The ImmunoStar 5-HT7 receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat cortex and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/100 – 1/300 in PBS – biotin/avidin-HRP Technique. Note: use of Triton X-100 or other detergents is not recommended.
The antibody was characterized by immunohistochemistry. Immunohistochemical staining of rat brain correlates well with Northern blot analysis, in situ hybridization and receptor autoradiography studies.Due to the difficulty with receptor antibodies, blot applications are not warranted and are included as specificity information only.
Immunolabeling is completely abolished by preadsorption with synthetic rat 5-HT7 receptor (8-23). BlastP database sequence homology searches indicate that the amino acid sequence is unique to rat 5-HT7A, 5-HT7B and 5-HT7C. There is also significant sequence overlap with the mouse and human forms.
Photo Description: IHC image of neurons staining for the 5-HT7 receptor in the rat brain cortex. The tissue was fixed with 4% formaldehyde/0.05% glutaraldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:500 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
Quantity/Volume: 100 µL
Reacts With: Cat, Grasshopper, Human, Hutchinsoniella Macracantha (Horseshoe Shrimp), Mouse, Pig, Rabbit, Rat
Availability: In stock
Alternate Names: 5-hydroxytryptamine receptor 7; 5-HT-X,GPRFO; 5Ht7; 5-hydroxytryptamine (serotonin) receptor 7, anti-5-HT 7
Immunogen: Rat 5-HT7 receptor (8-23)
Gene Symbol: Htr7