Description
The antibody has a proven strong biotin-streptavidin/HRP staining at 1/1,000-1/1,200 dilution in rat globus pallidus and amygdala.
Staining is completely eliminated by pretreatment with 100 µg of methionine enkephalin per mL of diluted antiserum. Pretreatment with 100 µg of leucine enkephalin only partially blocks staining.
Photo Description: High magnification of the globus pallidus with nickel preparation (above) and low magnification IHC image of methionine encephalin staining in the globus pallidus and amygdala for the rat brain (below). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:2000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
Host: Rabbit
Quantity / Volume: 100 µL
State: Lyophilized Whole Serum
Reacts With: Astyanax Jordani (Fish), Bird, Blowfly, Bovine, Bullfrog, Cat, Chick, Chicken, Cockroach, Cod, Crab, Dog, Fish, Frog, Gerbil, Goldfish, Guinea Pig, Hamster, Human, Lamprey, Leech, Lymnaea Stagnalis (Snail), Manducta Sexta (Moth), Monkey, Mouse, Octopus, Parrot, Pig, Pigeon, Rabbit, Rana Esculenta (Frog), Rana Pipiens (Leopard Frog), Rana Temporaria (Frog), Rat, Ratfish, Salamander, Salmon, Sea Bass (Fish), Shrew, Squirrel Monkey, Starling, Tapeworm, Turtle, Zebra Finch
Availability: In Stock
Alternate Names: Enkephalin (Met); Proenkephalin-A; Met-enkephalin; Opioid growth factor; OGF, anti-Methionine Enkephalin, Met Enk
Gene Symbol: PENK
RRID: AB_572250
Database Links:
Entrez Gene: 281387 Bovine
Entrez Gene: 768277 Cat
Entrez Gene: 421131 Chick
Entrez Gene: 100687307 Dog
Entrez Gene: 548461 Frog
Entrez Gene: 100379621 Guinea Pig
Entrez Gene: 101844627 Hamster
Entrez Gene: 5179 Human
Entrez Gene: 696649 Monkey
Entrez Gene: 18619 Mouse
Entrez Gene: 100152093 Pig
Entrez Gene: 102088743 Pidgeon
Entrez Gene: 100342780 Rabbit
Entrez Gene: 29237 Rat
Entrez Gene: 100196814 Salmon
Entrez Gene: 101552431 Shrew
Entrez Gene: 106851221 Starling
Entrez Gene: 102933698 Turtle
Entrez Gene: 100190068 Zebra Finch
