Glucagon Antibody

Glucagon Antibody
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Price: $250.00
Product ID : 20076

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Description

The antibody has a proven strong Biotin-Streptavidin/HRP immunostaining at a 1/500-1/1000 dilution in human pancreatic islets. Staining is completely eliminated by pretreatment of the diluted antibody in an excess of glucagon.

Preadsorption of the diluted antibody with an excess of the following substances had no effect on glucagon labeling: secretin, vasoactive intestinal peptide, peptide histidine isoleucine-27, gastric inhibitory polypeptide, rat and human growth hormone releasing hormone and somatostatin.

Photo Description: IHC image of alpha cells in the islets of Langerhans of the rat pancreas staining for glucagon (above) and counterstained with hemotoxylin (below). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for frozen sectioning. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:1000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. 

Glucagon counterstained #20076

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Reacts With: Chick, Human, Mouse, Pig, Rat, Sea Lamprey, Snail, Three-Spined Stickleback (Fish), Turtle

Availability: In Stock

Alternate Names:  anti-glucagon

Gene Symbol / ID, Accession #:  GCG,2641, 280802, 397595

 RRID:  AB_572241

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Publications

PROD # ANTIBODY CITATION SPECIES REACTIVITY APPL METHOD RESEARCH AREA # CITED BY PUBMED ID
20076 Glucagon Antibody Am J Pathol. 2002 Aug;161(2):705-15. A neuroendocrine/small cell prostate carcinoma xenograft-LuCaP 49.True LD1, Buhler K, Quinn J, Williams E, Nelson PS, Clegg N, Macoska JA, Norwood T, Liu A, Ellis W, Lange P, Vessella R. Human, Mouse IHC 21 12163395
20076 Glucagon Antibody Anat Embryol (Berl). 1991;184(5):469-77. An immunocytochemical study of the development of the olfactory system in the three-spined stickleback (Gasterosteus aculeatus L., Teleostei). Honkanen T1, Ekström P. Three-Spined Stickleback (Fish) Stickleback Fish IHC 15 1720599
20076 Glucagon Antibody Rawdon, B. B., and Ann Andrew. "An immunocytochemical survey of endocrine cells in the gastrointestinal tract of chicks at hatching." Cell and tissue research 220.2 (1981): 279-292. Chick IHC 70 6170446
20076 Glucagon Antibody Ku, S. K., and H. S. Lee. "An immunohistochemical study of the pancreatic endocrine cells of the nude mouse, Balb/c-nu/nu." European Journal of Histochemistry 50.1 (2009): 61-68. Mouse 6 16584986
20076 Glucagon Antibody Jong Dae, K. I. M., et al. "Anti-diabetic activity of SMK001, a poly herbal formula in streptozotocin induced diabetic rats: Therapeutic study." Biol. Pharm. Bull 29.3 (2006): 477-482. Rat IHC 17 16508149
20076 Glucagon Antibody Pang, Kevin, Chengeto Mukonoweshuro, and Gordon G. Wong. "Beta cells arise from glucose transporter type 2 (Glut2)-expressing epithelial cells of the developing rat pancreas." Proceedings of the National Academy of Sciences 91.20 (1994): 9559-9563. Rat IHC 133 7937805
20076 Glucagon Antibody PLoS One. 2008;3(10):e3559. doi: 10.1371/journal.pone.0003559. Epub 2008 Oct 29. Beta cells within single human islets originate from multiple progenitors. Scharfmann R1, Xiao X, Heimberg H, Mallet J, Ravassard P. Human IF 19 18958289
20076 Glucagon Antibody DDiabetes. 2006 Jul;55(7):2106-14. Beta-cell deficit due to increased apoptosis in the human islet amyloid polypeptide transgenic (HIP) rat recapitulates the metabolic defects present in type 2 diabetes. Matveyenko AV1, Butler PC. Rat IF diabete 16804082
20076 Glucagon Antibody Watanabe, Tohru, Hirotaka Chikazawa, and Junzo Yamada. "Catecholamine-containing pancreatic islet cells of the domestic fowl." Cell and tissue research 237.2 (1984): 239-244. IHC 10 6383620
20076 Glucagon Antibody Bak, Min-Ji, et al. "Cedrela sinensis leaves suppress oxidative stress and expressions of iNOS and COX-2 via MAPK signaling pathways in RAW 264.7 cells." J. Food Sci. Nutr 14 (2009): 269-276. 5 not found
