The antibody has a proven strong Biotin-Streptavidin/HRP immunostaining at a 1/500-1/1000 dilution in rat hypothalamus and spinal cord.
The specificity of the antiserum was examined by soluble pre-adsorption with the peptides in question at a final concentration of 10-6M CCK-8 immunolabeling was completely abolished by pre-adsorption with CCK-8, gastrin 17 and gastrin 34. Pre-adsorption with the following peptides resulted in no reduction of immunostaining: α-CGRP, Ÿ-CGRP, neurotensin, somatostatin, substance P, leucine enkephalin, methionine enkephalin, VIP, neuropeptide Y, gastric inhibitory polypeptide, bombesin, glucagon, peptide YY, and FMRF amide.
Product Description: Low magnification IHC image of neurons staining for CCK-8 in the dorsal horn of the rat spinal cord (above), and in the rat hippocampus (below). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning (hippocampus) and frozen sectioning (spinal cord). Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:1000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
Quantity / Volume: 100 µL
State: Lyophilized Whole Serum
Reacts With: Blowfly, Bubalus Bubalis (Water Buffalo), Cat, Chick, Decapod Crustacea, Gerbil, Guinea Pig, Hamster, Human, Lizard, Lobster, Mollusk, Mollusk, Monkey, Mouse, Mudpuppy (Necturus Maculosus), Pigeon, Rabbit, Raccoon, Rat, Sheep, Snail, Snail (Bulla Gouldiana) , Sting Ray, Tree Frog, Trout, Zacco Platypus (Minnow)
Availability: In Stock
Preabsorption Control: Available at Sigma Aldrich, Catalog # C2901
Alternate Names: Cholecystokinin-8; CCK, anti-CCK
Immunogen: Sulfated CCK-8 (26-33)
Gene Symbol: CCK
Entrez Gene: 102415979 Bubalus Bubalis (Water Buffalo)
Entrez Gene: 101096837 Cat
Entrez Gene: 414884 Chick
Entrez Gene: 100735029 Guinea Pig
Entrez Gene: 100772438 Hamster
Entrez Gene: 885 Human
Entrez Gene: 105722552 Monkey
Entrez Gene: 12424 Mouse
Entrez Gene: 102094098 Pigeon
Entrez Gene: 100355199 Rabbit
Entrez Gene: 25298 Rat
Entrez Gene: 101116683 Sheep
Entrez Gene: 100136632 Trout