The ImmunoStar gamma amino butyric acid antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat thalamus and cerebellum using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/15,000- 1/20,000 in PBS /0.3% Triton X-100 – biotin/avidin-HRP technique.
The specificity of the antiserum for GABA was evaluated using a competitive inhibition ELISA. While conjugates of GABA completely eliminate labeling, a 1000 fold excess of the following conjugates could not inhibit the antiserums ability to bind GABA conjugate: glutamate, aspartate, beta alanine, tyrosine, taurine, glycine and alanine.
Photo Description: IHC image of neurons staining for GABA in the rat thalamus (above) and the rat cerebellum (below left, low magnification; below right, high magnification). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:20000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen and hemotoxylin counterstain in the rat thalamus.
Quantity / Volume: 100 µL
State: Lyophilized Whole Serum
Reacts With: Alligator, Armadillo, Bat, Bathynomus Doederleini (Crustacean/Isopod), Bird, Caenorhabditis Elegans Nematode (Roundworm), Cat, Drosophila (Fly), Drosophila Melanogaster (Fly), Ferret, Fish, Frog, Frog (Xenopus Laevis), Frog (Xenopus Tectum), Gerbil, Guinea Pig, Hamster, Human, Kitten (Cat), Monkey, Moth, Mouse, Parakeet, Pigeon, Pleurobranchaea Japonica (Sea Slug), Rabbit, Racoon, Rat, Slug, Snail, Turtle, Zebra Finch (Bird)
Availability: In Stock
Alternate Names: anti-GABA
Gene Symbol: Rdl
Entrez Gene ID: 39054
NCBI Gene Aliases: CG10537,DmRDL,DmRdl,DmelCG10537,GABA,GABA[[A]],”GABA[[A]]receptor” GABAr, LCCH1, RDL, Rd1, gaba,rd