Glutamate Antibody

Glutamate Antibody
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Price: $440.00
Product ID : 22523

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Description

The monoclonal antibody produces strong labeling of glutamate at dilutions of 1/2,000 - 1/4,000 using biotin-streptavidin/HRP technique. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.

Glutamate tissue staining is completely eliminated by preincubation with glutamate conjugate at concentrations of 100 µg conjugate per mL of diluted antiserum. Aspartate and glutamine conjugates could not significantly inhibit tissue staining. The following amino acids were tested for cross reactivity using an enzyme-linked immunoassay method. Wells were coated with Glu-Glut-Btg at 1 µg per mL. Amino acids and conjugate were added at concentrations from 10 µg to 1 ng per mL. The Glutamate antibody was added to wells at 1 µg per mL. These amino acids were found to have cross reactivity at less than 1%: Beta-alanine, L-alanine, L-aspartic acid, L-glutamic acid, Glycine, Taurine and L-tyrosine.

Photo Description: IHC image of neurons staining for glutamate in the rat cortex (above) and in a low magnification image of the rat hippocampus (below). The tissue was fixed with 4% formaldehyde/0.3% glutaraldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:4000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

Glutamate hippocampus #22523

Host: Mouse

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Reacts With: Bathynomus Doederleini (Crustacean/Isopod), Cat, Cockroach, Crayfish, Fish, Hamster, Human, Leech, Monkey, Mouse, Panulirus Argus (Lobster), Rabbit, Rat, Squilla Oratoria (Shrimp)

