nNOS:C-Terminal (Neuronal Nitric Oxide Synthase)

nNOS:C-Terminal (Neuronal Nitric Oxide Synthase)
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Price: $350.00
Product ID : 24287

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The ImmunoStar neuronal nitric oxide synthase C-terminal antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus, striatum, cortex and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/8,000 - 1/12,000 in PBS/0.3% Triton X-100 - Bn/Av-HRP Technique.

By Western blot analysis of brain homogenates the antibody specifically labels a band of approximately 155 kD. Immuno-labeling is completely abolished by pre-adsorption with synthetic human nNOS (1419-1433) at 5 µg per mL of diluted antibody. No cross reactivity with other forms of NOS were observed. 

Photo Description: IHC image of a single neuron staining for the C-terminal of nNOS in the rat cortex (above), along with low magnification of cortex (below left), neurons in the rat dentate gyrus (below middle) and in the striatum (below right). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:10000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen, below middle image with nickel stain.

 nNos C-Terminal cortex #24287    nNOS-C-terminal striatum #24287

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Reacts With: Cat, Chick, Ferret, Guinea Pig, Hamster, Human, Monkey, Mouse, Opossum, Rat, Shrew, Squirrel Monkey, Vole, Zebrafish

Availability: In Stock

Alternate Names:  Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS; Nitric Oxide Synthase; bNOS; Nitric oxide synthase-brain; N-NOS; Peptidyl-cysteine S-nitrosylase; IHPS1; NC-NOS; nitric oxide synthase 1 (neuronal), anti-nNos:C-Terminal

Gene Symbol:  NOS1

RRID:  AB_572256

Database Links:  

  Entrez Gene: 101100342    Cat
  Entrez Gene: 427721         Chick
  Entrez Gene: 101692439    Ferret
  Entrez Gene: 100729682    Guinea Pig
  Entrez Gene: 100758127    Hamster
  Entrez Gene: 4842             Human
  Entrez Gene: 693781          Monkey
  Entrez Gene: 18125            Mouse
  Entrez Gene: 100013712     Opossum
  Entrez Gene: 24598            Rat
  Entrez Gene: 102850179     Shrew
  Entrez Gene: 101034544     Squirrel Monkey 
  Entrez Gene: 101984335     Vole
  Entrez Gene: 60658            Zebrafish


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Reviewed by bnovitch
01/28/2019 - 12:40:37 PM
A go to antibody for PGC spinal motor neurons

My lab has used this antibody for many years to distinguish preganglionic motor neurons in the embryonic mouse spinal cord. It is very clean and concentrated. We generally use at a 1:10,000 dilution. An example can be found in one of our papers doi: 10.1016/j.neuron.2008.06.025 (see Fig 1U).

We process our spinal cord tissues by immersion in cold 4% PFA/PBS for 1.5-2 hours depending on the size/age of the sample, wash 3X with cold PBS, and cryoprotect in 30% sucrose before cryosectioning.

Antibody staining is achieved by thawing slides, overlaying with blocking solution (PBS, 0.1% TX100 , 1% Horse (or Donkey) serum) for 15 minutes. Then add primary antibodies diluted in the blocking solution. Incubate overnight at 4 degrees. Next day wash 3X with PBST (PBS with 0.1% TX100), and add fluorescent secondary antibodies (we generally use those from Jackson Immunoresearch) for ~1-2 hours at room temp. Wash 3X with PBST and coverslip using Prolong Gold (Invitrogen).
Reviewed by ld2596
03/19/2015 - 02:10:46 PM
Fantastic antibody!

We have used this antibody to stain for nNOS positive neurons (also stains some processes) in frozen tissue sections and whole mount preparations of mouse intestine. We get consistent staining at 1:1500 although the staining of the neurons is so strong this can probably be dropped, even for quantification purposes!

Tissue prep:
4% PFA/PBS for 1.5 hrs, cryoprotected in 30% sucrose until they sink, OCT embedded, and sectioned.
For whole mounts, we open up intestine and fix in 4% PFA/PBS for 1.5 hrs.

IF protocol:
Rehydrate in PBS, block with HINGS, stain in primary @4C O/N.
Wash in PBST, stain in primary with HINGS and DMSO @4C 48 hrs.

Wash with PBS, stain with Alexafluor secondaries (Invitrogen), wash with PBS, and DAPI mount.
Wash in PBST, stain in secondary with HINGS and DMSO @RT 48 hrs.
Optional: proceed with MeOH dehydration and optical clearing.

Meenakshi Rao, MD PhD
Assistant Professor of Pediatrics
Columbia University Medical Center
New York, NY 10032

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This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.

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