Histamine Antibody

Histamine Antibody
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Price: $275.00
Product ID : 22939



Histamine is located in mast cells, endocrine cells of the gut, blood cells and in some cells of the peripheral and central nervous system. Histamine is a potent vasodilator when secreted by mast cells found in various tissues as a result of allergic hypersensitivity or inflammation. In the central nervous system, Histamine is putative neurotransmitter. In the brain, its highest content has been found in the hypothalamus and in certain areas of the mesencephalon. The Histamine antiserum has a sensitivity level capable of detecting the low level Histamine contents of the brain.

The Histamine antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat hypothalamus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/4000-1/6000 in PBS/0.3% Triton X-100 biotin/avidin-HRP Technique . All staining is blocked by preabsorption of the antiserum with Histamine conjugate. Cross reactivity experiments indicate no cross reactivity with L-histidine or L-histidine containing peptides such as LH-RH.

Photo Description: IHC image of neurons staining for histamine in the rat hypothalamus. The tissue was fixed with 4% carbodimide in 0.05 M phosphate buffered saline, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. The section was then mounted on slides with permount.

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Reacts With: Aplysia Californica (Sea Slug), Bee, Copepods (Crustacean), Crab, Drosophila (Fly), Drosophila Melanogaster (Fly), Flea, Fly, Fruit Fly, Locust, Marine, Monkey, Moth, Mouse, Pig, Pleurobranchaea Japonica (Sea Slug), Rat, Snail

Availability: In Stock

Alternate Names: anti-Histamine

RRID:  AB_572245


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Reviewed by adacks
01/21/2013 - 11:27:32 AM
Good antibody, mediocre penetration

I have used this antibody (#22939) to label the brains of ~20 different species of insects. Brains are embedded in agarose and sectioned on a vibrating microtome at 50-70um. Note that the 4 hour carbodiimide fixation step (see below) is ABSOLUTELY necessary for the labeling to work. Pre-adsorbing the antibody with KLH does not affect labeling (see Dacks et al 2010, J. Comp. Neurol.). This antibody does not have great penetration for using a vibrating microtome, but 50um sections with 1% TX-100 for incubation with the primary is sufficient.

Day 1:
_____Dissect brain in saline
_____fix brains in 4% 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide in phosphate buffered saline (PBS; pH 6.9) for 4hrs at 4°C
_____Post-fix overnight in 4% paraformaldehyde at 4°C

Day 2:
_____3X 15min PBS washes
_____Embed brains in agarose and section at 50µm
_____4X 15min in PBS with .5% Triton X-100 (PBST) room temperature (RT)
_____Block tissue for 1hr in 2% IgG free Bovine Serum Albumin (BSA)
_____Incubate sections in 2% BSA in PBST (1%TX-100) with 50 mM sodium azide (PBSAT) with 1:500 Histamine (Immunostar # 22939) for 24hrs at RT

Day 3:
_____4 X 15min PBST at RT
_____Block tissue for 1hr (2% BSA)
_____Spin down secondary in PBSAT with 2% BSA for 15min at 14,000RPM
_____Incubate in block (PBSAT) with 1:1000 Cy3 Gt Rb 2° overnight at RT

Day 4:
_____2x 30min wash in PBST
_____2x 30min wash in PBS
_____5 min in 60% glycerol
_____Mount in 80% glycerol

Only use 1% Triton X-100 for primary, otherwise always use .5%.

