LHRH (Luteinizing Hormone Releasing Hormone) / GnRH Antibody

Image is of LHRH (GnRH) terminals in rat median eminence, ryostat sections, ABC / DAB detection
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Price: $250.00
Product ID : 20075

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Description

The antibody produces a strong postive labeling of LHRH / GnRH at dilution of 1/2,000 - 1,4,000 using biotin-streptavidin/HRP in rat hypothalamus (median eminence). Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.

The above image is of LHRH (GnRH) terminals in rat median eminence, ryostat sections, ABC / DAB detection.

Staining is completely eliminated by pretreatment of the diluted antibody with 5 µg of LHRH per mL of diluted antiserum.

Photo Description: IHC image of staining for LNRH/GnRH in the rat median eminence. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:4000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. 

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Reacts With: Bat, Carp, Catfish, Chick, Chicken, Clarias Batrachus (Catfish), Fish, Frog, Frog (Green Treefrog), Gerbil, Guinea Pig, Guinea Pig, Hagfish, Hamster, Human, Lizard, Mole Rat, Monkey, Mouse, Mudpuppy (Necturus Maculosus), Octopus, Pig, Rat, Salamander, Snail, Snail (Bulla Gouldiana), Squid, Trout

Availability: In Stock

Alternate Names:  Gonadotropin-Releasing Hormone; Gonadorelin; Luliberin I; Gonadoliberin I; progonadoliberin-1; GRH, anti-LHRH

Gene Symbol / ID, Accession #:  GNRH1, 2796

 RRID:  AB_572248

Reviews

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04/19/2012 - 03:19:35 PM
Highly recommend -- very specific staining with no background

I use the LHRH Antibody for immunohistochemistry on mouse embryonic tissue. I use cryostat sections of PFA fixed mouse heads, included in OCT.

I cut 10-20 microns sections, dry them and the process them as following: Protocol: 1hr blocking (PBS/0.1% triton plus 10% NGS), incubate overnight with LHRH antibody diluted in PBS/0.1% triton, 1:400. Then washes and secondary antibody fluorescent. When used with secondary biotinylated antibody, I use LHRH antibody less diluted (1:4000).

My student is also using the LHRH antibody on paraffin sections, with the same results (and also in conjunction with Tyramide amplification kit).

Staining is very specific. Stronger on adult tissue because more GnRH expressed by the neurons producing it. There is no background. Fixation sensitive; long fixations are not recommended. I highly recommend this antibody.


Anna Cariboni, PhD
Department of Cell and Developmental Biology
University College London

