Opioid Receptor-Mu (MOR) Antibody

Opioid Receptor-Mu (MOR) Antibody
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Price: $375.00
Product ID : 24216

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Description

The ImmunoStar Mu Opioid Receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat caudate putamen and spinal cord (dorsal horn) using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/6000 - 1/10000 in PBS/0.3% Triton X-100 - Biotin/avidin-HRP Technique.

Preadsorption with MOR peptide (384-398) at 10 µg/ml completely eliminates labeling. The specificity of the antiserum was determined by immunolabeling of transfected cells, Western Blot analysis and immunoisolation studies.

Photo Description: IHC image of mu opioid receptor staining in the rat striatum. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:10000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Reacts With:  Cat, Chicken, Frog, Guinea Pig, Human, Monkey, Mouse, Rat, Starling, Tadpole

Availability: In Stock

Alternate Names:  MOR-1; Mu-type opioid receptor; M-OR1; Mu opiate receptor; Opioid receptor B; MUOR1; M-OR-1; MORA; oprm; opioid receptor, mu 1, anti-MOR

Gene Symbol / ID, Accession #:  Oprm1, 25601

RRID:  AB_572251

Reviews

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Reviewed by marvizon
12/09/2015 - 07:21:16 PM
MOR antibody 24216

This is an excellent antibody to label MORs in the rat spinal cord using immunofluorescence. I have used it to study MOR internalization induced by opioid release in the following papers:
Chen W, Marvizon JC (2009) Acute inflammation induces segmental, bilateral, supraspinally mediated opioid release in the rat spinal cord, as measured by µ-opioid receptor internalization. Neuroscience 161:157-172.
Chen W, Song B, Lao L, Perez OA, Kim W, Marvizon JCG (2007) Comparing analgesia and µ-opioid receptor internalization produced by intrathecal enkephalin: Requirement for peptidase inhibition. Neuropharmacology 53:664-667.
Chen W, Song B, Marvizon JC (2008a) Inhibition of opioid release in the rat spinal cord by a2C adrenergic receptors. Neuropharmacology 54:944-953.
Chen W, Song B, Zhang G, Marvizon JC (2008b) Effects of veratridine and high potassium on µ-opioid receptor internalization in the rat spinal cord: stimulation of opioid release versus inhibition of internalization. J Neurosci Methods 170:285-293.
Marvizon JC, Grady EF, Waszak-McGee J, Mayer EA (1999) Internalization of m-opioid receptors in rat spinal cord slices. Neuroreport 10:2329-2334.
Song B, Chen W, Marvizon JC (2007) Inhibition of opioid release in the rat spinal cord by serotonin 5-HT1A receptors. Brain Res 1158:57-62.
Song B, Marvizon JC (2003a) Peptidases prevent m-opioid receptor internalization in dorsal horn neurons by endogenously released opioids. J Neurosci 23:1847-1858.
Song B, Marvizon JCG (2003b) Dorsal horn neurons firing at high frequency, but not primary afferents, release opioid peptides that produce m-opioid receptor internalization in the rat spinal cord. J Neurosci 23:9171-9184.
Song B, Marvizon JCG (2005) NMDA receptors and large conductance calcium-sensitive potassium channels inhibit the release of opioid peptides that induce m-opioid receptor internalization in the rat spinal cord. Neuroscience 136:549-562.
Walwyn W, Chen W, Kim H, Minasyan A, Ennes H, McRoberts JA, Marvizon JC (2016) Sustained suppression of hyperalgesia during latent sensitization by µ, δ and κ opioid receptors and α2A adrenergic receptors - role of constitutive activity. J Neurosci (in press).

I have tried other MOR antibodies but this is by far the best.

Q & A Wall

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I pretend to separate cellular membranes from citoplasm and make WB by separate in order to semiquantify receptors in membranes and in citoplasm, because I assume that if receptors are in citoplams is because they were endocyted after being activated. So, is this assumtion true?
Question By Eli

We currently do not carry an antibody to the phosphorylated mu opioid receptor. Also, it is best practice to run an antibody on both the membrane and cytosolic fractions to get an idea of the antigen's expression in your experimental conditions.

Answer By Andy K. on 2015-11-03 11:35:59

We are using Mu Opioid Receptor antibody (#24216) for Western Blotting and need to know with what species it might cross react. In addition, does it detect other receptors (such as delta or kappa)?
Question By Nancy

Our MOR-1 aby is raised against the sequence 384-398 of the cloned rat MOR-1. The sequence is 100% homolgous to rat, pig, mouse, human and macac (monkey). The MW based on amino acids of rat MOR-1 is around 44-45 kD, but the protein is heavily glycosylated. Another researcher used our aby and saw around 50-52 kD. The antibody was characterized as specific to MOR-1 by Bob Elde, U of M and does not x-react with delta or kappa. We are sure about the MOR splice variants.


Answer By Jodi on 2015-03-04 11:57:58

I have purchased antibody for the Mu Opioid receptor, Cat# 24216. You present storage recommendations with dilution in PBS or TBS, no higher than 1:10. I have 2 questions; 1: Could we not reconstitute the the vial in 100 ul of PBS instead of dH2O to prevent further dilutions of the antibody? 2: I am assuming that a dilution no higher than 1:10 means that I could reconstitute in 100 ul dH2O and then add 100 ul of PBS?
Question By Nigel

1. We do not have any supporting data for reconstituting the Mu Opioid receptor directly with PBS, so we are not certain if that would cause any problems with the antibody. You could, however, reconstitute it with 100 uL of dH2O and then dilute it with another 100 uL of double strength PBS. This would then leave you with the correct concentration of the PBS and not dilute out the antibody.

2. Yes, you could do a 1:2 dilution of the antibody for storage.

Answer By Jodi on 2005-10-24 11:47:41

MSDS

This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.

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