VAT (Vesicular Acetylcholine Transporter) Antibody

VAT (Vesicular Acetylcholine Transporter) Antibody
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Price: $400.00
Product ID : 24286

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Description

The antibody produces strong labeling of VAChT at a dilution of 1/3,000 - 1/5,000 using biotin-streptavidin/HRP technique in rat basal forebrain. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.

Immunolabeling is completely abolished by pre-adsorption with synthetic rat VAChT (511-530). Immunolabeling of transfected cells demonstrates no cross reactivity with vesicular monoamine transporters.

Photo Description: IHC image of neurons in the rat basal forebrain staining for VAT. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% rabbit serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, rabbit anti-goat secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. 

Host: Goat

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Species Reactivity: Human, Mouse, Rat

Availability: In Stock

Alternate Names:  Solute carrier family 18, member 3; solute carrier family 18 (vesicular monoamine) member 3; solute carrier family 18 (vesicular acetylcholine), member 3; rVAT; VACht, anti-VAT, anti-VACht 

Gene Symbol / ID, Accession #:  Slc18a3, 60422

RRID:  AB_572269

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Publications

PROD # ANTIBODY CITATION SPECIES REACTIVITY APPL METHOD RESEARCH AREA # CITED BY PUBMED ID
24286 VAChT (Vesicular Acetylcholine Transporter) Antibody J Comp Neurol. 2006 Nov 1;499(1):64-89. Afferent and efferent connections of the rat retrotrapezoid nucleus. Rosin DL1, Chang DA, Guyenet PG. Rat IHC 16958085
24286 VAChT (Vesicular Acetylcholine Transporter) Antibody Wang, Qing-Ping, et al. "An electron microscopic observation of the vesicular acetylcholine transporter-immunoreactive fibers in the rat dorsal raphe nucleus." Brain research bulletin 46.6 (1998): 555-561. Rat 8 9744294
24286 VAChT (Vesicular Acetylcholine Transporter) Antibody Hill, David P., and Kent A. Robertson. "Characterization of the cholinergic neuronal differentiation of the human neuroblastoma cell line LA-N-5 after treatment with retinoic acid." Developmental brain research 102.1 (1997): 53-67. Human IHC 35 9298234
24286 VAChT (Vesicular Acetylcholine Transporter) Antibody Muller, Jay F., Franco Mascagni, and Alexander J. McDonald. "Cholinergic innervation of pyramidal cells and parvalbumin?immunoreactive interneurons in the rat basolateral amygdala." Journal of Comparative Neurology 519.4 (2011): 790-805. Rat ICC 11 21246555
24286 VAChT (Vesicular Acetylcholine Transporter) Antibody J Comp Neurol. 2000 Mar 27;419(1):32-48. Dendritic and axonal targeting of the vesicular acetylcholine transporter to membranous cytoplasmic organelles in laterodorsal and pedunculopontine tegmental nuclei. Garzón M1, Pickel VM. Rat ICC 24 10717638
24286 VAChT (Vesicular Acetylcholine Transporter) Antibody Fregoso, S. P., and D. B. Hoover. "Development of cardiac parasympathetic neurons, glial cells, and regional cholinergic innervation of the mouse heart." Neuroscience 221 (2012): 28-36. Mouse 2 22766236
24286 VAChT (Vesicular Acetylcholine Transporter) Antibody SHAO, LIFANG, et al. "Differential effects of traumatic brain injury on vesicular acetylcholine transporter and M2 muscarinic receptor mRNA and protein in rat." Journal of neurotrauma 16.7 (1999): 555-566. Rat 28 10447068
24286 VAChT (Vesicular Acetylcholine Transporter) Antibody Hill, David P., and Kent A. Robertson. "Differentiation of LA-N-5 neuroblastoma cells into cholinergic neurons: methods for differentiation, immunohistochemistry and reporter gene introduction." Brain Research Protocols 2.3 (1998): 183-190. IHC 18 9507116
