The antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat thalamus, cortex, and hippocampus.
The antiserum has been characterized as specific to parvalbumin; please see reference listed below. Recommended primary dilution is 1/5,000 - 1/8,000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique.
Photo Description: IHC image of the rat hippocampus staining for parvalbumin. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-guinea pig secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
The second image above entitled "D." is parvalbumin (RED) interneurons pictured in mouse striatum, GREEN is GFP expressed by medium spiny neurons, provided Alipi Naydenov, Neurobiology and Behavior, Stella Lab University of Washington (see review below).
Host: Guinea Pig
Quantity / Volume: 100 µL
State: Lyophilized Whole Serum
Reacts With: Rat
Availability: In Stock
Alternate Names: Parvalbumin alpha; Pva; Parvalbumin (calcium binding protein); PALB1, PC255L (Millipore Cat #), anti-parvalbumin
Gene Symbol / ID, Accession #: Pvalb, 2526
11/15/2012 - 02:23:41 PM
Antibody Performed Very Well
Immunohistochemistry: Mice were euthanized and perfused with PFA (4% in PBS), post fixed for 24 hrs, and their brains cryoprotected in 15% sucrose (24 hrs) followed by 30% sucrose (48 hrs). Coronal sections that included the corticostriatal, globus pallidus, or substantia nigra regions (30 um) were prepared using a microtome and then stored in PBS at 4C until processing. Sections were processed/stained in parallel as follows: Free floating sections were rinsed 3x with PBS and incubated 90 min at room temperature (RT) in PBS supplemented with donkey serum (5%) and Triton X-100 (1%). Primary antibody combinations were prepared as a master stock in PBS supplemented with donkey serum (2.5%) and Triton X-100 (0.5%) and applied to sections for 72 hrs at 4oC with gentle agitation. Sections were then rinsed 8x with PBS supplemented with Tween-20 (0.05%, at RT) and incubated with a secondary antibodies diluted in PBS supplemented with donkey serum (2.5%) and Triton X-100 (0.5%) for 1 hr at RT with gentle agitation, followed by 7 rinses with PBS and one rinse with deionized water. Sections were mounted onto slides and allowed to dry for ~18 hrs, after which cover slips were mounted with Vectashield and sealed with nail polish.
Review submitted by:
Neurobiology and Behavior, Stella Lab
University of Washington
|PROD #||ANTIBODY||CITATION||SPECIES REACTIVITY||APPL METHOD||RESEARCH AREA||# CITED BY||PUBMED ID|
|24428||Parvalbumin Antibody||André V1, Marescaux C, Nehlig A, Fritschy JM., Hippocampus. 2001;11(4): 452-68."Alterations of hippocampal GAbaergic system contribute to development of spontaneous recurrent seizures in the rat lithium-pilocarpine model of temporal lobe epilepsy"||Rat||IHC||135||11530850|
|24428||Parvalbumin Antibody||Ünal B1, Shah F, Kothari J, Tepper JM., Brain Struct Funct. 2015 Jan;220(1):331-49. doi: 10.1007/s00429-013-0658-8. Epub 2013 Oct 31. Anatomical and electrophysiological changes in striatal TH interneurons after loss of the nigrostriatal dopaminergic pathway.||2||24173616|
|24428||Parvalbumin Antibody||Laverghetta AV1, Toledo CA, Veenman CL, Yamamoto K, Wang H, Reiner A, "Brain Behav Evol. 2006;67(1):10-38. Epub 2005 Oct 10. Cellular localization of AMPA type glutamate receptor subunits in the basal ganglia of pigeons (Columba livia)"||Pigeon||IHC||16219996|
|24428||Parvalbumin Antibody||Bäckman C1, Hoffer BJ, Misawa H, Morales M., Brain Res. 2001 Jun 8;903(1-2):26-32."Cellular mRNA expression of the transcription factor NGFI-B suggests a gene regulatory role in striatal opiate-peptide neurons"||Transcription Factor||10||11382384|
|24428||Parvalbumin Antibody||Weiss, John H., et al. "Cortical neurons containing somatostatin?or parvalbumin?like immunoreactivity are atypically vulnerable to excitotoxic injury in vitro." Neurology 40.8 (1990): 1288-1288.||71||1974332|