Andrew Dacks
Assistant Professor
West Virginia University


22939 Histamine Antibody PLoS Genet. 2008 Nov;4(11):e1000245. doi: 10.1371/journal.pgen.1000245. Epub 2008 Nov 7.A glial variant of the vesicular monoamine transporter is required to store histamine in the Drosophila visual system. Romero-Calderón R1, Uhlenbrock G, Borycz J, Simon AF, Grygoruk A, Yee SK, Shyer A, Ackerson LC, Maidment NT, Meinertzhagen IA, Hovemann BT, Krantz DE.3 Fly IF 18989452
22939 Histamine Antibody Bergman, Hanna, et al. "Characterization of Histaminergic H 3 Receptors in Intraocular Tuberomammillary Transplants Containing Histaminergic Neurons." Experimental neurology 136.1 (1995): 12-21. 2 7589330
22939 Histamine Antibody Kawazoe, Yoshinori, Satoshi Tanaka, and Motonari Uesugi. "Chemical genetic identification of the histamine H1 receptor as a stimulator of insulin-induced adipogenesis." Chemistry & biology 11.7 (2004): 907-913. Mouse 13 15271349
22939 Histamine Antibody Ervin, Wendi-Jo L. "Co-localization of Histamine and eGFP in the Central Nervous System from pHdc-5 0 not found
22939 Histamine Antibody Paulk, Angelique C., Andrew M. Dacks, and Wulfila Gronenberg. "Color processing in the medulla of the bumblebee (Apidae: Bombus impatiens)." Journal of Comparative Neurology 513.5 (2009): 441-456. 22 not found
22939 Histamine Antibody Orcine, Monica. "Comparative study of histaminergic neurons in two calanoid copepods." (2011). Copepods (Crustacean) 0 not found
22939 Histamine Antibody Wilson, James R., et al. "Comparison of cholinergic and histaminergic axons in the lateral geniculate complex of the macaque monkey." The Anatomical Record 255.3 (1999): 295-305. Monkey 16 10411396
22939 Histamine Antibody Microsc Res Tech. 2011 Oct;74(10):946-56. doi: 10.1002/jemt.20980. Epub 2011 Apr 11. Development of histamine-immunoreactivity in the Central nervous system of the two locust species Schistocerca gregaria and Locusta migratoria. Pätschke A1, Bicker G. Locust ICC 1 21484940
22939 Histamine Antibody Evodevo. 2012 Jan 24;3:3. doi: 10.1186/2041-9139-3-3. Development of the larval anterior neurogenic domains of Terebratalia transversa (Brachiopoda) provides insights into the diversification of larval apical organs and the spiralian nervous system. Santagata S1, Resh C, Hejnol A, Martindale MQ, Passamaneck YJ. Marine IF 22273002
22939 Histamine Antibody Ellender, Tommas J., et al. "Differential modulation of excitatory and inhibitory striatal synaptic transmission by histamine." The Journal of Neuroscience 31.43 (2011): 15340-15351. Mouse 22 22031880
22939 Histamine Antibody Borycz, J., et al. "Drosophila ABC transporter mutants white, brown and scarlet have altered contents and distribution of biogenic amines in the brain." Journal of Experimental Biology 211.21 (2008): 3454-3466. Drosophila Melanogaster (Fly) IF 43 18931318
22939 Histamine Antibody Wagner, Stefanie, et al. "Drosophila photoreceptors express cysteine peptidase tan." Journal of Comparative Neurology 500.4 (2007): 601-611. Fruit Fly IF 21 17154266
22939 Histamine Antibody Chen, Gang, et al. "Effect of a histamine H1 receptor antagonist on gastric endocrine cell proliferation induced by chronic acid suppression in rats." Journal of gastroenterology 35.10 (2000): 742-747. Rat IHC 8 11063217
22939 Histamine Antibody Omura, Nobuo, et al. "Effect of pirenzepine on gastric endocrine cell kinetics during lansoprazole administration." Journal of gastroenterology 33.5 (1998): 634-639. 3 9773926
22939 Histamine Antibody Santagata, Scott. "Evaluating neurophylogenetic patterns in the larval nervous systems of brachiopods and their evolutionary significance to other bilaterian phyla." Journal of morphology 272.10 (2011): 1153-1169. 2 21598293