Availability: In Stock

Alternate Names:
anti-Glutamate

RRID:  AB_572244

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Publications

PROD # ANTIBODY CITATION SPECIES REACTIVITY APPL METHOD RESEARCH AREA # CITED BY PUBMED ID
22523 Glutamate Antibody Brooks, P. A. "8-Hydroxy-2-(Di-N-Propylamino) Tetralin, which has high affinity for 5." 0 not found
22523 Glutamate Antibody S. Mccasland, Lyndon S. Hibbard, Robert W. Rhoades, Thomas A. Woolsey, James. "Activation of a wide-spread network of inhibitory neurons in barrel cortex." Somatosensory & motor research 14.2 (1997): 138-147. 17 9399415
22523 Glutamate Antibody Morales, F. R., et al. "Brainstem glycinergic neurons and their activation during active (rapid eye movement) sleep in the cat." Neuroscience 142.1 (2006): 37-47. Cat IHC 24 16891059
22523 Glutamate Antibody Chen, Quan, C. L. Veenman, and A. Reiner. "Cellular expression of ionotropic glutamate receptor subunits on specific striatal neuron types and its implication for striatal vulnerability in glutamate receptor-mediated excitotoxicity." Neuroscience 73.3 (1996): 715-731. 125 8809793
22523 Glutamate Antibody Uematsu, Kazumasa, et al. "Central mechanisms underlying fish swimming." Brain, behavior and evolution 69.2 (2007): 142-150. Fish ICC 6 17230022
22523 Glutamate Antibody Vizuete, M. L., et al. "Changes in neurotransmitters in superior colliculus after neonatal enucleation: biochemical and immunocytochemical studies." Neuroscience 56.1 (1993): 165-176. 6 7901805
22523 Glutamate Antibody Synapse. 2005 Dec 1;58(3):151-64. Characterization of medial septal glutamatergic neurons and their projection to the hippocampus.Colom LV1, Castaneda MT, Reyna T, Hernandez S, Garrido-Sanabria E. Rat IF 16108008
22523 Glutamate Antibody Maqbool, Azhar, Peter N. McWilliam, and Trevor FC Batten. "Co-localization ofc-Fos and neurotransmitter immunoreactivities in the cat brain stem after carotid sinus nerve stimulation." Journal of chemical neuroanatomy 13.3 (1997): 189-200. Cat 10 9315968
22523 Glutamate Antibody J Neurosci. 2000 May 1;20(9):3319-27. Cuneiform neurons activated during cholinergically induced active sleep in the cat.Pose I1, Sampogna S, Chase MH, Morales FR. Cat IHC 11 10777795
22523 Glutamate Antibody Redburn, Dianna A., et al. "Development of the glutamate system in rabbit retina." Neurochemical research 17.1 (1992): 61-66. Rabbit 41 1347162
22523 Glutamate Antibody Nagayama, Toshiki, et al. "Distribution of glutamatergic immunoreactive neurons in the terminal abdominal ganglion of the crayfish." Journal of Comparative Neurology 474.1 (2004): 123-135. Crayfish ICC 4 15156582
22523 Glutamate Antibody J Neurosci. 1998 Jun 15;18(12):4588-602. Dopamine neurons make glutamatergic synapses in vitro. Sulzer D1, Joyce MP, Lin L, Geldwert D, Haber SN, Hattori T, Rayport S. Monkey, Rat IF 9614234
22523 Glutamate Antibody Hu, Limin, John D. Fernstrom, and Paul C. Goldsmith. "Exogenous glutamate enhances glutamate receptor subunit expression during selective neuronal injury in the ventral arcuate nucleus of postnatal mice." Neuroendocrinology 68.2 (1998): 77-88. Mouse 23 9705574
22523 Glutamate Antibody Korada, Sailaja, et al. "Fibroblast growth factor 2 is necessary for the growth of glutamate projection neurons in the anterior neocortex." The Journal of neuroscience 22.3 (2002): 863-875. Mouse 59 11826116
22523 Glutamate Antibody Peterson, Daniel A., et al. "Fibroblast growth factor-2 protects entorhinal layer II glutamatergic neurons from axotomy-induced death." Journal of Neuroscience 16.3 (1996): 886-898. Rat IF 91 8558257
22523 Glutamate Antibody McCasland, James S., and Lyndon S. Hibbard. "GABAergic neurons in barrel cortex show strong, whisker-dependent metabolic activation during normal behavior." The Journal of neuroscience 17.14 (1997): 5509-5527. Hamster IHC metabolic 46 9204933
22523 Glutamate Antibody Kowall, Neil W., and M. Flint Beal. "Glutamate, glutaminase, and taurine immunoreactive neurons develop neurofibrillary tangles in Alzheimer's disease." Annals of neurology 29.2 (1991): 162-167. IHC alzheimer 67 1672808
22523 Glutamate Antibody Brodfuehrer, Peter D., and Avis H. Cohen. "Glutamate-like immunoreactivity in the leech central nervous system." Histochemistry 97.6 (1992): 511-516. Leech IHC 11 1358864
22523 Glutamate Antibody Lee, Hyun S., et al. "Glutamatergic afferent projections to the dorsal raphe nucleus of the rat." Brain research 963.1 (2003): 57-71. Rat 91 12560111
22523 Glutamate Antibody Daadi, Marcel M. "In vitro assays for neural stem cell differentiation." Neural Stem Cells: Methods and Protocols. Humana Press, 2002. 149-155. IHC Stem Cell 9 11951617
22523 Glutamate Antibody V Colom, Luis, et al. "Intrahippocampal Amyloid-beta (1-40) injections injure medial septal Neurons in rats." Current Alzheimer Research 8.8 (2011): 832-840. Rat 9 22044024
22523 Glutamate Antibody Fricker-Gates, Rosemary A., et al. "Late-stage immature neocortical neurons reconstruct interhemispheric connections and form synaptic contacts with increased efficiency in adult mouse cortex undergoing targeted neurodegeneration." The Journal of neuroscience 22.10 (2002): 4045-4056. Mouse 73 12019324
22523 Glutamate Antibody Tong, Qingchun, and Annette L. Kirchgessner. "Localization and function of metabotropic glutamate receptor 8 in the enteric nervous system." American Journal of Physiology-Gastrointestinal and Liver Physiology 285.5 (2003): G992-G1003. Rat ICC 30 12829438
