Pre-adsorption of Serotonin antisera, diluted according to the antibody specification sheet, with 20 µg/ml Serotonin/BSA conjugate following the instructions below provides complete blockage of Serotonin immunolabeling. The conjugate is provided as 50 µg of lyophilized Serotonin creatinine sulfate coupled to BSA with paraformaldehyde. Also, this antiserum contains 0.09% sodium azide. Please read the instructions carefully before beginning the procedure.
Quantity / Volume: 50 µg
01/21/2013 - 11:45:25 AM
Good for pre-adsorbing rabbit anti-5HT antibody (#20080)
____Incubate 40mg/ml 5HT-BSA conjugate with 1:4,000, 1:10,000, 1:20,000, 1:40,000 and 1:80,000 dilutions of the rabbit anti-serotonin antibody overnight at room temperature with mild agitation. In parallel, equal volumes of these dilutions in which the 5HT-BSA conjugate is omitted are also incubated under the same conditions to provide positive controls.
_____All samples are spun down at 14,000RPM for 15 minutes at 4oC
_____Supernatant is collected for use in standard 5HT labeling protocol.
West Virginia University
|PROD #||ANTIBODY||CITATION||SPECIES REACTIVITY||APPL METHOD||RESEARCH AREA||# CITED BY||PUBMED ID|
|20081||5-HT (Serotonin) / BSA Conjugate Control||De Girolamo, P., et al. "Co?localization of Trk neurotrophin receptors and regulatory peptides in the endocrine cells of the teleostean stomach." The Anatomical Record 256.3 (1999): 219-226.||Sparus Aurata (Fish), Scorpaena Porcus (Fish)||IHC||26||10521780|
|20081||5-HT (Serotonin) / BSA Conjugate Control||J Comp Neurol. 2008 Dec 20;511(6):804-31. doi: 10.1002/cne.21857. Development of the serotoninergic system in the central nervous system of a shark, the lesser spotted dogfish Scyliorhinus canicula. Carrera I1, Molist P, Anadón R, Rodríguez-Moldes I.||Fish||IF||18925650|
|20081||5-HT (Serotonin) / BSA Conjugate Control||Delgado, Nadia, Deborah Vallejo, and Mark W. Miller. "Localization of serotonin in the nervous system of Biomphalaria glabrata, an intermediate host for schistosomiasis." Journal of Comparative Neurology 520.14 (2012): 3236-3255.||Snail||0||22434538|
|20081||5-HT (Serotonin) / BSA Conjugate Control||Zhao XC1, Berg BG, Chem Senses. 2009 Jun;34(5):363-72. doi: 10.1093/chemse/bjp013. Epub 2009 Mar 20. "Morphological and physiological characteristics of the serotonin-immunoreactive neuron in the antennal lobe of the male oriental tobacco budworm, Helicoverpa assulta"||Moth||IHC||19304764|
|20081||5-HT (Serotonin) / BSA Conjugate Control||Iwano, Masaaki, et al. "Neurons associated with the flip?flop activity in the lateral accessory lobe and ventral protocerebrum of the silkworm moth brain." Journal of Comparative Neurology 518.3 (2010): 366-388.||Moth||IHC||22||19950256|
|20081||5-HT (Serotonin) / BSA Conjugate Control||Fujimoto, Kazuki, et al. "Sensory and autonomic innervation of the cervical intervertebral disc in rats: the pathomechanics of chronic discogenic neck pain." Spine 37.16 (2012): 1357-1362.||Rat||6||22310098|
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Q & A Wall
This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.Download MSDS