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This antibody has been shown to react strongly with human GFAP as well as with GFAP from rat, mouse, guinea pig, hamster, kangaroo, sheep, cat and monkey. Excellent staining results were obtained when rabbit anti-glial fibrillary acidic protein serum was tested.
Photo Description: IHC image of astrocytes staining for GFAP in the rat cortex (above) and human cortex (below). Rat tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Human tissue was formalin fixed and embedded in paraffin with microwave antigen retrieval. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:500 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
Quantity / Volume: 6 mL
Reacts With: Cat, Chick, Chicken, Dog, Ewe, Ferret, Gerbil, Guinea Pig, Hamster, Human, Monkey, Mouse, Pigeon, Rat, Zebra Finch (Bird), Zebrafish
Availability: In Stock
Gene Symbol / ID, Accession #: GFAP, 281189
Alternate Names: anti-GFAP
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This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.Download MSDS