Oxytocin Antibody

Oxytocin Antibody
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Price: $275.00
Product ID : 20068

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Description

The ImmunoStar Oxytocin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/4,000 - 1/8,000 in PBS/0.3% Triton X-100 - Bn/Av-HRP.

Staining is completely eliminated by pretreatment of 1 mL of the diluted antibody with 5 µg of Oxytocin. Pretreatment of 1 mL of the diluted antibody with as much as 100 µg of vasopressin does not diminish staining.

Photo Description:  IHC image of neurons staining for oxytocin in the rat hypothalamus (above) and higher magnification of the rat hypothalamus with nickel preparation (below). The tissue was fixed with 4% formaldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

Oxytocin hypothalamus #20068

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Reacts With: Bat, Bovine, Chicken, Dog, Frog (Xenopus Laevis), Guinea Pig, Hamster, Human, Manducta Sexta (Moth), Melopsittacus Undulatus (Bird), Mole Rat, Monkey, Mouse, Rat, Sheep, Vole

Availability: In Stock

Alternate Names:  Oxytocin-neurophysin 1; Ocytocin; neurophysin I; oxytocin, prepropeptide; oxytocin, prepro- (neurophysin I); oxytocin-neurophysin I, preproprotein; OT; OT-NPI; OXT-NPI; oxytocin/neurophysin I prepropeptide, anti-Oxytocin

Gene Symbol / ID, Accession #:  OXT, 5020

RRID:  AB_572258

Reviews

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Reviewed by SBUFFINGTON
04/07/2016 - 03:03:57 PM
Excellent for IF

Excellent signal in mouse hypothalamus when used at 1:2000. Brains were first perfused with PBS and then with 4% PFA followed by overnight drop-fixation in 4% PFA. 30um-thick slices were cut on a sliding blade microtome and then immunostained as floating sections. Slices were incubated in the primary anti-oxytocin antibody overnight rocking at 4oC. Primary antibodies were visualized with anti-rabbit secondaries, incubation for 1h at RT the following day.

Reviewed by banerjeelab
06/12/2015 - 11:45:56 AM
Would recommend.

This antibody worked very well at 1:2,000 in PFA-fixed, cryosectioned mouse brain tissue. OXT-positive cells in the PVN were very clearly labeled with minimal background.

Reviewed by user
09/13/2013 - 03:03:21 PM
Not useful for experiments in nonmammalian species

As stated on the antibody product page, this antibody exhibits reactivity to forms of oxytocin across a wide range of species, including species that express Ile8-oxytocin (mesotocin), the oxytocin form found in most marsupials, birds, reptiles and amphibians.

Published work in birds (Cantwell and Cassone; PubMed ID: 1699890; cited on the antibody product page) demonstrates that preadsorption with mesotocin does in fact eliminate immunolabeling in birds. However, those authors did not conduct preadsorptions with the closely related neuropeptide vasotocin, which differs from mesotocin by only one amino acid. Vasotocin is the nonmammalian homologue of vasopressin and is the ancestral peptide for all vertebrate peptides of the oxytocin-vasopressin family. Importantly, vasotocin and mesotocin are expressed in separate subpopulations of neurons in the hypothalamus (e.g., separate subpopulations in the paraventricular nucleus), as are vasopressin and oxytocin, but the oxytocin antibody 20068 labels both, as we have discovered in pilot experiments using a selective vasotocin antibody alongside the oxytocin 20068. The oxytocin antibody also labels extrahypothalamic neurons in the bed nucleus of the stria terminalis that are known to express only vasotocin, not mesotocin (see Goodson et al., 2012, PNAS, 109 supp. 1, 10685-10692).

Thus, although the oxytocin antibody 20068 does recognize nonmammalian forms of oxytocin, it is not useful for experiments in nonmammalian species due to its cross-reactivity to vasotocin, a peptide found in all nonmammalian vertebrates.

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Jim Goodson, Professor
Evolution, Ecology and Behavior
Department of Biology
Indiana University

Reviewed by ietminah@gmail.com
08/26/2013 - 10:29:26 AM
Oxytocin DAB Staining in Rat

I used the Antibody at 1:4000 in rat.

The cell bodies are really easy to see at low magnification throughout the brain. The fibers are hard to see outside of the PVN at anything less than 40x in oil.


Reviewed by:

Yaminah Gilles
Howard University
Neurodevelopmental Biology Laboratory

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MSDS

This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.

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