The ImmunoStar Neuropeptide Y Y1 Receptor was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat cortex, arcuate and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are 1/500 - 1/1000 in PBS - Bn/Av-HRP detection.
The antibody was characterized by immunohistochemistry and Western blot. Western blot showed one immunoreactive band of 40 kD and a single high molecular weight band, presumably a precursor molecule.
Preincubation of the antibody with an excess of the synthetic peptide blocked staining. Immunohistochemical staining of rat brain correlates well with Northern analysis, in situ hybridization and receptor autoradiography. BlastP database sequence homology searches confirmed that this sequence is unique to rat, mouse and human NPY Y1 receptors.
Photo Description: IHC image of the rat cortex staining for the neuropeptide Y Y1 recptor. The tissue was fixed with 4% formaldehyde/0.05% glutaraldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
Quantity / Volume: 100 µL
Reacts With: Hamster, Human, Mouse, Rat
Availability: In Stock
Alternate Names: Neuropeptide Y receptor 1; NPY receptor 1; NPYR; NPYY1; NPY1-R; Neuropeptide Y receptor type 1; FC5; NPYR1, neuropeptide Y1 receptor, anti-NPYY1
Gene Symbol / ID, Accession #: neuropeptide Y receptor Y1, anti-NPYY1
|PROD #||ANTIBODY||CITATION||SPECIES REACTIVITY||APPL METHOD||RESEARCH AREA||# CITED BY||PUBMED ID|
|24506||Neuropeptide Y Y1 Receptor Antibody||Dubois, Christophe J., et al. "Activation of NPY type 5 receptors induces a long-lasting increase in spontaneous GABA release from cerebellar inhibitory interneurons." Journal of neurophysiology 107.6 (2012): 1655.||Mouse||ICC||2||22190627|
|24506||Neuropeptide Y Y1 Receptor Antibody||Dailey MJ1, Bartness TJ, Brain Res. 2010 Apr 6;1323:94-108. doi: 2010.01.078. Epub 2010 Feb 4."Arcuate nucleus destruction does not block food deprivation-induced increases in food foraging and hoarding"||Hamster||IHC||aging||20138163|
|24506||Neuropeptide Y Y1 Receptor Antibody||Khan, Javed, and Marc Ladanyi. "Classification of Pediatric Tumors Using DNA Microarrays." Expression Profiling of Human Tumors. Humana Press, 2003. 295-304.||DNA||0||not found|
|24506||Neuropeptide Y Y1 Receptor Antibody||Killough, S. A., F. T. Lundy, and C. R. Irwin. "Dental pulp fibroblasts express neuropeptide Y Y1 receptor but not neuropeptide Y." International endodontic journal 43.10 (2010): 835-842.||Human||3||20636351|
|24506||Neuropeptide Y Y1 Receptor Antibody||Mills, Richard H., Richard K. Sohn, and Paul E. Micevych. "Estrogen-induced ?-opioid receptor internalization in the medial preoptic nucleus is mediated via neuropeptide Y-Y1 receptor activation in the arcuate nucleus of female rats." The Journal of neuroscience 24.4 (2004): 947-955.||Rat||ICC||72||14749439|
|24506||Neuropeptide Y Y1 Receptor Antibody||Regul Pept. 2005 Jul 15;129(1-3):119-24. Functional expression of neuropeptide Y receptors in human neuroblastoma cells. Li AJ1, Ritter S.||Human||7||15927706|
|24506||Neuropeptide Y Y1 Receptor Antibody||Endocrinology. 2006 Sep;147(9):4122-31. Epub 2006 May 25.Influence of dehydration on the expression of neuropeptide Y Y1 receptors in hypothalamic magnocellular neurons. Urban JH1, Leitermann RJ, DeJoseph MR, Somponpun SJ, Wolak ML, Sladek CD.||Rat||IF||16728491|
|24506||Neuropeptide Y Y1 Receptor Antibody||Dimitrov, Eugene L., et al. "Involvement of neuropeptide Y Y1 receptors in the regulation of neuroendocrine corticotropin-releasing hormone neuronal activity." Endocrinology 148.8 (2007): 3666-3673.||Rat||IF||27||17463058|
|24506||Neuropeptide Y Y1 Receptor Antibody||Pain. 2007 May;129(1-2):143-54. Epub 2006 Dec 6. Nerve injury-induced tactile allodynia is present in the absence of FOS labeling in retrogradely labeled post-synaptic dorsal column neurons. Zhang ET1, Ossipov MH, Zhang DQ, Lai J, Porreca F.||Rat||IF||17156921|
|24506||Neuropeptide Y Y1 Receptor Antibody||Klenke, Ulrike, Stephanie Constantin, and Susan Wray. "Neuropeptide Y directly inhibits neuronal activity in a subpopulation of gonadotropin-releasing hormone-1 neurons via Y1 receptors." Endocrinology 151.6 (2010): 2736-2746.||Mouse||IF||26||20351316|
|24506||Neuropeptide Y Y1 Receptor Antibody||Hiruma, Hiromi, et al. "Neuropeptide Y inhibits axonal transport of particles in neurites of cultured adult mouse dorsal root ganglion cells." The Journal of physiology 543.1 (2002): 85-97.||Mouse||ICC||7||12181283|
|24506||Neuropeptide Y Y1 Receptor Antibody||Gibbs JL1, Diogenes A, Hargreaves KM, Br J Pharmacol. 2007 Jan;150(1):72-9. Epub 2006 Dec 4. "Neuropeptide Y modulates effects of bradykinin and prostaglandin E2 on trigeminal nociceptors via activation of the Y1 and Y2 receptors"||Rat||IF||17143304|
|24506||Neuropeptide Y Y1 Receptor Antibody||El Karim, I. A., et al. "Neuropeptide Y Y1 receptor in human dental pulp cells of noncarious and carious teeth." International endodontic journal 41.10 (2008): 850-855.||Human||14||18699789|
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This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.Download MSDS