The ImmunoStar Oxytocin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/4,000 - 1/8,000 in PBS/0.3% Triton X-100 - Bn/Av-HRP.
Staining is completely eliminated by pretreatment of 1 mL of the diluted antibody with 5 µg of Oxytocin. Pretreatment of 1 mL of the diluted antibody with as much as 100 µg of vasopressin does not diminish staining.
Photo Description: IHC image of neurons staining for oxytocin in the rat hypothalamus (above) and higher magnification of the rat hypothalamus with nickel preparation (below). The tissue was fixed with 4% formaldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
Quantity / Volume: 100 µL
State: Lyophilized Whole Serum
Reacts With: Bat, Bovine, Chicken, Dog, Frog (Xenopus Laevis), Guinea Pig, Hamster, Human, Manducta Sexta (Moth), Melopsittacus Undulatus (Bird), Mole Rat, Monkey, Mouse, Rat, Sheep, Vole
Availability: In Stock
Alternate Names: Oxytocin-neurophysin 1; Ocytocin; neurophysin I; oxytocin, prepropeptide; oxytocin, prepro- (neurophysin I); oxytocin-neurophysin I, preproprotein; OT; OT-NPI; OXT-NPI; oxytocin/neurophysin I prepropeptide, anti-Oxytocin
Gene Symbol / ID, Accession #: OXT, 5020
04/07/2016 - 03:03:57 PM
Excellent for IF
06/12/2015 - 11:45:56 AM
09/13/2013 - 03:03:21 PM
Not useful for experiments in nonmammalian species
Published work in birds (Cantwell and Cassone; PubMed ID: 1699890; cited on the antibody product page) demonstrates that preadsorption with mesotocin does in fact eliminate immunolabeling in birds. However, those authors did not conduct preadsorptions with the closely related neuropeptide vasotocin, which differs from mesotocin by only one amino acid. Vasotocin is the nonmammalian homologue of vasopressin and is the ancestral peptide for all vertebrate peptides of the oxytocin-vasopressin family. Importantly, vasotocin and mesotocin are expressed in separate subpopulations of neurons in the hypothalamus (e.g., separate subpopulations in the paraventricular nucleus), as are vasopressin and oxytocin, but the oxytocin antibody 20068 labels both, as we have discovered in pilot experiments using a selective vasotocin antibody alongside the oxytocin 20068. The oxytocin antibody also labels extrahypothalamic neurons in the bed nucleus of the stria terminalis that are known to express only vasotocin, not mesotocin (see Goodson et al., 2012, PNAS, 109 supp. 1, 10685-10692).
Thus, although the oxytocin antibody 20068 does recognize nonmammalian forms of oxytocin, it is not useful for experiments in nonmammalian species due to its cross-reactivity to vasotocin, a peptide found in all nonmammalian vertebrates.
Jim Goodson, Professor
Evolution, Ecology and Behavior
Department of Biology
08/26/2013 - 10:29:26 AM
Oxytocin DAB Staining in Rat
The cell bodies are really easy to see at low magnification throughout the brain. The fibers are hard to see outside of the PVN at anything less than 40x in oil.
Neurodevelopmental Biology Laboratory
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This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.Download MSDS