Gastrin Releasing Peptide (GRP) / Bombesin Antibody

Gastrin Releasing Peptide (GRP) / Bombesin Antibody
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Price: $275.00
Product ID : 20073

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Description

The ImmunoStar Gastrin Releasing Peptide antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat dorsal horn of spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/1,000-1/2,000 in PBS/0.3% Triton X-100 - Bn/Av-HRP Technique. Staining is completely eliminated by pretreatment of 1 mL of diluted antibody with 50 µg of bombesin.

Photo Description: IHC of at stomach staining for gastrin releasing peptide. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for frozen sectioning. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:2000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen and counterstained with hematoxylin

Host: Rabbit

Quantity / Volume: 100 µL

State: Lyophilized Whole Serum

Reacts With:  Chick, Hamster, Human, Mouse, Newt, Pigeon, Quail, Rabbit, Ram (Sheep), Rat, Zacco Platypus (Minnow)

Availability: In Stock

Preabsorption Control: Available at irbiologicals, Catalog # IRCP069.  Sequence: Glp-Gln-Arg-Leu-Gly-Asn-Gln-Trp-Ala-Val-Gly-His-Leu-Met-NH

Alternate Names:  BN; GRP-10; proGRP; preproGRP; gastrin-releasing peptide, anti-GRP, Bombesin, anti-Bombesin

Gene Symbol / ID, Accession #:  GRP,2922

RRID:
AB_572221

Reviews

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Reviewed by zzhongge@ucsd.edu
08/17/2015 - 02:05:23 PM
Scientist

This is our first time using this antibody (Prod ID: 20073) from ImmmunoStar. It works beautifully in our immunohistochemical assays on mouse dorsal tissues. We used 1:2000 dilutions. It is one of the best ones among similar antibodies we have tested from other vendors. We would like to recommend this item to our colleagues.