Publications

PROD # ANTIBODY CITATION SPECIES REACTIVITY APPL METHOD RESEARCH AREA # CITED BY PUBMED ID
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody RATKA, ANNA, and JAMES W. SIMPKINS. "A modulatory role for luteinizing hormone-releasing hormone in nociceptive responses of female rats." Endocrinology 127.2 (1990): 667-673. Rat 36 2197079
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Wirsig, C. R., and C. M. Leonard. "Acetylcholinesterase and luteinizing hormone-releasing hormone distinguish separate populations of terminal nerve neurons." Neuroscience 19.3 (1986): 719-740. 60 3540722
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Wang, Yong, et al. "Age affects spontaneous activity and depolarizing afterpotentials in isolated gonadotropin-releasing hormone neurons." Endocrinology 149.10 (2008): 4938-4947. Mouse 9 18583421
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Oosthuizen, M. K., N. C. Bennett, and C. W. Coen. "An immunohistochemical study of the gonadotrophin-releasing hormone 1 system in solitary Cape mole-rats,Georychus capensis, and social Natal mole-rats,Cryptomys hottentotus natalensis." Neuroscience 157.1 (2008): 164-173. Mole Rat 4 18817850
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody J Vasc Res. 1992 Jan-Feb;29(1):8-12. Vascular, ganglia and cardiac catecholamine disposition in the spontaneously hypertensive rat and in the stroke-prone spontaneously hypertensive rat. Mano M1, Jeffreson S, Head RJ. 96 15548653
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Pollak, Martin R., et al. "Calcium receptor stimulates chemotaxis and secretion." Am J Physiol Endocrinol Metab 292 (2007): E523-E532. Mouse IHC 0 17003237
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Bandyopadhyay, Sanghamitra, et al. "Calcium?sensing receptor stimulates secretion of an interferon???induced monokine (CXCL10) and monocyte chemoattractant protein?3 in immortalized GnRH neurons." Journal of neuroscience research 85.4 (2007): 882-895. Mouse 17 17285630
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Ma, Pokay M. "Catecholaminergic systems in the zebrafish. I. Number, morphology, and histochemical characteristics of neurons in the locus coeruleus." Journal of Comparative Neurology 344.2 (1994): 242-255. Fish 53 8077459
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Kalamatianos, T., et al. "Characterization of Gonadotrophin?Releasing Hormone Precursor cDNA in the Old World Mole?Rat Cryptomys Hottentotus Pretoriae: High Degree of Identity with the New World Guinea Pig Sequence." Journal of neuroendocrinology 17.5 (2005): 265-268. Mole Rat IHC DNA 4 15869560
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Kasten, Thomas L., et al. "Characterization of two new preproGnRH mRNAs in the tree shrew: first direct evidence for mesencephalic GnRH gene expression in a placental mammal." General and comparative endocrinology 104.1 (1996): 7-19. 125 8921350
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Cantwell, Elizabeth L., and Vincent M. Cassone. "Chicken suprachiasmatic nuclei: II. Autoradiographic and immunohistochemical analysis." Journal of Comparative Neurology 499.3 (2006): 442-457. Chicken IHC 12 16998905
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Brain Behav Evol. 1998;52(3):139-47. Coexistence of FMRFAMIDE-like and LHRH-like immunoreactivity in the terminal nerve and forebrain of the big brown bat, Eptesicus fuscus. Oelschläger HA1, Helpert C, Northcutt RG. Bat 16 9693160
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Yang, Mei-Fang, Chung-Liang Chien, and Kuo-Shyan Lu. "Compound 48/80-induced degranulation of GnRH-like immunoreactive mast cells in the brain and mesentery of the gerbil." ZOOLOGICAL STUDIES-TAIPEI- 41.1 (2002): 99-110. Gerbil 8 not found
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Memi, Fani, et al. "CXC Chemokine Receptor 7 (CXCR7) Affects the Migration of GnRH Neurons by Regulating CXCL12 Availability." The Journal of Neuroscience 33.44 (2013): 17527-17537. Mouse IHC 2 24174685
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Zhao, Sheng, and Lance J. Kriegsfeld. "Daily changes in GT1 Mouse 16 19141986
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Hum Mol Genet. 2011 Jan 15;20(2):336-44. doi: 10.1093/hmg/ddq468. Epub 2010 Nov 8. Defective gonadotropin-releasing hormone neuron migration in mice lacking SEMA3A signalling through NRP1 and NRP2: implications for the aetiology of hypogonadotropic hypogonadism. Cariboni A1, Davidson K, Rakic S, Maggi R, Parnavelas JG, Ruhrberg C. Mouse IF 21059704
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Gamble, John A., et al. "Disruption of ephrin signaling associates with disordered axophilic migration of the gonadotropin-releasing hormone neurons." The Journal of neuroscience 25.12 (2005): 3142-3150. Mouse ICC 36 15788771