22939 Histamine Antibody Braubach, Oliver R., and Roger P. Croll. "Evidence that histamine acts as a neurotransmitter in statocyst hair cells in the snail, Lymnaea stagnalis." J. Gravit. Physiol 11 (2004): 57-66. 3 not found
22939 Histamine Antibody Barth, Martin, et al. "Experience-dependent developmental plasticity in the optic lobe of Drosophila melanogaster." The Journal of neuroscience 17.4 (1997): 1493-1504. Drosophila Melanogaster (Fly) IHC 98 9006990
22939 Histamine Antibody Rioux, Kevin P., et al. "Hepatic mucosal mast cell hyperplasia in rats with secondary biliary cirrhosis." Hepatology 23.4 (1996): 888-895. Rat IHC 49 8666346
22939 Histamine Antibody Hong, Sung-Tae, et al. "Histamine and its receptors modulate temperature-preference behaviors in Drosophila." The Journal of neuroscience 26.27 (2006): 7245-7256. Drosophila (Fly) 52 16822982
22939 Histamine Antibody Jacobi, Henrik H., et al. "Histamine immunocytochemistry: a new method for detection of basophils in peripheral blood." Journal of immunological methods 237.1 (2000): 29-37. 4 10725449
22939 Histamine Antibody Buchner, Erich, et al. "Histamine is a major mechanosensory neurotransmitter candidate in Drosophila melanogaster." Cell and tissue research 273.1 (1993): 119-125. Drosophila Melanogaster (Fly) 65 8103421
22939 Histamine Antibody Oh, Yangkyun, et al. "Histamine-HisCl1 Receptor Axis Regulates Wake-Promoting Signals in Drosophila melanogaster." PloS one 8.7 (2013): e68269. Fly IHC 0 23844178
22939 Histamine Antibody Dacks, Andrew M., et al. "Histamine?immunoreactive local neurons in the antennal lobes of the hymenoptera." Journal of Comparative Neurology 518.15 (2010): 2917-2933. Bee ICC 14 20533353
22939 Histamine Antibody J Exp Biol. 2011 May 15;214(Pt 10):1773-82. doi: 10.1242/jeb.054486. Histaminergic signaling in the central nervous system of Daphnia and a role for it in the control of phototactic behavior. McCoole MD1, Baer KN, Christie AE. Flea IF 21525325
22939 Histamine Antibody Curtis, M., et al. "Hypothalamic tissue stimulates hippocampal pyramidal neuron survival during development: evidence from intraocular double transplants." Hippocampus 5.6 (1995): 584-594. 4 8646284
22939 Histamine Antibody Messinger, Daniel I., et al. "Identification and characterization of a tachykinin-containing neuroendocrine organ in the commissural ganglion of the crab Cancer productus." Journal of experimental biology 208.17 (2005): 3303-3319. Crab IF cancer 32 16109892
22939 Histamine Antibody Xu, Yanli, Leonard J. Cleary, and John H. Byrne. "Identification and characterization of pleural neurons that inhibit tail sensory neurons and motor neurons in Aplysia: correlation with FMRFamide immunoreactivity." The Journal of neuroscience 14.6 (1994): 3565-3577. Aplysia Californica (Sea Slug) IHC 27 8207472
22939 Histamine Antibody Iwano, Masaaki, and Ryohei Kanzaki. "Immunocytochemical identification of neuroactive substances in the antennal lobe of the male silkworm moth Bombyx mori." Zoological science 22.2 (2005): 199-211. Moth 22 15738640
22939 Histamine Antibody Ohning, Gordon V., et al. "Immunolocalization of gastrin-dependent histidine decarboxylase activity in rat gastric mucosa during feeding." American Journal of Physiology-Gastrointestinal and Liver Physiology 275.4 (1998): G660-G667. Rat 11 9756494
22939 Histamine Antibody Bergman, H., J. I. Nagy, and A-Ch Granholm. "Intracranial transplantation and survival of tuberomammillary histaminergic neurons." Neuroscience 64.1 (1995): 61-70. 9 7708215
22939 Histamine Antibody Granholm, Ann-Charlotte, et al. "Intraocular hypothalamic transplants containing histaminergic neurons: Innervation of host iris and hippocampal cografts." Experimental neurology 108.3 (1990): 189-197. 5 1693577
22939 Histamine Antibody Fang, Yang-Il, et al. "Marked increase in the histamine content of neointima after stent implantation of pig coronary artery and growth-promoting effects of histamine in cultured smooth muscle cells." Life sciences 77.3 (2005): 241-251. Pig IHC 8 15878353
