LHRH (Luteinizing Hormone Releasing Hormone) / GnRH Antibody

$250.00

Product ID: 20075

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Description

The antibody produces a strong postive labeling of LHRH / GnRH at dilution of 1/2,000 – 1,4,000 using biotin-streptavidin/HRP in rat hypothalamus (median eminence). Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.

The above image is of LHRH (GnRH) terminals in rat median eminence, ryostat sections, ABC / DAB detection.

Staining is completely eliminated by pretreatment of the diluted antibody with 5 µg of LHRH per mL of diluted antiserum.

Photo Description: IHC image of staining for LNRH/GnRH in the rat median eminence. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:4000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Reacts With: Bat, Carp, Catfish, Chick, Chicken, Clarias Batrachus (Catfish), Fish, Frog, Frog (Green Treefrog), Gerbil, Guinea Pig, Guinea Pig, Hagfish, Hamster, Human, Lizard, Mole Rat, Monkey, Mouse, Mudpuppy (Necturus Maculosus), Octopus, Pig, Rat, Salamander, Snail, Snail (Bulla Gouldiana), Squid, Trout

Availability: In Stock

Alternate Names:  Gonadotropin-Releasing Hormone; Gonadorelin; Luliberin I; Gonadoliberin I; progonadoliberin-1; GRH, anti-LHRH

Gene Symbol:  GNRH1

RRID:  AB_572248

Database Links:
Entrez Gene:103295055    Bat
Entrez Gene: 770134        Chick
Entrez Gene: 100135026   Frog
Entrez Gene: 100135531   Guinea Pig
Entrez Gene: 100774729    Hamster
Entrez Gene: 2796             Human
Entrez Gene: 101705387    Mole Rat
Entrez Gene: 613033         Monkey
Entrez Gene: 14714           Mouse
Entrez Gene: 397516         Pig
Entrez Gene: 25194           Rat
Entrez Gene: 100135917    Trout

Technical Sheets

This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.

Download Data Sheet

Download MSDS

Reviews

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Great antibody – clear and consistent staining

Used on paraffin embedded brain tissue, sectioned at 10 microns, and placed on charged slides.

Protocol used:

Day 1
Slide rehydration
Avidin/biotin blocking
2 x 3 minutes PBS-T
Blocking with 5% goat serum in PBS-T
Incubated overnight with the antibody at a 1:1000 concentration

Day 2
30 minutes in secondary antibody (1:300)
2 x 3 minutes PBS-T
30 minutes in ABC
2 x 3 minutes PBS-T
5 minutes in Di water
Staining developed with DAB

Used in: Hoffmann HM, Larder R, Lee JS, Hu RJ, Trang C, Devries BM, Clark DD, Mellon PL. Differential CRE Expression in Lhrh-cre and GnRH-cre Alleles and the Impact on Fertility in Otx2-Flox Mice. Neuroendocrinology. 2019;108(4):328-342. doi: 10.1159/000497791. Epub 2019 Feb 10. PMID: 30739114; PMCID: PMC6753941.

Submitted by:

Brooke Devries
Michigan State University
426 Auditorium Road
East Lansing, MI 48824


Highly recommend — very specific staining with no background

I use the LHRH Antibody for immunohistochemistry on mouse embryonic tissue. I use cryostat sections of PFA fixed mouse heads, included in OCT.

I cut 10-20 microns sections, dry them and the process them as following: Protocol: 1hr blocking (PBS/0.1% triton plus 10% NGS), incubate overnight with LHRH antibody diluted in PBS/0.1% triton, 1:400. Then washes and secondary antibody fluorescent. When used with secondary biotinylated antibody, I use LHRH antibody less diluted (1:4000).

My student is also using the LHRH antibody on paraffin sections, with the same results (and also in conjunction with Tyramide amplification kit).

Staining is very specific. Stronger on adult tissue because more GnRH expressed by the neurons producing it. There is no background. Fixation sensitive; long fixations are not recommended. I highly recommend this antibody.

Anna Cariboni, PhD
Department of Cell and Developmental Biology
University College London