The ImmunoStar 5-HT5A Receptor was quality control tested using standard immunohisto-chemical methods. The antiserum demonstrates strongly positive labeling of rat cortex and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/200 – 1/400 in PBS/0.3% Triton X-100 – Bn/Av-HRP. Intensification methods such as nickel will approximately double the dilution factor as recommended.
The antibody was characterized by immunoblotting and immunohistochemistry. Immunoblots of rat brain extracts revealed the presence of two bands at molecular weights of 41 and 47 kD. The lower weight band agrees with the calculated molecular weight based on amino acid sequence. The higher weight may represent glycosylated receptor protein. Due to the difficulty with receptor antibodies, western blot applications are not warranted and are included as specificity information only.
Immunohistochemical staining of rat brain correlates well with Northern blot analysis and in situ hybridization studies. Immunolabeling is completely abolished by preadsorption with synthetic rat 5-HT5A receptor (17-34). BlastP database sequence homology searches indicate that the amino acid sequence is unique to rat and mouse 5-HT5A receptor.
Photo Description: IHC image of neurons staining for the 5-HT5A receptor in the rat hippocampus (above) and the rat cortex (below). The tissue was fixed with 4% formaldehyde/0.05% glutaraldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:500 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
Quantity / Volume: 100 µL
Reacts With: Clam, Rat
Availability: In Stock
Alternate Names: 5-hydroxytryptamine receptor 5A; 5-HT5A; REC17; 5ht5a,MR22; 5-hydroxytryptamine (serotonin) receptor 5A, G protein-coupled, anti-5-HT5a
Immunogen: Rat 5-HT5A receptor (17-34)
Gene Symbol: Htr5a
Entrez Gene: 25689 Rat