The ImmunoStar alpha melanocyte stimulating hormone antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat pituitary using biotin/avidin-HRP techniques.
Recommended primary dilution is 1/4000-1/6000 in PBS/0.3% Triton X-100 – Bn/Av-HRP Technique. Staining is completely eliminated by pretreatment of the diluted antibody with 100 ug/mL of alpha-MSH.
Photo Description: IHC image of neurons staining for MSH in the rat hypothalamus (above) and rat pituitary with nickel preparation (below). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for sectioning. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
Quantity / Volume: 100 µL
State: Lyophilized Whole Serum
Reacts With: Pig, Hamster, Human, Monkey, Mouse, Rabbit, Rat, Trout
Availability: In Stock
Alternate Names: POMC; proopiomelanocortin, anti-Alpha-MSH
Gene Symbol: POMC
Entrez Gene: 101087223 Cat
Entrez Gene: 496777 Frog (Xenopus Laevis)
Entrez Gene: 100720803 Guinea Pig
Entrez Gene: 100756311 Hamster
Entrez Gene: 5443 Human
Entrez Gene: 694430 Monkey
Entrez Gene: 18976 Mouse
Entrez Gene: 100344462 Rabbit
Entrez Gene: 24664 Rat
Entrez Gene: 100136771 Trout