Vasopressin Antibody

Vasopressin Antibody
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Price: $275.00
Product ID : 20069

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Description

The antibody has a proven strong biotin-streptavidin/HRP staining at a 1/2000 - 1/4000 dilution in rat hypothalamus.

Staining is completely eliminated by pretreatment of the diluted antibody with 10 µg/mL of arginine vasopressin. Pre-adsorption with as much as 100 µg/mL of oxytocin had no effect on immunolabeling.

Photo Description: IHC image of rat hypothalamus staining for vasopressin. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. 

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Reacts With: Anole, Bluehead Wrasses (Fish), Chicken, Clarias Batrachus (Catfish), Cockroach, Ewe / Sheep, Hamster, Human, Hydra Vulgaris (Polyp), Locust, Mole Rat, Monkey, Mouse, Pigeon, Rat, Shrew, Squirrel

Availability: In Stock

Alternate Names:  Vasopressin (Arg); Antidiuretic Hormone; Arginine vasopressin; Vasopressin-neurophysin 2-copeptin; ARG-vasopressin; prepro-AVP-NPII; neurohypophyseal; vasopressin-neurophysin II-copeptin; prepro-arginine-vasopressin-neurophysin II; AVP-NPII; VP; ADH; ARVP; AVRP, anti-vasopressin

Gene Symbol:  AVP

RRID:  AB_572219

Database Links:
  Entrez Gene: 396101         Chicken
  Entrez Gene: 443477         Ewe / Sheep
  Entrez Gene: 551               Human
  Entrez Gene: 101717720    Mole Rat 
  Entrez Gene: 717101         Monkey
  Entrez Gene: 11998           Mouse
  Entrez Gene: 24221           Rat
  Entrez Gene: 102870182    Shrew

Reviews

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Reviewed by forbeslorman
09/09/2019 - 09:25:51 AM
Great antibody

This antibody is the best! I use it for visualizing vasopressin within the rat brain. 1:10,000 dilution results in beautiful staining of the magnocellular regions of the PVN and SON, as well as some staining in the BST and amygdala. My protocol is:

Day 1

_ _ _ Wash 3x5 min TBS

____ 0.1% sodium borohydride in TBS 15 min

_ _ _ Wash 3x5 min TBS

____ 20% normal serum and 3% H202 in TBS 1 hr

____ Primary antibody 1:10K with 2% normal serum in TBS -Triton over 3 nights @ 4º


Send good thoughts over the next 3 days


Day 2

_ _ _ Wash 3x5 min TBS- Triton

____ Secondary antibody 1:500 in TBS-Triton w/ 0.5% gelatin 90 min

____ Make ABC during last 20 min of 2o (25 ul each of A & B per 10 ml TBS)

_ _ _ Wash 1x5 min TBS- Triton, 2x5 min TBS

____ ABC 60 min

_ _ _ Wash 3x5 min TBS-Triton

____ Visualize with Vector SG (6 drops of each reagent per 10 ml TBS-T) + 0.5% gelatin

_ _ _ Wash 3x5 min TBS


Submitted by:

Robin Forbes-Lorman
Biology Department
Ripon College
Ripon, WI
Reviewed by banerjeelab
12/09/2015 - 10:25:52 AM
Excellent for IHC

This is a high-quality antibody that we have found to consistently produce beautiful, specific staining. We used a 1:1,000 concentration in cryosectioned mouse brain slices following blocking with 10% serum. Labeled cell bodies and fibers were clearly discernable at 20x magnification in the hypothalamus. Highly recommended.
Reviewed by user
12/18/2012 - 01:11:07 PM
Beautiful cells

We have used your Vasopressin antibody (Product ID: 20069) in many of our studies and have found in to be a very specific and clear antibody. The staining is great and the cells are beautiful.

We use a total 1:5000 concentration. It is mixed with 10% serum, 10% biotin diluted in 80% PBS-T and incubated for 48-72 hours at 4 degrees C. We used brain tissue from Thamnophis sirtalis (Red-sided garter snake).

Submitted by:

Ashley Maine
Lutterschmidt Lab
Portland State University
Department of Biology
Portland, OR 97207

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MSDS

This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.

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