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The antibody has a proven strong biotin-streptavidin/HRP staining at a 1/2000 - 1/4000 dilution in rat hypothalamus.
Staining is completely eliminated by pretreatment of the diluted antibody with 10 µg/mL of arginine vasopressin. Pre-adsorption with as much as 100 µg/mL of oxytocin had no effect on immunolabeling.
Photo Description: IHC image of rat hypothalamus staining for vasopressin. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
Quantity / Volume: 100 µL
State: Lyophilized Whole Serum
Reacts With: Anole, Bluehead Wrasses (Fish), Chicken, Clarias Batrachus (Catfish), Cockroach, Ewe / Sheep, Hamster, Human, Hydra Vulgaris (Polyp), Locust, Mole Rat, Monkey, Mouse, Pigeon, Rat, Shrew, Squirrel
Availability: In Stock
Alternate Names: Vasopressin (Arg); Antidiuretic Hormone; Arginine vasopressin; Vasopressin-neurophysin 2-copeptin; ARG-vasopressin; prepro-AVP-NPII; neurohypophyseal; vasopressin-neurophysin II-copeptin; prepro-arginine-vasopressin-neurophysin II; AVP-NPII; VP; ADH; ARVP; AVRP, anti-vasopressin
Gene Symbol: AVP
Entrez Gene: 396101 Chicken
Entrez Gene: 443477 Ewe / Sheep
Entrez Gene: 551 Human
Entrez Gene: 101717720 Mole Rat
Entrez Gene: 717101 Monkey
Entrez Gene: 11998 Mouse
Entrez Gene: 24221 Rat
Entrez Gene: 102870182 Shrew
12/09/2015 - 10:25:52 AM
Excellent for IHC
12/18/2012 - 01:11:07 PM
We use a total 1:5000 concentration. It is mixed with 10% serum, 10% biotin diluted in 80% PBS-T and incubated for 48-72 hours at 4 degrees C. We used brain tissue from Thamnophis sirtalis (Red-sided garter snake).
Portland State University
Department of Biology
Portland, OR 97207
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Q & A Wall
This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.Download MSDS