The ImmunoStar N-terminal neuronal nitric oxide synthase antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus, striatum, cortex and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/1,000 - 1/2,000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP.
By Western blot analysis of brain homogenates the antibody specifically labels a band of approximately 155 kD. Immunolabeling is completely abolished by pre-adsorption with synthetic human nNOS (134-148) at 5 µg per mL of diluted antibody. No cross reactivity with other forms of NOS was observed.
Photo Description: IHC image of neurons staining for the N-terminal of nNOS in the rat cortex (above and below left) and the rat striatum (below right). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:2000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen, bottom left image with nickel stain.
Quantity / Volume: 100 µL
State: Lyophilized Whole Serum
Reacts With: Mouse, Rat
Availability: In Stock
Alternate Names: Constitutive NOS; NC-NOS; NOS type I; Neuronal NOS; Nitric Oxide Synthase; bNOS; Nitric oxide synthase-brain; N-NOS; Peptidyl-cysteine S-nitrosylase; IHPS1; NC-NOS; nitric oxide synthase 1 (neuronal), anti-nNOS:N-Terminal
Gene Symbol / ID, Accession #: NOS1, 4842
|PROD #||ANTIBODY||CITATION||SPECIES REACTIVITY||APPL METHOD||RESEARCH AREA||# CITED BY||PUBMED ID|
|24431||nNOS:N-Terminal (neuronal Nitric Oxide Synthase) Antibody||Becaria, Angelica, et al. "Aluminum and copper in drinking water enhance inflammatory or oxidative events specifically in the brain." Journal of Neuroimmunology 176.1 (2006): 16-23.||Mouse||IHC||116||16697052|
|24431||nNOS:N-Terminal (neuronal Nitric Oxide Synthase) Antibody||J Neurosci. 2012 Feb 15;32(7):2324-34. doi: 10.1523/JNEUROSCI.4610-11.2012. Biglycan is an extracellular MuSK binding protein important for synapse stability. Amenta AR1, Creely HE, Mercado ML, Hagiwara H, McKechnie BA, Lechner BE, Rossi SG, Wang Q, Owens RT, Marrero E, Mei L, Hoch W, Young MF, McQuillan DJ, Rotundo RL, Fallon JR.||Mouse||IF||22396407|
|24431||nNOS:N-Terminal (neuronal Nitric Oxide Synthase) Antibody||FASEB J. 2006 Aug;20(10):1724-6. Epub 2006 Jun 28. Biglycan regulates the expression and sarcolemmal localization of dystrobrevin, syntrophin, and nNOS. Mercado ML1, Amenta AR, Hagiwara H, Rafii MS, Lechner BE, Owens RT, McQuillan DJ, Froehner SC, Fallon JR.||Mouse||WB, IF||16807372|
|24431||nNOS:N-Terminal (neuronal Nitric Oxide Synthase) Antibody||Piskuric, Nikol A., Cathy Vollmer, and Colin A. Nurse. "Confocal immunofluorescence study of rat aortic body chemoreceptors and associated neurons in situ and in vitro." Journal of Comparative Neurology 519.5 (2011): 856-873.||Rat||IF||9||21280041|
|24431||nNOS:N-Terminal (neuronal Nitric Oxide Synthase) Antibody||J Comp Neurol. 2009 Mar 1;513(1):21-37. doi: 10.1002/cne.21919. Developmentally regulated and evolutionarily conserved expression of SLITRK1 in brain circuits implicated in Tourette syndrome. Stillman AA1, Krsnik Z, Sun J, Rasin MR, State MW, Sestan N, Louvi A.||Mouse||WB, IF||19105198|
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This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.Download MSDS