Produced by Dr. Mark Brownfield, the peptide sequence encoding the rat GLP2R was retrieved from the NCBI protein database and evaluated using GeneRunner software to generate antigen candidates for antibody production. Antibody was generated in rabbits and purified by affinity chromatography against the antigenic peptide. The specificity of the antibody was confirmed by Western blotting and by immunoabsorption controls is the immunohistochemistry procedure (see Nelson et al, Endocrinology 148(5)1954-1962, 2007.
The antiserum demonstrates significant labeling of enteroendocrine cells in the intestinal epithelium, as well as cell bodies of vagal afferents in nodose ganglia of the parasympathetic nervous system. Immunolabeling of Western blot revealed a band of approximately 66 kDa in human and rat tissue.
Photo Description: IHC image of enteroendocrine cells in the intestinal epithelium.
Quantity/Volume: 100 µL
State: LiquidReacts With: : Rat
Alternate Names: anti-GLP2R
RRID: to be determined
|PROD #||ANTIBODY||CITATION||SPECIES REACTIVITY||APPL METHOD||RESEARCH AREA||# CITED BY||PUBMED ID|
|24200||Glucagon-like Protein Receptor (GLP2R) Antibody||Fickbohm, David J., Nadja Spitzer, and Paul S. Katz. "Localization and Activation of Glucagon-Like Peptide-2 Receptors on Vagal Afferents in the Rat." The Endocrine Society doi: 10.1210/en.2006-1232. (2007)||Rat||IHC, WB||71||17234710|
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