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Braun, Christopher B., Helmut Wicht, and R. Glenn Northcutt. "Distribution of gonadotropin?releasinghormone immunoreactivity in the brain of the pacific hagfish, Eptatretus stouti (craniata: Myxinoidea)." Journal of Comparative Neurology 353.3 (1995): 464-476. Hagfish 28 7751443
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Fernandez-Galaz, Maria Carmen, et al. "Diurnal oscillation in glial fibrillary acidic protein in a perisuprachiasmatic area and its relationship to the luteinizing hormone surge in the female rat." Neuroendocrinology 70.5 (1999): 368-376. Rat IHC 22 10567863
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Ratka, Anna, and James W. Simpkins. "Effects of estradiol and progesterone on the sensitivity to pain and on morphine-induced antinociception in female rats." Hormones and behavior 25.2 (1991): 217-228. Rat 84 2066081
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Scott, Heather C., Willis K. Paull, and P. Kevin Rudeen. "Effects of in utero ethanol exposure on the development of LHRH neurons in the mouse." Developmental brain research 66.1 (1992): 119-125. Mouse 10 1600625
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Kuehl-Kovarik, M. Cathleen, et al. "Episodic bursting activity and response to excitatory amino acids in acutely dissociated gonadotropin-releasing hormone neurons genetically targeted with green fluorescent protein." The Journal of neuroscience 22.6 (2002): 2313-2322. Mouse 99 11896170
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Neurones, Releasing Hormone-Expressing. "Evidence for Oestrogen Receptor." Journal of Neuroendocrinology 11 (1999): 331-335. 0 not found
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Maggi, Mario, et al. "Expression and Biological Effects of Endothelin-1 in Human Gonadotropin-Releasing Hormone-Secreting Neurons 1." Journal of Clinical Endocrinology & Metabolism 85.4 (2000): 1658-1665. Human 17 10770212
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Horvath, Tamas L., Vito Cela, and Eline M. van der Beek. "Gender-specific apposition between vasoactive intestinal peptide-containing axons and gonadotrophin-releasing hormone-producing neurons in the rat." Brain research 795.1 (1998): 277-281. Rat 37 9622650
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Grober, Matthew S., et al. "GnRH cell size and number in a teleost fish with two male reproductive morphs: sexual maturation, final sexual status and body size allometry." Brain, behavior and evolution 43.2 (1994): 61-78. Fish 65 8143144
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Gaikwad, Archana, K. C. Biju, and Nishikant Subhedar. "GnRH-LH secreting cells axis in the pituitary of the teleostClarias batrachus responds to neuropeptide Y treatment: an immunocytochemical study." General and comparative endocrinology 131.2 (2003): 126-133. 20 12679089
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Grober, Matthew S., Ivor Jackson, and Andrew H. Bass. "Gonadal steroids affect LHRH preoptic cell number in a sex/role changing fish." Journal of neurobiology 22.7 (1991): 734-741. Fish 84 1765781
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Endocrinology. 2004 Aug;145(8):3608-12. Epub 2004 May 20. Gonadotropin releasing hormone-1 expression in incisors of mice. Tiong JD1, Pakiam JG, Wray S. Mouse IHC 15155575
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Wirsig-Wiechmann, Celeste R., et al. "Gonadotropin-Releasing Hormone Modulates Vomeronasal Neuron Response to Male Salamander Pheromone." Journal of Experimental Neuroscience 6 (2012). Salamander 0 not found
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody White, Stephanie A., and Russell D. Fernald. "Gonadotropin-releasing hormone-containing neurons change size with reproductive state in female Haplochromis burtoni." the Journal of Neuroscience 13.2 (1993): 434-441. Fish 54 8426222
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Grove-Strawser, Danielle, et al. "Guinea pig GnRH: localization and physiological activity reveal that it, not mammalian GnRH, is the major neuroendocrine form in guinea pigs." Endocrinology 143.5 (2002): 1602-1612. Guinea Pig 19 11956141
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Fasano, S., et al. "Hypothalamus-hypophysis and testicular GnRH control of gonadal activity in the frog,Rana esculenta: Seasonal GnRH profiles and annual variations ofin vitro androgen output by pituitary-stimulated testes." General and comparative endocrinology 70.1 (1988): 31-40. Frog 30 2836262
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Riddle, David R., and Bruce Oakley. "Immunocytochemical identification of primary olfactory afferents in rainbow trout." Journal of Comparative Neurology 324.4 (1992): 575-589. Trout IHC 57 1430339