22939 Histamine Antibody Silverman, Ann-Judith, et al. "Mast cells migrate from blood to brain." The Journal of Neuroscience 20.1 (2000): 401-408. Rat ICC 111 10627616
22939 Histamine Antibody Puri, Niti, and Paul A. Roche. "Mast cells possess distinct secretory granule subsets whose exocytosis is regulated by different SNARE isoforms." Proceedings of the National Academy of Sciences 105.7 (2008): 2580-2585. Mouse IHC 86 18250339
22939 Histamine Antibody Ohsuga, Kenji, Makoto Kurokawa, and Kiyoaki Kuwasawa. "Mosaic arrangement of SCPB-, FMRFamide-, and histamine-like immunoreactive sensory hair cells in the statocyst of the gastropod mollusc Pleurobranchaea japonica." Cell and tissue research 300.1 (2000): 165-172. Pleurobranchaea Japonica (Sea Slug) 14 10805085
22939 Histamine Antibody Wyeth, Russell C., and Roger P. Croll. "Peripheral sensory cells in the cephalic sensory organs of Lymnaea stagnalis." Journal of Comparative Neurology 519.10 (2011): 1894-1913. Snail IHC 3 21452209
22939 Histamine Antibody Blair, P. J., et al. "Relationship between enteric neurons and interstitial cells in the primate gastrointestinal tract." Neurogastroenterology & Motility 24.9 (2012): e437-e449. Monkey 5 not found
22939 Histamine Antibody Bischoff, Stephan C., et al. "Role of serotonin in intestinal inflammation: knockout of serotonin reuptake transporter exacerbates 2, 4, 6-trinitrobenzene sulfonic acid colitis in mice." American Journal of Physiology-Gastrointestinal and Liver Physiology 296.3 (2009): G685-G695. Mouse ulcerative colitis 48 19095763
22939 Histamine Antibody J Neurosci. 1998 Sep 15;18(18):7160-6. Selective histamine uptake rescues photo- and mechanoreceptor function of histidine decarboxylase-deficient Drosophila mutant. Melzig J1, Burg M, Gruhn M, Pak WL, Buchner E. Drosophila (Fly) IHC 9736639
22939 Histamine Antibody J Neurosci. 2002 Dec 15;22(24):10549-57. tan and ebony genes regulate a novel pathway for transmitter metabolism at fly photoreceptor terminals. Borycz J1, Borycz JA, Loubani M, Meinertzhagen IA Fly IF 12486147
22939 Histamine Antibody Brunjes, Peter C., Rachel B. Kay, and J. P. Arrivillaga. "The mouse olfactory peduncle." Journal of Comparative Neurology 519.14 (2011): 2870-2886. Mouse IF 5 23355812
22939 Histamine Antibody McCann, Conor J., et al. "Bone marrow derived Kit-positive cells colonize the gut but fail to restore pacemaker function in intestines lacking interstitial cells of Cajal." Journal of neurogastroenterology and motility 20.3 (2014): 326. Mouse WB, IF 1 24847840
22939 Histamine Antibody Noseda, Rodrigo, et al. "Neurochemical pathways that converge on thalamic trigeminovascular neurons: potential substrate for modulation of migraine by sleep, food intake, stress and anxiety." (2014): e103929. 8 25090640

Q & A Wall

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I am planning to do some single immunoreactions using the biotin streptavidin/HRP technique and I would like to know the right protocol I should follow. I read from the data sheet that the perfusion/fixation should be done using 4% carbodiimide in PBS. I am interested in doing electron microscopic analysis on this material. Is it possible to add glutaraldehyde(0.1%) to the 4% carbodiimide-PBS?
Question By Icnelia H.

No answer found for this question.

I need to know the cross-reactivity of this antibody.
Question By Katsura

The only cross-reactivity information we have is that which is included on the specification sheet: no cross-reactivity with L-histidine or L-histidine-containing peptides such as LH-RH. This testing was done in-house, by dot-blot.

Answer By Jodi on 2009-03-06 12:02:25


This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.

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