5-HT (Serotonin) / BSA Conjugate Control

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Price: $125.00
Product ID : 20081

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Description

Pre-adsorption of Serotonin antisera, diluted according to the antibody specification sheet, with 20 µg/ml Serotonin/BSA conjugate following the instructions below provides complete blockage of Serotonin immunolabeling. The conjugate is provided as 50 µg of lyophilized Serotonin creatinine sulfate coupled to BSA with paraformaldehyde. Also, this antiserum contains 0.09% sodium azide. Please read the instructions carefully before beginning the procedure. 

 

Host: N/A 

Quantity / Volume: 50 µg

State: Lyophilized

Reviews

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Reviewed by adacks
01/21/2013 - 11:45:25 AM
Good for pre-adsorbing rabbit anti-5HT antibody (#20080)

I used the 5HT-BSA conjugate to run a pre-adsorption control for the rabbit anti-serotonin antibody (#20080). 40mg/ml resulted in only very weak labeling of 1:4,000 and 1:10,000 dilutions of the rabbit anti-serotonin antibody, while all labeling was eliminated for dilutions of 1:20,000 and lower. Dilutions of 1:4,000 to 1:80,000 all provided bright labeling in positive controls in which the BSA-5HT conjugate was omitted. The application was immunocytochemistry of sectioned brain tissue from the bumble bee Bombus impatiens (see Paulk et al 2009, J. Comp. Neurol.).

Day 1:
____Incubate 40mg/ml 5HT-BSA conjugate with 1:4,000, 1:10,000, 1:20,000, 1:40,000 and 1:80,000 dilutions of the rabbit anti-serotonin antibody overnight at room temperature with mild agitation. In parallel, equal volumes of these dilutions in which the 5HT-BSA conjugate is omitted are also incubated under the same conditions to provide positive controls.
Day 2:
_____All samples are spun down at 14,000RPM for 15 minutes at 4oC
_____Supernatant is collected for use in standard 5HT labeling protocol.

Andrew Dacks
Assistant Professor
West Virginia University

Publications

PROD # ANTIBODY CITATION SPECIES REACTIVITY APPL METHOD RESEARCH AREA # CITED BY PUBMED ID
20081 5-HT (Serotonin) / BSA Conjugate Control De Girolamo, P., et al. "Co?localization of Trk neurotrophin receptors and regulatory peptides in the endocrine cells of the teleostean stomach." The Anatomical Record 256.3 (1999): 219-226. Sparus Aurata (Fish), Scorpaena Porcus (Fish) IHC 26 10521780
20081 5-HT (Serotonin) / BSA Conjugate Control J Comp Neurol. 2008 Dec 20;511(6):804-31. doi: 10.1002/cne.21857. Development of the serotoninergic system in the central nervous system of a shark, the lesser spotted dogfish Scyliorhinus canicula. Carrera I1, Molist P, Anadón R, Rodríguez-Moldes I. Fish IF 18925650
20081 5-HT (Serotonin) / BSA Conjugate Control Delgado, Nadia, Deborah Vallejo, and Mark W. Miller. "Localization of serotonin in the nervous system of Biomphalaria glabrata, an intermediate host for schistosomiasis." Journal of Comparative Neurology 520.14 (2012): 3236-3255. Snail 0 22434538
20081 5-HT (Serotonin) / BSA Conjugate Control Zhao XC1, Berg BG, Chem Senses. 2009 Jun;34(5):363-72. doi: 10.1093/chemse/bjp013. Epub 2009 Mar 20. "Morphological and physiological characteristics of the serotonin-immunoreactive neuron in the antennal lobe of the male oriental tobacco budworm, Helicoverpa assulta" Moth IHC 19304764
20081 5-HT (Serotonin) / BSA Conjugate Control Iwano, Masaaki, et al. "Neurons associated with the flip?flop activity in the lateral accessory lobe and ventral protocerebrum of the silkworm moth brain." Journal of Comparative Neurology 518.3 (2010): 366-388. Moth IHC 22 19950256
20081 5-HT (Serotonin) / BSA Conjugate Control Fujimoto, Kazuki, et al. "Sensory and autonomic innervation of the cervical intervertebral disc in rats: the pathomechanics of chronic discogenic neck pain." Spine 37.16 (2012): 1357-1362. Rat 6 22310098

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MSDS

This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.

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