Somatostatin 28 Antibody

Somatostatin 28 Antibody
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Price: $275.00
Product ID : 20089

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Description

The antibody has a proven Biotin-Streptavidin/HRP staining at a 1/1000-1/2000 dilution in rat hypothalamus/median eminence.

Staining is completely eliminated by pretreatment of the diluted antibody with 100 µg/mL of Somatostatin 28 (Tyr1-14).

Photo Description: IHC image of the median eminence staining for somatostatin-28 with standard biotin-streptavidin/HRP and DAB chromogen (above) and FITC-conjugated secondary antibody (below). The tissue was fixed with 4% formaldehyde/0.05% glutaraldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:2000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS.

Somatostatin 28 #20089

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Reacts With: Cat, Guinea Pig, Human, Mouse, Rat

Availability: In Stock

Alternate Names:   Growth hormone release-inhibiting factor 28; SMST; somatostatin, (3C11 in reference # 2345961), anti-somatostatin 28

Gene Symbol / ID, Accession #:   SST,6750

 RRID:  AB_572265

Reviews

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Reviewed by isaacson
06/05/2013 - 02:52:50 PM
SOM 28 antibody

I tested the SOM 28 antibody on 50um piriform cortex sections from mice fix-perfused but NOT cryopreserved. Staining and signal to noise were very good using a 1:500 dilution of the antibody in combination iwth 5% normal goat serum and 0.3% TX100 in PBS and O/N incubation at 4%. Immunostaining was visualized using a 1:500 GAR-Alexa 488 secondary anbibody.

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MSDS

This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.

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