Insulin Antibody

Insulin Antibody
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Price: $210.00
Product ID : 20056

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Description

The ImmunoStar Insulin antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat pancreatic beta cells using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/3000-1/5000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique.

Immuostaining is completely abolished by soluble pre-adsorption with bovine insulin at 10 µg per mL of diluted antiserum.

Photo Description: IHC image of beta cells in the islets of Langerhans of the rat pancreas staining for insulin (above) and counterstained with hemotoxylin (below). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for frozen sectioning. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

Insulin pancreas #20056

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Reacts With: Bubalus Bubalis (Buffalo), Cattle, Chick, Cow, Heifer (Cow), Horse, Human, Lizard, Macaque, Manducta Sexta (Moth), Monkey, Mouse, Pig, Rat, Sheep

Availability: In Stock

Alternate Names:  anti-Insulin

Gene Symbol / ID, Accession #:  INS, 397415, 280829

RRID:  AB_572246


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Publications

PROD # ANTIBODY CITATION SPECIES REACTIVITY APPL METHOD RESEARCH AREA # CITED BY PUBMED ID
20056 Insulin Antibody Ravassard, Philippe, et al. "A new strategy to generate functional insulin-producing cell lines by somatic gene transfer into pancreatic progenitors." PloS one 4.3 (2009): e4731. Rat, Mouse IF 7 19266046
20056 Insulin Antibody Du, Yi-Chieh Nancy, David S. Klimstra, and Harold Varmus. "Activation of PyMT in Cells Induces Irreversible Hyperplasia, but Oncogene-Dependent Acinar Cell Carcinomas When Activated in Pancreatic Progenitors." PloS one 4.9 (2009): e6932. Mouse IHC 6 19812721
20056 Insulin Antibody Page, Benedict J., et al. "An immunocytochemical profile of the endocrine pancreas using an occlusive duct ligation model." Jop 1.4 (2000): 191-203. Rat ICC 14 11856861
20056 Insulin Antibody Jung, Hyo Won, et al. "Anti-diabetic effect of Wen-pi-tang-Hab-Wu-ling-san extract in streptozotocin-induced diabetic rats." Indian journal of pharmacology 44.1 (2012): 97. Rat IHC 4 22345879
20056 Insulin Antibody PLoS Biol. 2007 Oct 16;5(10):e276. Assessing tumor progression factors by somatic gene transfer into a mouse model: Bcl-xL promotes islet tumor cell invasion. Du YC1, Lewis BC, Hanahan D, Varmus H. Mouse IHC Tumor Markers 17941720
20056 Insulin Antibody Pang, Kevin, Chengeto Mukonoweshuro, and Gordon G. Wong. "Beta cells arise from glucose transporter type 2 (Glut2)-expressing epithelial cells of the developing rat pancreas." Proceedings of the National Academy of Sciences 91.20 (1994): 9559-9563. Rat IHC 133 7937805
20056 Insulin Antibody Hori, Masatsugu, et al. "Cholesterol feeding exacerbates myocardial injury in." Am. J. Physiol. Heart Circ. Physiol 278 (2000): H256-H262. Rat 0 10644606
20056 Insulin Antibody Mitra, Sudha Warrier, et al. "Colocalization of Somatostatin Receptor sst5 and Insulin in Rat Pancreatic ?-Cells 1." Endocrinology 140.8 (1999): 3790-3796. Rat IHC 81 10433240
20056 Insulin Antibody Naya, Francisco J., et al. "Diabetes, defective pancreatic morphogenesis, and abnormal enteroendocrine differentiation in BETA2/neuroD-deficient mice." Genes & Development 11.18 (1997): 2323-2334. Mouse IHC diabete 825 9308961
20056 Insulin Antibody Lab Invest. 2005 Jan;85(1):45-64. Disruption of IFT results in both exocrine and endocrine abnormalities in the pancreas of Tg737(orpk) mutant mice. Zhang Q1, Davenport JR, Croyle MJ, Haycraft CJ, Yoder BK. Mouse IF 15580285
20056 Insulin Antibody Usdin, Ted B., et al. "Distribution of the Parathyroid Hormone 2 Receptor in Rat: Immunolocalization Reveals Expression by Several Endocrine Cells 1." Endocrinology 140.7 (1999): 3363-3371. Rat IHC parathyroid 55 10385434
20056 Insulin Antibody RECANT, ULLIAN. "Effect of Dietaty Carbohydrates on Glucagon and Insulin Receptors in Genetically Obese Female Zucker Rats." History of Nutrition: History and Current Status of Human Energy Metabolism: Annual Meeting: Selected Papers.. The Institute, 1991. Rat 0 3039087