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Ebersole, Ted J., and Sunny K. Boyd. "Immunocytochemical localization of gonadotropin-releasing hormones in the brain of a viviparous caecilian amphibian, Typhlonectes natans (Amphibia: Gymnophiona)." Brain, behavior and evolution 55.1 (2000): 14-25. 9 not found
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Subhedar, Nishikant, and N. S. Krishna. "Immunocytochemical localization of LH-RH in the brain and pituitary of the catfish,< i> Clarias batrachus(Linn.)." General and comparative endocrinology 72.3 (1988): 431-442. Catfish 52 3071489
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody J Comp Neurol. 1992 Jan 15;315(3):344-63. Immunocytochemical localization of luteinizing hormone-releasing hormone (LHRH) in the nervus terminalis and brain of the big brown bat, Eptesicus fuscus.Oelschläger HA1, Northcutt RG. Bat 22 1740548
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Masucci, M., et al. "Immunohistochemical demonstration of the presence and localization of diverse molecular forms of gonadotropin-releasing hormone in the lizard (Podarcis s. sicula) brain." General and comparative endocrinology 86.1 (1992): 81-89. Lizard 12 1505732
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Lamperti, Albert A., and Gary E. Pickard. "Immunohistochemical localization of luteinizing hormone?releasing hormone (LHRH) in the hypothalamus of adult female hamsters treated neonatally with monosodium glutamate or hypertonic saline." The Anatomical Record 209.1 (1984): 131-141. Hamster IHC 6 6375461
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Mackie, G. O. "Immunostaining of peripheral nerves and other tissues in whole mount preparations from hatchling cephalopods." Tissue and Cell 40.1 (2008): 21-29. Octopus, Squid IHC 13 17953977
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Jeong, K. H., et al. "Improvement in reproductive parameters in hypogonadal female mice by regulated gene replacement therapy in the central nervous system." Gene therapy 14.14 (2007): 1092-1101. Mouse 4 17476303
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Jain, Mukul R., and Nishikant K. Subhedar. "Increase in number of LHRH neurones in septal-preoptic area of rats following chronic amitriptyline treatment: implication in antidepressant effect." Brain research 604.1 (1993): 7-15. Rat 16 8457865
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody J Neurosci. 1998 Jan 1;18(1):559-72. Leptin receptor immunoreactivity in chemically defined target neurons of the hypothalamus. Hâkansson ML1, Brown H, Ghilardi N, Skoda RC, Meister B. Rat IF 644 9412531
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Magni, Paolo, et al. "Leukemia inhibitory factor induces the chemomigration of immortalized gonadotropin-releasing hormone neurons through the independent activation of the Janus kinase/signal transducer and activator of transcription 3, mitogen-activated protein kinase/extracellularly regulated kinase 1/2, and phosphatidylinositol 3-kinase/Akt signaling pathways." Molecular endocrinology 21.5 (2007): 1163-1174. Mouse IHC 19 17299136
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Roberts, Michael H., and Robert Y. Moore. "Localization of neuropeptides in efferent terminals of the eye in the marine snail, Bulla gouldiana." Cell and tissue research 248.1 (1987): 67-73. Snail 24 3552244
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Rossi, G. L., and G. Bestetti. "Morphological changes in the hypothalamic-hypophyseal-gonadal axis of male rats after twelve months of streptozotocin-induced diabetes." Diabetologia 21.5 (1981): 476-481. Rat diabete 61 7028562
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Eisthen, Heather L., et al. "Neuromodulatory effects of gonadotropin releasing hormone on olfactory receptor neurons." The Journal of Neuroscience 20.11 (2000): 3947-3955. Mudpuppy (Necturus Maculosus) IHC 126 10818129
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Sithigorngul, Paisarn, Antony OW Stretton, and Cynthia Cowden. "Neuropeptide diversity in Ascaris: an immunocytochemical study." Journal of Comparative Neurology 294.3 (1990): 362-376. 68 2341616
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Attademo, Andres M., et al. "Neuropeptide glutamic acid-isoleucine may induce luteinizing hormone secretion via multiple pathways." Neuroendocrinology 83.5-6 (2006): 313-324. IHC 12 17016031
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody J Neurosci. 2007 Feb 28;27(9):2387-95. Neuropilins and their ligands are important in the migration of gonadotropin-releasing hormone neurons. Cariboni A1, Hickok J, Rakic S, Andrews W, Maggi R, Tischkau S, Parnavelas JG. Mouse IF 17329436
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Sakharkar, Amul J., et al. "Neurosecretory neurons of the nucleus preopticus (NPO) express salmon GnRH mRNA and show reproduction phase-related variation in the female Indian major carp,Cirrhinus cirrhosus." Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology 151.2 (2008): 247-252. Carp IHC 2 18664387
