Somatostatin Antibody

Somatostatin Antibody
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Price: $275.00
Product ID : 20067

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Description

The antibody has a proven strong Biotin-Streptavidin/HRP staining at a 1/1000-1/2000 dilution in rat hypothalamus (median eminence). The specificity of the antiserum was examined by soluble pre-adsorption with the peptides in question at a final concentration of 106M.

Somatostatin immunolabeling was completely abolished by pre-adsorption with somatostatin, somatostatin 25, and somatostatin 28. Pre-adsorption with the following peptides resulted in no reduction of immunostaining: substance P, amylin, glucagon, insulin, neuropeptide Y, and VIP.

Photo Description: IHC image of the median eminence staining for somatostatin (above). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. Florescent secondary (below).

Somatostatin median eminence #20067

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Reacts With: Alligator, Anuran (Frog), Bat, Bird, Buffalo (Bubalus Bubalis), Bullfrog, Cat, Chick, Chicken, Dermophis Mexicanus Mexican Caecilian  (Amphibian ), Dog, Duck, Fish, Fowl, Frog (Xenopus Laevis), Guinea Pig, Hamster, Human, Lizard, Malayan Pangolin (Sunda Pangolin), Manducta Sexta (Moth), Monkey, Mouse, Pig, Pigeon, Quail, Rabbit, Raccoon, Rana Pipiens (Leopard Frog), Rat, Scorpaena Porcus (Fish), Sea Bass (Fish), Sheep, Snail, Snake, Sparus Aurata (Fish), Starfish, Sting Ray, Turtle, Zebrafish

Availability: In Stock

Preabsorption Control: Available at Sigma Aldrich, Catalog # S9129

Alternate Names:  Growth hormone release-inhibiting factor; Somatostatin 14; Somatostatin 28; prepro-somatostatin; SMST; anti-somatostatin

Gene Symbol:  SST

RRID:  AB_572264

Database Links:
  Entrez Gene: 102569049     Alligator
  Entrez Gene: 102242181     Bat
  Entrez Gene: 100219295     Bird
  Entrez Gene: 102394725     Buffalo
  Entrez Gene: 101096911     Cat
  Entrez Gene: 396279           Chicken
  Entrez Gene: 403993           Dog
  Entrez Gene: 101800536      Duck
  Entrez Gene: 100037904      Frog
  Entrez Gene: 100730851      Guinea Pig
  Entrez Gene: 100774243      Hamster
  Entrez Gene: 6750               Human

  Entrez Gene: 708626           Monkey
  Entrez Gene: 20604             Mouse
  Entrez Gene: 494469           Pig

  Entrez Gene: 102092386      Pigeon
  Entrez Gene: 107318299      Quail
  Entrez Gene: 100353697      Rabbit
  Entrez Gene: 24797             Rat
  Entrez Gene: 443006           Sheep
  Entrez Gene: 102451183      Turtle
  Entrez Gene: 79186             Zebrafish

Reviews

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Reviewed by Dan_Tylee
04/24/2012 - 09:32:00 AM
Strong, Cell-Specific Labeling

Tissue: Maccaca fasciscularis, perfused with saline and 4% paraformaldehyde, dehydrated through increase sucrose gradients, sectioned at 40 um.

Method: Immunocytochemistry on free floating sections. Incubated with ImmunoStar's primary antibody at a concentration of 1:1000 for 4 days in 10% normal goat serum (in 0.1M PO4 buffer with .3% Triton X-100). Rinsed, then incubated with Vector Lab's biotinylated goat anti-rabbit secondary antibody (#BA-1000) for 40 minutes at room temperature in the same 10% normal goat serum solution. Rinsed, then incubated with Vector's standard peroxidase kit (#PK-4000) for 60 minutes at room temperature in 0.1M PO4 buffer with 0.3% Triton X-100. Rinsed, then developed using the instructions for the kit.

Results: Strong, cell-specific labeling. Crisp, punctuate cell bodies. Low background signal. Extremely helpful for delineating the boundaries of the lateral core of the bed nucleus of the stria terminalis (BNST).


Daniel Tylee
Department of Neurobiology & Anatomy
University of Rochester - School of Medicine and Dentistry

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MSDS

This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.

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