Insulin Antibody

Insulin Antibody
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Price: $210.00
Product ID : 20056

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Description

The ImmunoStar Insulin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat pancreatic beta cells using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/3000-1/5000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique.

Immuostaining is completely abolished by soluble pre-adsorption with bovine insulin at 10 µg per mL of diluted antiserum.

Photo Description: IHC image of beta cells in the islets of Langerhans of the rat pancreas staining for insulin (above) and counterstained with hemotoxylin (below). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for frozen sectioning. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

Insulin pancreas #20056

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Reacts With: Bubalus Bubalis (Buffalo), Cattle, Chick, Cow, Heifer (Cow), Horse, Human, Lizard, Macaque, Manducta Sexta (Moth), Monkey, Mouse, Pig, Rat, Sheep

Availability: In Stock

Alternate Names:  anti-Insulin

Gene Symbol / ID, Accession #:  INS, 397415, 280829

RRID:  AB_572246


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MSDS

This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.

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