20076 Glucagon Antibody Ku, Sae-Kwang, et al. "Changes of the gastric endocrine cells in the C57BL/6 mouse after implantation of murine lung carcinoma: An immunohistochemical quantitative study." World J Gastroenterol 2005a 11 (2005): 1317-23. Mouse IHC 5 15761969
20076 Glucagon Antibody Mitra, Sudha Warrier, et al. "Colocalization of Somatostatin Receptor sst5 and Insulin in Rat Pancreatic ?-Cells 1." Endocrinology 140.8 (1999): 3790-3796. Rat IHC 81 10433240
20076 Glucagon Antibody Wang, Gordon G., et al. "Comparison of genetic alterations in neuroendocrine tumors: frequent loss of chromosome 18 in ileal carcinoid tumors." Modern pathology 18.8 (2005): 1079-1087. Human 47 15920555
20076 Glucagon Antibody Naya, Francisco J., et al. "Diabetes, defective pancreatic morphogenesis, and abnormal enteroendocrine differentiation in BETA2/neuroD-deficient mice." Genes & Development 11.18 (1997): 2323-2334. Mouse IHC diabete 825 9308961
20076 Glucagon Antibody Kanji, Murtaza S., Martin G. Martin, and Anil Bhushan. "Dicer1 Is Required to Repress Neuronal Fate During Endocrine Cell Maturation." Diabetes 62.5 (2013): 1602-1611. Mouse IHC 2 23382448
20076 Glucagon Antibody Meier, J. J., et al. "Direct evidence of attempted beta cell regeneration in an 89-year-old patient with recent-onset type 1 diabetes." Diabetologia 49.8 (2006): 1838-1844. Human IF diabete 143 16802132
20076 Glucagon Antibody Ku, S. K., and H. S. Lee. "Distribution and frequency of endocrine cells in the pancreas of the ddY mouse: an immunohistochemical study." European Journal of Histochemistry 49.2 (2009): 125-130. Mouse 9 15967740
20076 Glucagon Antibody Rats, Pregnant Wistar. "Efeitos das drogas anti-retrovirais sobre o metabolismo glic 3 not found
20076 Glucagon Antibody Dagan, Israel, and Yehoshua Earon. "EFFECT OF PARATHYROID HORMONE AND CALCIUM ON LIVER AND KIDNEY ENZYMES Eitan Bogin Department of Biochemistry, Kimron Veterinary Institute, Beit." Proceedings (selected) of the Fourth International Congress on Clinical Enzymology, Washington, DC, USA, July 30-August 2, 1983. Vol. 2. De Gruyter, 1984. IHC parathyroid 0 not found
20076 Glucagon Antibody Cras-Méneur C1, Elghazi L, Czernichow P, Scharfmann R, Diabetes. 2001 Jul;50(7):1571-9. "Epidermal growth factor increases undifferentiated pancreatic embryonic cells in vitro: a balance between proliferation and differentiation" Rat IHC 131 11423478
20076 Glucagon Antibody Le May, Cedric, et al. "Estrogens protect pancreatic ?-cells from apoptosis and prevent insulin-deficient diabetes mellitus in mice." Proceedings of the National Academy of Sciences 103.24 (2006): 9232-9237. Mouse diabete 184 16754860
20076 Glucagon Antibody Castaing, Muriel, et al. "Ex vivo analysis of acinar and endocrine cell development in the human embryonic pancreas." Developmental dynamics 234.2 (2005): 339-345. Human, Mouse 35 16134081
20076 Glucagon Antibody Fourcade, Gwladys, et al. "Fetal pancreas transplants are dependent on prolactin for their development and prevent type 1 diabetes in syngeneic but not allogeneic mice." Diabetes 62.5 (2013): 1646-1655. Mouse IHC diabete 1 23423564
20076 Glucagon Antibody Elson, Amanda ET, et al. "Glucagon signaling modulates sweet taste responsiveness." The FASEB Journal 24.10 (2010): 3960-3969. Mouse 22 20547661
20076 Glucagon Antibody Pak, C. Y., et al. "Human pancreatic islet cell specific 38 kilodalton autoantigen identified by cytomegalovirus-induced monoclonal islet cell autoantibody." Diabetologia 33.9 (1990): 569-572. Human IHC 76 2174808
20076 Glucagon Antibody Buchan, A. M. J., M. Gregor, and E-O. Riecken. "Immunocytochemical characterization of glucagon-immunoreactive cells using monoclonal antibodies to pancreatic glucagon." Histochemistry 87.1 (1987): 79-83. 4 3301754
