The mGluR1a Antibody is no longer available from ImmunoStar. To purchase this antibody, please refer to PhosphoSolutions.
The antibody has significant biotin-avidin HRP staining at a 1/300 - 1/500 dilution in rat thalamus, hippocampus and cortex. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended. The antibody is specific for the ~125k monomer and the ~250k mGluR1a dimer in Western blots of rat brain extracts. Immunolabeling for mGluR1a is completely abolished by preadsorption with synthetic rat mGluR1a (1116-1130) at 5 µg per mL of diluted antibody. Note: If needed, low levels of glutaraldehyde (0.1 - 0.3%) may be used in conjunction with paraformaldehyde.
Photo Description: IHC image of neurons staining for the mGluR1 alpha in the rat hippocampus (above) and western blot showing specificity of the antibody at 132 kDa (below). For IHC, the tissue was fixed with 4% formaldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:500 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
Quantity / Volume: 100 µL
Reacts With: Human, Mouse, Rat
Alternate Names: mGluR1a; GRM1a; Gprc1a; Metabotropic glutamate receptor 1; mGluR1; G protein coupled receptor family C group 1 member A; glutamate receptor, metabotropic 1, anti-mGluR1a
Gene Symbol / ID, Accession #: Grm1, 24414
|PROD #||ANTIBODY||CITATION||SPECIES REACTIVITY||APPL METHOD||RESEARCH AREA||# CITED BY||PUBMED ID|
|24426||mGluR1 alpha (Metabotropic Glutamate Receptor) Antibody||Losonczy, Attila, et al. "Cell type dependence and variability in the short?term plasticity of EPSCs in identified mouse hippocampal interneurones." The Journal of physiology 542.1 (2002): 193-210.||Mouse||IHC||82||12096061|
|24426||mGluR1 alpha (Metabotropic Glutamate Receptor) Antibody||Tukker, John J., et al. "Cell type-specific tuning of hippocampal interneuron firing during gamma oscillations in vivo." The journal of neuroscience 27.31 (2007): 8184-8189.||Rat||IF||142||17670965|
|24426||mGluR1 alpha (Metabotropic Glutamate Receptor) Antibody||Alvarez, Francisco J., et al. "Differential distribution of metabotropic glutamate receptors 1a, 1b, and 5 in the rat spinal cord." Journal of Comparative Neurology 422.3 (2000): 464-487.||Rat||IHC||115||10861520|
|24426||mGluR1 alpha (Metabotropic Glutamate Receptor) Antibody||Ferraguti, Francesco, Luca Crepaldi, and Ferdinando Nicoletti. "Metabotropic glutamate 1 receptor: current concepts and perspectives." Pharmacological reviews 60.4 (2008): 536-581.||Mouse||97||19112153|
|24426||mGluR1 alpha (Metabotropic Glutamate Receptor) Antibody||Namkoong, Jin, et al. "Metabotropic glutamate receptor 1 and glutamate signaling in human melanoma." Cancer research 67.5 (2007): 2298-2305.||Human||77||17332361|
|24426||mGluR1 alpha (Metabotropic Glutamate Receptor) Antibody||Ferraguti, Francesco, et al. "Metabotropic glutamate receptor 8-expressing nerve terminals target subsets of GABAergic neurons in the hippocampus." The Journal of neuroscience 25.45 (2005): 10520-10536.||Rat||ICC||57||16280590|
|24426||mGluR1 alpha (Metabotropic Glutamate Receptor) Antibody||Karim, Farzana, Chia-Chuan Wang, and Robert W. Gereau. "Metabotropic glutamate receptor subtypes 1 and 5 are activators of extracellular signal-regulated kinase signaling required for inflammatory pain in mice." The Journal of Neuroscience 21.11 (2001): 3771-3779.||Mouse||ICC||302||11356865|
|24426||mGluR1 alpha (Metabotropic Glutamate Receptor) Antibody||Dobi, Alice, et al. "Neural substrates for the distinct effects of presynaptic group III metabotropic glutamate receptors on extinction of contextual fear conditioning in mice." Neuropharmacology 66 (2013): 274-289.||Mouse||ICC||4||22643400|
|24426||mGluR1 alpha (Metabotropic Glutamate Receptor) Antibody||Bhave, G., et al. "Peripheral group I metabotropic glutamate receptors modulate nociception in mice." Nature neuroscience 4.4 (2001): 417-423.||Mouse||IHC||225||11276233|
|24426||mGluR1 alpha (Metabotropic Glutamate Receptor) Antibody||Neves, Guilherme, et al. "The LIM homeodomain protein Lhx6 regulates maturation of interneurons and network excitability in the mammalian cortex." Cerebral Cortex 23.8 (2013): 1811-1823.||Mouse||IF||6||22710612|
|24426||mGluR1 alpha (Metabotropic Glutamate Receptor) Antibody||Crepaldi, Luca, et al. "Transcriptional activators and repressors for the neuron-specific expression of a metabotropic glutamate receptor." Journal of Biological Chemistry 282.24 (2007): 17877-17889.||Human, Mouse||IF||Transcription Factor||11||17430891|
|24426||mGluR1 alpha (Metabotropic Glutamate Receptor) Antibody||Notenboom, Robbert GE, et al. "Up-regulation of hippocampal metabotropic glutamate receptor 5 in temporal lobe epilepsy patients." Brain 129.1 (2006): 96-107.||Rat||59||16311265|
Products You May Like
Q & A Wall
This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.Download MSDS