24286 VAChT (Vesicular Acetylcholine Transporter) Antibody Torres-Reveron, Annelyn, et al. "Early postnatal exposure to methylphenidate alters stress reactivity and increases hippocampal ectopic granule cells in adult rats." Brain research bulletin 78.4 (2009): 175-181. Rat ICC 3 19100815
24286 VAChT (Vesicular Acetylcholine Transporter) Antibody Hoover, Jeffrey L., et al. "Effect of neurturin deficiency on cholinergic and catecholaminergic innervation of the murine eye." Experimental eye research 122 (2014): 32-39. Mouse 0 24657391
24286 VAChT (Vesicular Acetylcholine Transporter) Antibody Wotus, Cheryl, et al. "Forced desynchrony reveals independent contributions of suprachiasmatic oscillators to the daily plasma corticosterone rhythm in male rats." PloS one 8.7 (2013): e68793. Rat 4 23894346
24286 VAChT (Vesicular Acetylcholine Transporter) Antibody Gray, Jason D., et al. "Methylphenidate administration to juvenile rats alters brain areas involved in cognition, motivated behaviors, appetite, and stress." The Journal of neuroscience 27.27 (2007): 7196-7207. Rat ICC 62 17611273
24286 VAChT (Vesicular Acetylcholine Transporter) Antibody Muller, Jay F., et al. "Muscarinic cholinergic receptor M1 in the rat basolateral amygdala: Ultrastructural localization and synaptic relationships to cholinergic axons." Journal of Comparative Neurology 521.8 (2013): 1743-1759. Rat 1 23559406
24286 VAChT (Vesicular Acetylcholine Transporter) Antibody Pickel, Virginia M., et al. "Neurokinin 1 receptor distribution in cholinergic neurons and targets of substance P terminals in the rat nucleus accumbens." Journal of Comparative Neurology 423.3 (2000): 500-511. Rat 30 10870089
24286 VAChT (Vesicular Acetylcholine Transporter) Antibody Ferbinteanu, Janina, and Matthew L. Shapiro. "Prospective and retrospective memory coding in the hippocampus." Neuron 40.6 (2003): 1227-1239. Rat IHC 270 14687555
24286 VAChT (Vesicular Acetylcholine Transporter) Antibody Hempfling, C., W. L. Neuhuber, and J. W, Serotonin-immunoreactive neurons and mast cells in the mouse esophagus suggest involvement of serotonin in both motility control and neuroimmune … 8 not found
24286 VAChT (Vesicular Acetylcholine Transporter) Antibody Towart, Laura A., et al. "Subcellular relationships between cholinergic terminals and estrogen receptor?? in the dorsal hippocampus." Journal of Comparative Neurology 463.4 (2003): 390-401. Rat 81 12836175
24286 VAChT (Vesicular Acetylcholine Transporter) Antibody Kaplan, Theodore J., et al. "Ultrastructural evidence for mu-opioid modulation of cholinergic pathways in rat dentate gyrus." Brain research 1019.1 (2004): 28-38. Rat IHC 11 15306235
24286 VAChT (Vesicular Acetylcholine Transporter) Antibody Oda, Satoko, et al. "Ultrastructure of ascending cholinergic terminals in the anteroventral thalamic nucleus of the rat: a comparison with the mammillothalamic terminals." Brain research bulletin 59.6 (2003): 473-483. Rat ICC 11 12576145
24286 VAChT (Vesicular Acetylcholine Transporter) Antibody Yao, Weiping, and Donald A. Godfrey. "Vesicular acetylcholine transporter in the rat cochlear nucleus: an immunohistochemical study." Journal of Histochemistry & Cytochemistry 47.1 (1999): 83-90. Rat IHC 26 9857215
24286 VAChT (Vesicular Acetylcholine Transporter) Antibody Svingos, Adena L., Eric EO Colago, and Virginia M. Pickel. "Vesicular acetylcholine transporter in the rat nucleus accumbens shell: Subcellular distribution and association with ??opioid receptors." Synapse 40.3 (2001): 184-192. Rat 15 11304756
24286 VAChT (Vesicular Acetylcholine Transporter) Antibody Langdale, C. L., et al. "Maintenance of bladder innervation in diabetes: a stereological study of streptozotocin-treated female rats." Autonomic Neuroscience 185 (2014): 59-66. Rat 2 not defined

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MSDS

This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.

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