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The ImmunoStar Gastrin Releasing Peptide antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat dorsal horn of spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/1,000-1/2,000 in PBS/0.3% Triton X-100 - Bn/Av-HRP Technique. Staining is completely eliminated by pretreatment of 1 mL of diluted antibody with 50 µg of bombesin.
Photo Description: IHC of at stomach staining for gastrin releasing peptide. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for frozen sectioning. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:2000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen and counterstained with hematoxylin
Quantity / Volume: 100 µL
State: Lyophilized Whole Serum
Reacts With: Chick, Hamster, Human, Mouse, Newt, Pigeon, Quail, Rabbit, Ram (Sheep), Rat, Zacco Platypus (Minnow)
Availability: In Stock
Preabsorption Control: Available at irbiologicals, Catalog # IRCP069. Sequence: Glp-Gln-Arg-Leu-Gly-Asn-Gln-Trp-Ala-Val-Gly-His-Leu-Met-NH
Alternate Names: BN; GRP-10; proGRP; preproGRP; gastrin-releasing peptide, anti-GRP, Bombesin, anti-Bombesin
Gene Symbo: GRP
Entrez Gene: 425213 Chick
Entrez Gene: 100769410 Hamster
Entrez Gene: 2922 Human
Entrez Gene: 225642 Mouse
Entrez Gene: 102093899 Pigeon
Entrez Gene: 107325907 Quail
Entrez Gene: 103349406 Rabbit
Entrez Gene: 443332 Ram (Sheep)
Entrez Gene: 171101 Rat
08/17/2015 - 02:05:23 PM
Jason Zhang, Scientist
University of California-San Diego
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Q & A Wall
I have a question regarding your antibody for Bombesin item 20073. Do you have more details on the epitope that this antibody is detecting or in other words the sequence of the immunogen being used for producing the antibody? We are looking for an antibody that has a different immunogen used than amino acids 14 to 27 epitope.
Question By Fanny Honshoven
Here is the amino acid sequence: Glp-Gln-Arg-Leu-Gly-Asn-Gln-Trp-Ala-Val-Gly-His-Leu-Met-NH
Unfortunately I don’t have any more information on the immunogen other than this sequence.
Answer By Colleen Hammer on 2013-04-09 15:22:55
This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.Download MSDS