

RRID: AB_572221

Database Links:
Entrez Gene: 425213        Chick
Entrez Gene: 100769410   Hamster
Entrez Gene: 2922            Human
Entrez Gene: 225642         Mouse
Entrez Gene: 102093899    Pigeon
Entrez Gene: 107325907    Quail
Entrez Gene: 103349406    Rabbit
Entrez Gene: 443332         Ram (Sheep)
Entrez Gene: 171101         Rat

Technical Sheets

This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.

Download Data Sheet

Download MSDS

Reviews

Want to leave a review? Please click here to send us your review.

Scientist

This is our first time using this antibody (Prod ID: 20073) from ImmmunoStar. It works beautifully in our immunohistochemical assays on mouse dorsal tissues. We used 1:2000 dilutions. It is one of the best ones among similar antibodies we have tested from other vendors. We would like to recommend this item to our colleagues.

Jason Zhang, Scientist
University of California-San Diego