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The ImmunoStar Neuropeptide Y Y1 Receptor was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat cortex, arcuate and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/500 - 1/1000 in PBS - Bn/Av-HRP detection.
The three black and white images are confocal image of spinal cord neurons labeled with antibody 24506 against the neuropeptide Y Y1 receptor using immunofluorescence. More details in Marvizon et al., 2019, Neuropharmacology 158: 107732. Courtesy of Juan Carlos Marvizon, Ph.D., UCLA.
The antibody was characterized by immunohistochemistry and Western blot. Western blot showed one immunoreactive band of 40 kD and a single high molecular weight band, presumably a precursor molecule.
Preincubation of the antibody with an excess of the synthetic peptide blocked staining. Immunohistochemical staining of rat brain correlates well with Northern analysis, in situ hybridization and receptor autoradiography. BlastP database sequence homology searches confirmed that this sequence is unique to rat, mouse and human NPY Y1 receptors.
Photo Description: IHC image of the rat cortex staining for the neuropeptide Y Y1 recptor. The tissue was fixed with 4% formaldehyde/0.05% glutaraldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
Quantity / Volume: 100 µL
Reacts With: Hamster, Human, Mouse, Rat
Availability: In Stock
Alternate Names: Neuropeptide Y receptor 1; NPY receptor 1; NPYR; NPYY1; NPY1-R; Neuropeptide Y receptor type 1; FC5; NPYR1, neuropeptide Y1 receptor, anti-NPYY1
Gene Symbol: neuropeptide Y receptor Y1, anti-NPYY1
09/30/2019 - 11:46:52 AM
Performed extremely well for immunofluorescence
It was used at a dilution of 1:500 in 10% normal serum, followed by several secondary antibodies coupled to Alexa Fluors 448, 546 or 568.
The antibody produced a crisp staining located at the cell surface, except that in tissue incubated with neuropeptide Y or after inducing the release of endogenous neuropeptide Y the immunoreactivity became internalized in numerous endosomes in the cytoplasm. The internalization was blocked by the selective Y1 receptor antagonist BIBO3304. This provides indirect evidence for the specificity of the antibody.
Juan Carlos Marvizon
UCLA, VA Medical Center
Los Angeles CA 90073
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This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.Download MSDS