|24428||Parvalbumin Antibody||Kuang, Yi, et al. "Dicer1 and MiR-9 are required for proper Notch1 signaling and the Bergmann glial phenotype in the developing mouse cerebellum." Glia 60.11 (2012): 1734-1746.||Mouse||IF||2||22836445|
|24428||Parvalbumin Antibody||Deng, Y. P., et al. "Differential localization of the GluR1 and GluR2 subunits of the AMPA-type glutamate receptor among striatal neuron types in rats." Journal of chemical neuroanatomy 33.4 (2007): 167-192.||Rat||IF||27||17446041|
|24428||Parvalbumin Antibody||Anat Rec (Hoboken). 2010 Jun;293(6): 1024-32. doi: 10.1002/ar.21116. Distribution analysis of deacetylase SIRT1 in rodent and human nervous systems. Zakhary SM1, Ayubcha D, Dileo JN, Jose R, Leheste JR, Horowitz JM, Torres G.||Human, Mouse, Rat||IHC||20225204|
|24428||Parvalbumin Antibody||Horne, Eric A., et al. "Downregulation of cannabinoid receptor 1 from neuropeptide Y interneurons in the basal ganglia of patients with Huntington's disease and mouse models." European Journal of Neuroscience 37.3 (2013): 429-440.||Mouse, Human||huntington||4||23167744|
|24428||Parvalbumin Antibody||Arabadzisz, Dimitrula, et al. "Epileptogenesis and chronic seizures in a mouse model of temporal lobe epilepsy are associated with distinct EEG patterns and selective neurochemical alterations in the contralateral hippocampus." Experimental neurology 194.1 (2005): 76-90.||Mouse||57||15899245|
|24428||Parvalbumin Antibody||J Comp Neurol. 2006 Dec 1;499(4):603-12. Estradiol-induced modulation of estrogen receptor-beta and GABA within the adult neocortex: a potential transsynaptic mechanism for estrogen modulation of BDNF. Blurton-Jones M1, Tuszynski MH.||Rat||IF||32||17029253|
|24428||Parvalbumin Antibody||Sarabi, Arezou, et al. "GFR-1 is expressed in parvalbumin GABAergic neurons in the hippocampus." Brain research 877.2 (2000): 262-270.||20||10986340|
|24428||Parvalbumin Antibody||Sarabi, Arezou, et al. "GFR-1 mRNA in dopaminergic and nondopaminergic neurons in the substantia nigra and ventral tegmental area." Journal of Comparative Neurology 441.2 (2001): 106-117.||Rat||57||11745638|
|24428||Parvalbumin Antibody||Murakami, K., Y. Ishikawa, and F. Sato. "Localization of ?7 nicotinic acetylcholine receptor immunoreactivity on GABAergic interneurons in layers I||IF||0||23985568|
|24428||Parvalbumin Antibody||Briatore, Federica, et al. "Quantitative organization of GABAergic synapses in the molecular layer of the mouse cerebellar cortex." PloS one 5.8 (2010): e12119.||Mouse||IHC||16||20711348|
|24428||Parvalbumin Antibody||Schneider Gasser, Edith M., et al. "Reorganization of GABAergic circuits maintains GABAA receptor?mediated transmission onto CA1 interneurons in ?1?subunit?null mice." European Journal of Neuroscience 25.11 (2007): 3287-3304.||Mouse||24||17552997|
|24428||Parvalbumin Antibody||Browning, Philip GF, et al. "Severe scene learning impairment, but intact recognition memory, after cholinergic depletion of inferotemporal cortex followed by fornix transection." Cerebral Cortex 20.2 (2010): 282-293.||Monkey||17||19447862|
|24428||Parvalbumin Antibody||Kleijer, K. T. E., et al. "Contactin-5 expression during development and wiring of the thalamocortical system." Neuroscience 310 (2015): 106-113.||0||not defined|
Products You May Like
Q & A Wall
I want to know if this antibody could be used to perform western blot and your recommend dilution.
Question By Shenyue Q
No answer found for this question.
I have a question concerning PV antibody Product ID 24428. I would like to use it to detect parvalbumin in primary mouse neurons. Is it possible?
Question By Agnieszka S
No answer found for this question.
This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.Download MSDS