The antibody has a proven maximum biotin-avidin/HRP staining at a 1/1000 – 1/4000 dilution in rat cortex, hippocampus and hypothalamus.
The antiserum has been characterized as specific to calretinin; please see references listed below. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended
Photo Description: IHC image of neurons staining for calretinin in the rat cortex (above) and the rat dentate gyrus (below). The tissue was fixed with 4% formaldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:4000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
Quantity / Volume: 100 µL
State: Lyophilized Whole Serum
Reacts With: Rat
Availability: In Stock
Immunogen: Chick Calretinin fusion protein
Gene Symbol: CALB2
Entrez Gene: 117059 Rat