20076 Glucagon Antibody Cinar, K., and A. Dller. "Immunohistochemical localization of glucagon, substance?P and vasoactive intestinal peptide in gastrointestinal tract mucosa of zander." Journal of fish biology 60.2 (2002): 319-327. 9 not found
20076 Glucagon Antibody Sugitani, Atsushi, James C. Reynolds, and Satoru Todo. "Immunohistochemical study of enteric nervous system after small bowel transplantation in humans." Digestive diseases and sciences 39.11 (1994): 2448-2456. Human 24 7956615
20076 Glucagon Antibody Matveyenko, Aleksey V., et al. "Inconsistent formation and nonfunction of insulin-positive cells from pancreatic endoderm derived from human embryonic stem cells in athymic nude rats." American journal of physiology. Endocrinology and metabolism 299.5 (2010): E713-20. Rat, Human IF Stem Cell 30 20587750
20076 Glucagon Antibody Dheen, S. T., K. Rajkumar, and L. J. Murphy. "Islet cell proliferation and apoptosis in insulin-like growth factor binding protein-1 in transgenic mice." Journal of endocrinology 155.3 (1997): 551-558. Mouse Apoptosis 16 9488000
20076 Glucagon Antibody Butler, Alexandra E., et al. "Marked expansion of exocrine and endocrine pancreas with incretin therapy in humans with increased exocrine pancreas dysplasia and the potential for glucagon-producing neuroendocrine tumors." Diabetes 62.7 (2013): 2595-2604. Human IHC 112 23524641
20076 Glucagon Antibody KOMATSU, SUGURU, et al. "Maternal adrenocortical hormones maintain the early development of pancreatic B cells in the fetal rat." Journal of anatomy 193.4 (1998): 551-557. Rat IHC 7 10029188
20076 Glucagon Antibody Polak, Michel, Rapha, Method for increasing the pool of ngn3+ endocrine progenitor cells and pancreatic endocrine cell mass IHC 0 not found
20076 Glucagon Antibody Czernichow, Paul, et al. "Method of producing human beta cell lines." Human IHC 0 not found
20076 Glucagon Antibody Huang, Hsiang-po, et al. "Neogenesis of ?-cells in adult BETA2/NeuroD-deficient mice." Molecular endocrinology 16.3 (2002): 541-551. Mouse IHC 63 11875114
20076 Glucagon Antibody Alba, Y., et al. "Neuroendocrine Complexes in the Gut of the Snail Helix aspersa." Journal of morphology 199.3 (1989): 313-325. Snail ICC 7 not found
20076 Glucagon Antibody Seglen, Per O., et al. "Neuroendocrine dysdifferentiation and bombesin production in carcinogen-induced hepatocellular rat tumours." Carcinogenesis 10.1 (1989): 21-25. Rat 22 2910524
20076 Glucagon Antibody Sithigorngul, Paisarn, Antony OW Stretton, and Cynthia Cowden. "Neuropeptide diversity in Ascaris: an immunocytochemical study." Journal of Comparative Neurology 294.3 (1990): 362-376. 68 2341616
20076 Glucagon Antibody Ku, Sae Kwang, and Jae Hyun Lee. "Original Articles: Immunohistochemical Study of the Pancreatic Endocrine Cells in the BALB/c mice: An Unique Distributional Pattern of Glucagon." Journal of veterinary science 3.3 (2002): 167-173. Mouse 8 12514327
20076 Glucagon Antibody Georgia, Senta, et al. "p57 and Hes1 coordinate cell cycle exit with self-renewal of pancreatic progenitors." Developmental biology 298.1 (2006): 22-31. Mouse Cell Cycle 94 16899237
20076 Glucagon Antibody Dhawan, Sangeeta, et al. "Pancreatic Beta Cell Identity Is Maintained by DNA Methylation-Mediated Repression of< i< Arx." Developmental cell 20.4 (2011): 419-429. Mouse WB DNA 50 21497756
20076 Glucagon Antibody Ding, Wei-Guang, et al. "Phylogenetic study of serotonin-immunoreactive structures in the pancreas of various vertebrates." Cell and tissue research 263.2 (1991): 237-243. IHC 17 1672509
20076 Glucagon Antibody Meier, Juris J., et al. "Postprandial Suppression of Glucagon Secretion Depends on Intact Pulsatile Insulin Secretion Further Evidence for the Intraislet Insulin Hypothesis." Diabetes 55.4 (2006): 1051-1056. Pig IHC 89 16567528
20076 Glucagon Antibody Yang, Qi, et al. "Requirement of Math1 for secretory cell lineage commitment in the mouse intestine." Science 294.5549 (2001): 2155-2158. Mouse 517 11739954