20056 Insulin Antibody Yasuda, Masahiko, et al. "Effects of growth factors on development of fetal islet B-cells in vitro." Journal of Veterinary Medical Science 69.8 (2007): 807. Rat IHC Growth Factors 12 17827886
20056 Insulin Antibody Castaing, M., et al. "Efficient restricted gene expression in beta cells by lentivirus-mediated gene transfer into pancreatic stem/progenitor cells." Diabetologia 48.4 (2005): 709-719. IHC 21 15759109
20056 Insulin Antibody Lacroix, M?C., et al. "Expression of insulin system in the olfactory epithelium: first approaches to its role and regulation." Journal of neuroendocrinology 20.10 (2008): 1176-1190. Rat 23 18752648
20056 Insulin Antibody BALTAZAR, EMMANUEL T., et al. "Galanin-like immunoreactive endocrine cells in bovine pancreas." Journal of anatomy 196.2 (2000): 285-291. Cattle IF 14 10739025
20056 Insulin Antibody Czernichow, Paul, Rapha, "Human pancreatic beta cell lines for diagnostic of diabetes" diabete 0 not found
20056 Insulin Antibody Oomori, Yukio, et al. "Immunocytochemical study of tyrosine hydroxylase and dopamine ?-hydroxylase immunoreactivities in the rat pancreas." Histochemistry 101.5 (1994): 313-323. Rat IHC 25 7523336
20056 Insulin Antibody Vittoria, Alfredo, et al. "Immunocytochemistry of paraneurons in the female urethra of the horse, cattle, sheep, and pig." The Anatomical Record 233.1 (1992): 18-24. Pig, Horse, Cow, Sheep IHC 11 1351370
20056 Insulin Antibody Endocr Dev. 2007;12:46-54. In vivo and in vitro techniques to study pancreas development and islet cell function. Duvillié B1, Heinis M, Stetsyuk V. 2 17923768
20056 Insulin Antibody Tay, S. S. W., and W. C. Wong. "Insulin-like immunoreactivity in the monkey spinal cord." Cells Tissues Organs 144.3 (1992): 196-201. Monkey 1 1529673
20056 Insulin Antibody Lipes, Myra A., et al. "Insulin-secreting non-islet cells are resistant to autoimmune destruction." Proceedings of the National Academy of Sciences 93.16 (1996): 8595-8600. Mouse IF 77 8710916
20056 Insulin Antibody Dheen, S. T., K. Rajkumar, and L. J. Murphy. "Islet cell proliferation and apoptosis in insulin-like growth factor binding protein-1 in transgenic mice." Journal of endocrinology 155.3 (1997): 551-558. Mouse Apoptosis 16 9488000
20056 Insulin Antibody Diabetes. 2003 Aug;52(8):2035-42. Label-retaining cells in the rat pancreas: location and differentiation potential in vitro. Duvillié B1, Attali M, Aiello V, Quemeneur E, Scharfmann R. Rat IHC 71 12882920
20056 Insulin Antibody KOMATSU, SUGURU, et al. "Maternal adrenocortical hormones maintain the early development of pancreatic B cells in the fetal rat." Journal of anatomy 193.4 (1998): 551-557. Rat IHC 7 10029188
20056 Insulin Antibody Elofsson, Rolf. "Monoaminergic and peptidergic neurons in the nervous system of Hutchinsoniella macracantha (Cephalocarida)." Journal of Crustacean Biology (1992): 531-536. IHC 25 not found
20056 Insulin Antibody Clewes, Christine, and Adrian Mander. "N05076: Report of the analysis of 25-hydroxy vitamin D to compare four different methods." (2007). 0 not found
20056 Insulin Antibody Danilova, Olga V., et al. "Neurogenin 3-specific dipeptidyl peptidase-2 deficiency causes impaired glucose tolerance, insulin resistance, and visceral obesity." Endocrinology 150.12 (2009): 5240-5248. Mouse IF obesity 10 19819973
20056 Insulin Antibody Sithigorngul, Paisarn, Antony OW Stretton, and Cynthia Cowden. "Neuropeptide diversity in Ascaris: an immunocytochemical study." Journal of Comparative Neurology 294.3 (1990): 362-376. 68 2341616
20056 Insulin Antibody Smith, S. B., et al. "Nitrogen and lipid metabolism in heifers fed at increasing levels of intake." Journal of animal science 70.1 (1992): 152-160. Heifer (Cow) 31 1582903
20056 Insulin Antibody Kim, Seung K., Matthias Hebrok, and Douglas A. Melton. "Notochord to endoderm signaling is required for pancreas development." Development 124.21 (1997): 4243-4252. Chick 369 9334273
20056 Insulin Antibody Lucini, C., et al. "Ontogeny, postnatal development and ageing of endocrine pancreas in Bubalus bubalis." Journal of anatomy 192.3 (1998): 417-424. Bubalus Bubalis (Buffalo) IHC 13 9688507