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Kallo, I., et al. "Oestrogen Receptor-Immunoreactivity in Gonadotropin Releasing Hormone-Expressing Neurones: Regulation by Oestrogen." Journal of neuroendocrinology 13.9 (2001): 741-748. Mouse IHC 117 11578523
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Muske, Linda E., and Frank L. Moore. "Ontogeny of immunoreactive gonadotropin-releasing hormone neuronal systems in amphibians." Brain research 534.1 (1990): 177-187. 64 2073580
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Wright, D. E., and L. S. Demski. "Organization of GnRH and FMRF-amide systems in two primitive bony fishes (order polypteriformes)." Brain, behavior and evolution 47.5 (1996): 267-278. Fish IHC 33 8724648
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Santacana, M., A. G. De la Vega, and M. Heredia. "Peripherin fibers in the main olfactory bulb are different from olfactory fibers and from LHRH fibers: an immunocytochemical and DiI study." Brain research 734.1 (1996): 337-340. Rat 3 8896844
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody May, V., et al. "Persistence of immunoreactive TRH and GnRH in long-term primary anterior pituitary cultures." Peptides 8.3 (1987): 543-558. 43 2443903
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Jew, J. Y., et al. "Preoptic LH-RH and somatostatin in the rat median eminence." Neuroendocrinology 38.3 (1984): 169-175. Rat 23 6144060
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Physiol Behav. 1993 Sep;54(3):589-98. Reduction of motor impairment by adrenal medulla transplants in aged rats. García-Hernández F1, Pacheco-Cano MT, Drucker-Colín R. Rat 14 8415955
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Reelin provides an inhibitory signal in the migration of gonadotropin-releasing hormone neurons. Cariboni A1, Rakic S, Liapi A, Maggi R, Goffinet A, Parnavelas JG. Rat IF 16207762
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Du Toit, Lydia, et al. "Relations between social status and the gonadotrophin?releasing hormone system in females of two cooperatively breeding species of African mole?rats, Cryptomys hottentotus hottentotus and Cryptomys hottentotus pretoriae: Neuroanatomical and neuroendocrinological studies." Journal of Comparative Neurology 494.2 (2006): 303-313. Mole Rat IHC 5 16320240
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Biju, K. C., et al. "Reproduction phase-related expression of GnRH-like immunoreactivity in the olfactory receptor neurons, their projections to the olfactory bulb and in the nervus terminalis in the female Indian major carpCirrhinus mrigala(Ham.)." General and comparative endocrinology 133.3 (2003): 358-367. Carp (Fish) 25 12957480
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Sakharkar, Amul, Praful Singru, and Nishikant Subhedar. "Reproduction phase-related variations in the GnRH immunoreactive fibers in the pineal of the Indian major carp Cirrhinus mrigala (Ham.)." Fish physiology and biochemistry 31.2-3 (2005): 163-166. Carp ICC 4 20035452
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Gaikwad, A., et al. "Role of neuropeptide Y in the regulation of gonadotropin releasing hormone system in the forebrain ofClarias batrachus(Linn.): Immunocytochemistry and high performance liquid chromatography-electrospray ionization-mass spectrometric analysis." Neuroscience 133.1 (2005): 267-279. Clarias Batrachus (Catfish) 21 15893649
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Barni, Tullio, et al. "Sex Steroids and Odorants Modulate Gonadotropin-Releasing Hormone Secretion in Primary Cultures of Human Olfactory Cells 1." Journal of Clinical Endocrinology & Metabolism 84.11 (1999): 4266-4273. Human 40 10566683
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Palkovits, Miklos, et al. "Simultaneous characterization of pre-and postsynaptic neuron contact sites in brain." Proceedings of the National Academy of Sciences 79.8 (1982): 2705-2708. Rat ICC 9 6953424
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Cariboni, Anna, et al. "Slit2 and Robo3 modulate the migration of GnRH-secreting neurons." Development 139.18 (2012): 3326-3331. Mouse IF 2 22912413
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody White, Stephanie A., Tuan Nguyen, and Russell D. Fernald. "Social regulation of gonadotropin-releasing hormone." Journal of Experimental Biology 205.17 (2002): 2567-2581. Fish IHC 112 12151363
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Burmeister, Sabrina S., and Walter Wilczynski. "Social signals regulate gonadotropin-releasing hormone neurons in the green treefrog." Brain, behavior and evolution 65.1 (2004): 26-32. Frong (Green Treefrog) ICC 31 15489562
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Illig, Amanda M., et al. "Sodium valproate alters GnRH Mouse 11 11102573