20076 Glucagon Antibody Elghazi, Lynda, et al. "Role for FGFR2IIIb-mediated signals in controlling pancreatic endocrine progenitor cell proliferation." Proceedings of the National Academy of Sciences 99.6 (2002): 3884-3889. Rat IHC 107 11891329
20076 Glucagon Antibody Rachdi, Latif, Jean-Claude Marie, and Raphael Scharfmann. "Role for VPAC2 receptor-mediated signals in pancreas development." Diabetes 52.1 (2003): 85-92. Rat 12 12502497
20076 Glucagon Antibody COHN, DAVID V., et al. "Selective Localization of the Parathyroid Secretory Protein-I/Adrenal Medulla Chromogranin A Protein Family in a Wide Variety of Endocrine Cells of the Rat*." Endocrinology 114.6 (1984): 1963-1974. Rat IF parathyroid 204 6233131
20076 Glucagon Antibody Elliott, W. M., and J. H. Youson. "Somatostatin concentrations in the pancreatic-intestinal tissues of the sea lamprey,< i> Petromyzon marinus L., at various periods of its life cycle." Comparative Biochemistry and Physiology Part A: Physiology 99.3 (1991): 357-360. Sea Lamprey 11 1678333
20076 Glucagon Antibody Wetzel, Randall K., and William D. Eldred. "Specialized neuropeptide Y-and glucagon-like immunoreactive amacrine cells in the peripheral retina of the turtle." Visual neuroscience 14.05 (1997): 867-877. Turtle 8 9364725
20076 Glucagon Antibody Schulthess, Fabienne T., et al. "Supplemental Data CXCL10 Impairs beta Cell Function and Viability in Diabetes through TLR4 Signaling." diabete 0 19187771
20076 Glucagon Antibody Davidson, Mayer B., et al. "Suppression of sleep-induced growth hormone secretion by anticholinergic agent abolishes dawn phenomenon." Diabetes 37.2 (1988): 166-171. 26 3391341
20076 Glucagon Antibody Gierl, Mathias S., et al. "The zinc-finger factor Insm1 (IA-1) is essential for the development of pancreatic ? cells and intestinal endocrine cells." Genes & development 20.17 (2006): 2465-2478. Mouse IF 95 16951258
20076 Glucagon Antibody Stellrecht, Christine MM, et al. "Tissue-specific and developmental regulation of the rat insulin II gene enhancer, RIPE3, in transgenic mice." Journal of Biological Chemistry 272.6 (1997): 3567-3572. Mouse IHC 23 9013607
20076 Glucagon Antibody J Endocrinol. 2008 Nov;199(2):287-98. doi: 10.1677/JOE-08-0289. Epub 2008 Sep 4. Tumour suppressor menin is essential for development of the pancreatic endocrine cells. Fontanière S1, Duvillié B, Scharfmann R, Carreira C, Wang ZQ, Zhang CX. Mouse IHC 10 18772165
20076 Glucagon Antibody Park, I. S., et al. "Up-regulation of clusterin (sulfated glycoprotein-2) in pancreatic islet cells upon streptozotocin injection to rats." Journal of endocrinology 162.1 (1999): 57-65. Rat WB 25 10396021
20076 Glucagon Antibody Dhawan, Sangeeta, et al. "DNA methylation directs functional maturation of pancreatic β cells." The Journal of clinical investigation 125.7 (2015): 2851. 2 26098213
20076 Glucagon Antibody Gurlo, Tatyana, Peter C. Butler, and Alexandra E. Butler. "Evaluation of immunohistochemical staining for glucagon in human pancreatic tissue." Journal of Histotechnology (2015): 2046023615Y-0000000013. Human 0 not found
20076 Glucagon Antibody Kang, Su Jin, et al. "Fermentation with aquilariae lignum enhances the anti-diabetic activity of green tea in type ii diabetic db/db mouse." Nutrients 6.9 (2014): 3536-3571. Mouse Obesity 2 25207824
20076 Glucagon Antibody Busiah, Kanetee, et al. "Human Pancreas Endocrine Cell Populations and Activating ABCC8 Mutations." Hormone Research in Paediatrics 82.1 (2014): sgmppl. Human IF 1 24941889
20076 Glucagon Antibody Ku, SaeKwang. "StreptozotocinInducedDiabeticRats: TherapeuticStudy." Rat 0 not found
20076 Glucagon Antibody Glendinning, John I., et al. "Taste Responsiveness to Sweeteners Is Resistant to Elevations in Plasma Leptin." Chemical senses 40.4 (2015): 223-231. 0 not defined

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MSDS

This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.

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