20056 Insulin Antibody Ku, Sae Kwang, and Jae Hyun Lee. "Original Articles: Immunohistochemical Study of the Pancreatic Endocrine Cells in the BALB/c mice: An Unique Distributional Pattern of Glucagon." Journal of veterinary science 3.3 (2002): 167-173. Mouse 8 12514327
20056 Insulin Antibody Nicol, Lindsey E., et al. "Pancreatic inflammation and increased islet macrophages in insulin-resistant juvenile primates." Journal of Endocrinology 217.2 (2013): 207-213. Monkey (Macaque) 4 23420316
20056 Insulin Antibody Rosenbaum, Tamara, et al. "Pancreatic cells synthesize and secrete nerve growth factor." Proceedings of the National Academy of Sciences 95.13 (1998): 7784-7788. Rat ICC 81 9636228
20056 Insulin Antibody Homberg, U., N. T. Davis, and J. G. Hildebrand. "Peptide?immunocytochemistry of neurosecretory cells in the brain and retrocerebral complex of the sphinx moth Manduca sexta." Journal of comparative neurology 303.1 (1991): 35-52. Manducta Sexta (Moth) 91 1706364
20056 Insulin Antibody Yamamoto, Masako, et al. "Recovery in the fetal pancreatic islet following fetal administration of streptozotocin in the rat in vivo and in vitro." The Anatomical Record Part A: Discoveries in Molecular, Cellular, and Evolutionary Biology 281.2 (2004): 1319-1325. Rat IHC 5 15515161
20056 Insulin Antibody Dunn, P. J., et al. "Recurrent insulinoma syndrome with metastatic glucagonoma." Journal of clinical pathology 36.9 (1983): 1076-1080. Human 5 6136529
20056 Insulin Antibody Elghazi, Lynda, et al. "Role for FGFR2IIIb-mediated signals in controlling pancreatic endocrine progenitor cell proliferation." Proceedings of the National Academy of Sciences 99.6 (2002): 3884-3889. Rat IHC 107 11891329
20056 Insulin Antibody Heart, Emma, et al. "Role for malic enzyme, pyruvate carboxylation, and mitochondrial malate import in glucose-stimulated insulin secretion." American Journal of Physiology-Endocrinology And Metabolism 296.6 (2009): E1354-E1362. Mouse ICC 19 19293334
20056 Insulin Antibody COHN, DAVID V., et al. "Selective Localization of the Parathyroid Secretory Protein-I/Adrenal Medulla Chromogranin A Protein Family in a Wide Variety of Endocrine Cells of the Rat*." Endocrinology 114.6 (1984): 1963-1974. Rat IF parathyroid 204 6233131
20056 Insulin Antibody Taniyama, H., et al. "Spontaneous diabetes mellitus associated with persistent bovine viral diarrhea (BVD) virus infection in young cattle." Veterinary Pathology Online 32.3 (1995): 221-229. Cattle IHC diabete 17 7604488
20056 Insulin Antibody Eur J Pharmacol. 2009 Mar 1;605(1-3):164-9. doi: 10.1016/j.ejphar.2009.01.001. Epub 2009 Jan 10.Telmisartan, an angiotensin II type 1 receptor blocker, prevents the development of diabetes in male Spontaneously Diabetic Torii rats. Hasegawa G1, Fukui M, Hosoda H, Asano M, Harusato I, Tanaka M, Shiraishi E, Senmaru T, Sakabe K, Yamasaki M, Kitawaki J, Fujinami A, Ohta M, Obayashi H, Nakamura N. Rat IF diabete 19171132
20056 Insulin Antibody Pin, Christopher L., et al. "The bHLH transcription factor Mist1 is required to maintain exocrine pancreas cell organization and acinar cell identity." The Journal of cell biology 155.4 (2001): 519-530. Mouse Transcription Factor 142 11696558
20056 Insulin Antibody Genes Dev. 2003 Dec 15;17(24):3127-38. Epub 2003 Dec 17.The c-myc and PyMT oncogenes induce different tumor types in a somatic mouse model for pancreatic cancer. Lewis BC1, Klimstra DS, Varmus HE. Mouse IF cancer 14681205
20056 Insulin Antibody Ku, S. K., and H. S. Lee. "The distribution and frequency of endocrine cells in the splenic lobe of grass lizard (Takydromus wolteri): An immunohistochemical study." European journal of histochemistry 48.4 (2009): 429-436. Lizard 5 15718210
20056 Insulin Antibody Stellrecht, Christine MM, et al. "Tissue-specific and developmental regulation of the rat insulin II gene enhancer, RIPE3, in transgenic mice." Journal of Biological Chemistry 272.6 (1997): 3567-3572. Mouse IHC 23 9013607
20056 Insulin Antibody Nicol, Lindsey E., et al. "Abnormal infant islet morphology precedes insulin resistance in PCOS-like monkeys." (2014): e106527. Monkey 4 25207967

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MSDS

This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.

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