22523 Glutamate Antibody Delgado, Jary Y., Eduardo Oyola, and Mark W. Miller. "Localization of GABA-and glutamate-like immunoreactivity in the cardiac ganglion of the lobster Panulirus argus." Journal of neurocytology 29.8 (2000): 605-619. Panulirus Argus (Lobster) 27 11283415
22523 Glutamate Antibody Colom LV1, Castañeda MT, Bañuelos C, Puras G, García-Hernández A, Hernandez S, Mounsey S, Benavidez J, Lehker C, Neurobiol Aging. 2010 Jan;31(1):46-57. doi: 10.1016/j.neurobiolaging.2008.05.006. Epub 2008 Jun 10."Medial septal beta-amyloid 1-40 injections alter septo-hippocampal anatomy and function" Rat IHC 28 18547680
22523 Glutamate Antibody Colom, L. V., et al. "Memantine protects cholinergic and glutamatergic septal neurons from A?< 1 Rat 2 23458672
22523 Glutamate Antibody Cassella, J. P., K. Andrews, and S. Y. Ali. "Microanalytical studies of bone mineral in osteogenesis imperfecta." Bone 13.1 (1992): 101-102. 1 not found
22523 Glutamate Antibody Cocanougher, Benjamin T. "Microvessel Regulation by Tyrosine Hydroxylase: Immunoreactive Interneurons in the Developing Rat Cortex and the Adult Human Cerebral Cortex." (2010). Rat, Human 0 not found
22523 Glutamate Antibody Morris, Judy L., et al. "Most peptide-containing sensory neurons lack proteins for exocytotic release and vesicular transport of glutamate." Journal of Comparative Neurology 483.1 (2005): 1-16. IF 44 15672399
22523 Glutamate Antibody Vadivelu, Sudhakar, et al. "Multi-germ layer lineage central nervous system repair: nerve and vascular cell generation by embryonic stem cells transplanted in the injured brain." Journal of neurosurgery 103.1 (2005): 124-135. Stem Cell 24 16121983
22523 Glutamate Antibody Park, Kook In, et al. "Neural stem cells may be uniquely suited for combined gene therapy and cell replacement: evidence from engraftment of neurotrophin-3-expressing stem cells in hypoxic Stem Cell 156 16714016
22523 Glutamate Antibody Goldsmith, Paul C. "Neuroglial responses to elevated glutamate in the medial basal hypothalamus of the infant mouse." The Journal of nutrition 130.4 (2000): 1032S-1038S. Mouse ICC 15 10736376
22523 Glutamate Antibody Yoko, F., and Kiyoaki Kuwasawa. "Neurohormonal and glutamatergic neuronal control of the cardioarterial valves in the isopod crustacean Bathynomus doederleini." Journal of experimental biology 206.3 (2003): 431-443. Bathynomus Doederleini (Crustacean/Isopod) ICC 5 12502764
22523 Glutamate Antibody Ando, Hiroshi, and Kiyoaki Kuwasawa. "Neuronal and neurohormonal control of the heart in the stomatopod crustacean, Squilla oratoria." Journal of experimental biology 207.26 (2004): 4663-4677. Squilla Oratoria (Shrimp) ICC 15 15579561
22523 Glutamate Antibody Schoenen, Jean, et al. "Neurotransmitter phenotype plasticity in cultured dissociated adult rat dorsal root ganglia: an immunocytochemical study." Journal of neuroscience research 22.4 (1989): 473-487. Rat 55 2569540
22523 Glutamate Antibody Holtman Jr, J. R., L. J. Marion, and D. F. Speck. "Origin of serotonin-containing projections to the ventral respiratory group in the rat." Neuroscience 37.2 (1990): 541-552. Rat IHC 84 2133358
22523 Glutamate Antibody Atkinson, L., T. F. C. Batten, and J. Deuchars. "P2X 2 receptor immunoreactivity in the dorsal vagal complex and area postrema of the rat." Neuroscience 99.4 (2000): 683-696. Rat IF 54 10974431
22523 Glutamate Antibody Jensen, Patricia, D. James Surmeier, and Dan Goldowitz. "Rescue of cerebellar granule cells from death in weaver NR1 double mutants." The Journal of neuroscience 19.18 (1999): 7991-7998. Mouse IHC 28 10479699
22523 Glutamate Antibody Garrido Sanabria, E. R., et al. "Septal GABAergic neurons are selectively vulnerable to pilocarpine-induced status epilepticus and chronic spontaneous seizures." Neuroscience 142.3 (2006): 871-883. Rat 33 16934946
22523 Glutamate Antibody Salecker, I., and P. Distler. "Serotonin-immunoreactive neurons in the antennal lobes of the American cockroach Periplaneta americana: light-and electron-microscopic observations." Histochemistry 94.5 (1990): 463-473. Cockroach 54 2283309
22523 Glutamate Antibody Catapano, Lisa A., et al. "Specific neurotrophic factors support the survival of cortical projection neurons at distinct stages of development." The Journal of Neuroscience 21.22 (2001): 8863-8872. Mouse ICC 48 11698598
22523 Glutamate Antibody Ackermann, M., et al. "The vertebrate homolog of sulfide-quinone reductase is expressed in mitochondria of neuronal tissues." Neuroscience 199 (2011): 1-12. IHC 4 22067608
22523 Glutamate Antibody Shin, Jennifer J., et al. "Transplanted neuroblasts differentiate appropriately into projection neurons with correct neurotransmitter and receptor phenotype in neocortex undergoing targeted projection neuron degeneration." The Journal of Neuroscience 20.19 (2000): 7404-7416. Mouse IHC 80 11007899

Q & A Wall

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Would you sell the conjugate control (glutamate-KLH) for this antibody?
Question By Mark Miller

Unfortunately we don’t offer the conjugate control for the Glutamate antibody. I apologize for the inconvenience.

Answer By Colleen Hammer on 2013-11-21 15:19:12

MSDS

This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.

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