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Patten, Bernard M., and Steve Croft. "Spinal cord substance P in amyotrophic lateral sclerosis." Research progress in motor neuron disease. Pitman, Bath (1984): 283-289. IHC 7 not found
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Fox, Helen E., et al. "Stress and dominance in a social fish." The Journal of Neuroscience 17.16 (1997): 6463-6469. Fish IHC 193 9236253
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Hahn, J. D., T. Kalamatianos, and C. W. Coen. "Studies on the Neuroanatomical Basis for Stress?Induced Oestrogen?Potentiated Suppression of Reproductive Function: Evidence Against Direct Corticotropin?Releasing Hormone Projections to the Vicinity of Luteinizing Hormone?Releasing Hormone Cell Bodies in Female Rats." Journal of neuroendocrinology 15.8 (2003): 732-742. Rat ICC 31 12834433
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Rossi, Giovanni L., and Gilberto Bestetti. "Technical aspects in the study of pathologic lesions in the hypothalamus of the rat." Endocrine System. Springer Berlin Heidelberg, 1983. 311-316. Rat 22 not found
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Roberts, Michael H., Joan C. Speh, and Robert Y. Moore. "The central nervous system of< i> Bulla gouldiana: Peptide localization." Peptides 9.6 (1988): 1323-1334. Snail (Bulla Gouldiana) IHC 22 3073358
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Gill, John C., et al. "The gonadotropin-releasing hormone (GnRH) neuronal population is normal in size and distribution in GnRH-deficient and GnRH receptor-mutant hypogonadal mice." Endocrinology 149.9 (2008): 4596-4604. Mouse IF 19 18499748
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Bushnik, Tamara L., and Russell D. Fernald. "The Population of GnRH-Containing Neurons Showing Socially Mediated Size Changes Project to the Pituitary in a Teleost, Hapiochromis burtoni." Brain, behavior and evolution 46.6 (1995): 371-377. Fish ICC 31 8719758
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Pearson, Paul L., Lloyd L. Anderson, and Carol D. Jacobson. "The prepubertal ontogeny of luteinizing hormone releasing hormone-like immunoreactivity in the diencephalon and telencephalon of the male Meishan pig brain." Developmental brain research 95.2 (1996): 157-168. Pig 1 8874890
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Liu, Shaojun, and Junyu Gao. "The relationship of galanin immunoreactive nerve fibers to glandular cells in the anterior pituitary in the monkey." Brain research 781.1 (1998): 114-120. Monkey IHC 9 9507082
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Murakami, Shizuko, and Yasumasa Arai. "Transient expression of somatostatin immunnoreactivity in the olfactory-forebrain region in the chick embryo." Developmental brain research 82.1 (1994): 277-285. Chick ICC 39 7842515
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody McLeod, Marcus C., et al. "Transplantation of GABAergic Cells Derived From Bioreactor-Expanded Human Neural Precursor Cells Restores Motor and Cognitive Behavioral Deficits in a Rodent Model of Huntington Rat 2 23127784
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Jastrow, Holger, Hynek Burda, and Helmut HA Oelschl 4 not found
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Development. 2011 Sep;138(17):3723-33. doi: 10.1242/dev.063362. VEGF signalling controls GnRH neuron survival via NRP1 independently of KDR and blood vessels. Cariboni A1, Davidson K, Dozio E, Memi F, Schwarz Q, Stossi F, Parnavelas JG, Ruhrberg C. Mouse IHC 21828096
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Rattazzi, Lorenza, et al. "Impaired sense of smell and altered olfactory system in RAG-1−∕− immunodeficient mice." Frontiers in neuroscience 9 (2015). Mouse 0 26441494
20075 GnRh / LHRH (Gonadotropin/Luteinizing Releasing Hormone) Antibody Tillo, Miguel, et al. "VEGF189 binds NRP1 and is sufficient for VEGF/NRP1-dependent neuronal patterning in the developing brain." Development 142.2 (2015): 314-319. Mouse 3 not defined

Q & A Wall

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I am interested in using LHRH antibody (Cat# 20075) for fixed cells ELISA assay. Do you recommend biotin-adivin/HRP system because it worked best for this antibody or you have not really tried anti rabbit IgG-HRP detection system?
Question By Dina

I see no reason why it wouldn't work. The use of anti-rabbit HRP is okay; they just need to work out an optimal concentration of LHRH aby and the secondary. I assume they will fix with formalin too...sometime cells are fixes in acetone which preserves some, but not all, antigens for immunohistochemistry so formalin is recommended.

Answer By Colleen on 2015-11-20 13:43:26

MSDS

This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.

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