Jason Zhang, Scientist
University of California-San Diego

Publications

PROD # ANTIBODY CITATION SPECIES REACTIVITY APPL METHOD RESEARCH AREA # CITED BY PUBMED ID
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Dixon, J. S., P. Y. P. Jen, and J. A. Gosling. "A double?label immunohistochemical study of intramural ganglia from the human male urinary bladder neck." Journal of anatomy 190.1 (1997): 125-134. Human ICC 39 9034888
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Nature. 2007 Aug 9;448(7154):700-3. Epub 2007 Jul 25.A gastrin-releasing peptide receptor mediates the itch sensation in the spinal cord. Sun YG1, Chen ZF. Mouse IF 17653196
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Globus, Morton, and Paul Alles. "A search for immunoreactive substance P and other neural peptides in the limb regenerate of the newt Notophthalmus viridescens." Journal of Experimental Zoology 254.2 (1990): 165-176. Newt 18 1693394
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Smith, V. M., et al. "Altered photic and non-photic phase shifts in 5-HT< 1A< receptor knockout mice." Neuroscience 157.3 (2008): 513-523. Mouse 25 18930788
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Favara, Blaise E., and Harold Moores. "Anitschkow nuclear structure: a study of pediatric hearts." Fetal and Pediatric Pathology 7.2 (1987): 151-164. 6 2821523
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Hernanz, Angel. "Characterization and distribution of bombesin-like peptides in the rat brain and gastrointestinal tract." Biochemistry and Cell Biology 68.9 (1990): 1142-1145. Rat RIA 21 2257118
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody LeSauter, Joseph, et al. "Circadian trafficking of calbindin-ir in fibers of SCN neurons." Journal of biological rhythms 24.6 (2009): 488-496. Hamster ICC 4 19926808
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Jen, P. Y. P., J. S. Dixon, and J. A. Gosling. "Development of peptide?containing nerves in the human fetal vas deferens and seminal vesicle." British journal of urology 75.3 (1995): 378-385. Human 19 7735805
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Stein, Brenda, and Ann Andrew. "Differentiation of endocrine cells in chick allantoic epithelium combined with pancreatic mesenchyme." Cell differentiation and development 26.3 (1989): 173-180. Chick 12 2475227
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Bean JA, Brody AS, Nogee LM, Trapnell BC, Langston C; Pathology Cooperative Group, Albright EA, Askin FB, Baker P, Chou PM, Cool CM, Coventry SC, Cutz E, Davis MM,Deutsch GH1, Young LR, Deterding RR, Fan LL, Dell SD, Dishop MK, Galambos C, Patterson K, Travis WD, Wert SE, White FV; ChILD Research Co-operative., Am J Respir Crit Care Med. 2007 Dec 1;176(11):1120-8. Epub 2007 Sep 20. "Diffuse lung disease in young children: application of a novel classification scheme" Human IHC 17885266
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Karatsoreos, Ilia N., et al. "Diurnal regulation of the gastrin?releasing peptide receptor in the mouse circadian clock." European Journal of Neuroscience 23.4 (2006): 1047-1053. Mouse 36 16519669
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Aalto, Yan, et al. "Does bombesin-like peptide mediate radiation-induced anorexia and satiety?." Acta Oncologica 38.8 (1999): 1099-1102. 3 10665769
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Westlund, K. N., et al. "Effects of nerve growth factor and acetyl-L-carnitine arginyl amide on the human neuronal line HCN-1A." International journal of developmental neuroscience 10.5 (1992): 361-373. Human 29 1283485
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Rizk, Sherif L., et al. "Elevated creatine phosphokinase MB in a patient with neuroendocrine carcinoma of the colon Tumor Markers 5 3359901
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Au, N. H. C., et al. "Evaluation of immunohistochemical markers in non?small cell lung cancer by unsupervised hierarchical clustering analysis: a tissue microarray study of 284 cases and 18 markers." The Journal of pathology 204.1 (2004): 101-109. Human IHC cancer 102 15307143
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Houben, Herlinde, and Carl Denef. "Evidence for the Presence of Gastrin-Releasing Peptide Immunoreactivity in Rat Anterior Pituitary Corticotrophs and Lactotrophs, AtT20 Cells, and GH3 Cells: Failure to Demonstrate Participation in Local Control of Hormone Release*." Endocrinology 128.6 (1991): 3208-3218. Rat 16 1645264
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Wang, Dashou, Herman Yeger, and Ernest Cutz. "Expression of gastrin-releasing peptide receptor gene in developing lung." American journal of respiratory cell and molecular biology 14.5 (1996): 409-416. 49 8624245
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Popler, J., et al. "Familial neuroendocrine cell hyperplasia of infancy." Pediatric pulmonology 45.8 (2010): 749-755. Human IHC 18 23787483
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Trandaburu, T., S. Syed Ali, and I. Trandaburu. "Granin proteins (chromogranin A and secretogranin II C23-3 and C26-3) in the intestine of reptiles." Annals of Anatomy-Anatomischer Anzeiger 181.3 (1999): 261-268. 10 10363108
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Multidifferentiated, Protooncogenes Induce. "Human Bronchial Epithelial Cells Transformed by the c." CANCER RESEARCH 51 (1991): 3793-3801. Human ICC 0 1712250
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Luckensmeyer, Greta B., and Janet R. Keast. "Immunohistochemical characterisation of viscerofugal neurons projecting to the inferior mesenteric and major pelvic ganglia in the male rat." Journal of the autonomic nervous system 61.1 (1996): 6-16. Rat 33 8912248
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Lee, J. H., et al. "Immunohistochemical study of the gastrointestinal endocrine cells in the Korean aucha perch." Journal of fish biology 65.1 (2004): 170-181. 25 not found
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Ku, S. K., J. H. Lee, and H. S. Lee. "Immunohistochemical study on the endocrine cells in gut of the stomachless teleost, Zacco platypus (Cyprinidae)." Anatomia, histologia, embryologia 33.4 (2004): 212-219. Zacco Platypus (Minnow) 26 15239812
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Neuropharmacology. 2010 Jan;58(1):277-85. doi: 10.1016/j.neuropharm.2009.06.011. Epub 2009 Jun 21. Investigating the role of substance P in photic responses of the circadian system: individual and combined actions with gastrin-releasing peptide. Sterniczuk R1, Colijn MA, Nunez M, Antle MC. Hamster IF 19540856
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Berk, Mitchell L., and Stacy E. Smith. "Local and commissural neuropeptide?containing projections of the nucleus of the solitary tract to the dorsal vagal complex in the pigeon." Journal of Comparative Neurology 347.3 (1994): 369-396. Pigeon IF 15 7529775
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Martinez, Alfredo, et al. "Localization of amidating enzymes (PAM) in rat gastrointestinal tract." Journal of Histochemistry & Cytochemistry 41.11 (1993): 1617-1622. Rat IHC 25 8409369
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Linnoila, R. Ilona, et al. "Long term selective culture of hamster pulmonary endocrine cells." The Anatomical Record 236.1 (1993): 231-240. Hamster 13 8389531
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Wang, Dashou, et al. "NADPH-oxidase and a hydrogen peroxide-sensitive K+ channel may function as an oxygen sensor complex in airway chemoreceptors and small cell lung carcinoma cell lines." Proceedings of the National Academy of Sciences 93.23 (1996): 13182-13187. Human, Rabbit IHC 170 8917565
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Antle, Michael C., Joseph LeSauter, and Rae Silver. "Neurogenesis and ontogeny of specific cell phenotypes within the hamster suprachiasmatic nucleus." Developmental brain research 157.1 (2005): 8-18. Hamster ICC 16 15939080
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Chott, Andreas, et al. "Neuron specific enolase demonstration in the diagnosis of a solid-cystic (papillary cystic) tumour of the pancreas." Virchows Archiv A 410.5 (1987): 397-402. 37 3103322
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Sithigorngul, Paisarn, Antony OW Stretton, and Cynthia Cowden. "Neuropeptide diversity in Ascaris: an immunocytochemical study." Journal of Comparative Neurology 294.3 (1990): 362-376. 68 2341616
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Miyakawa, Kazuko, et al. "ORL1 receptor-mediated down-regulation of mPER2 in the suprachiasmatic nucleus accelerates re-entrainment of the circadian clock following a shift in the environmental light/dark cycle." Neuropharmacology 52.3 (2007): 1055-1064. Mouse IHC 5 17196226
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Warren, William H., et al. "Paragangliomas of the head and neck: ultrastructural and immunohistochemical analysis." Ultrastructural pathology 8.4 (1985): 333-343. 39 not found
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Deterding, Robin R., et al. "Persistent tachypnea of infancy is associated with neuroendocrine cell hyperplasia." Pediatric pulmonology 40.2 (2005): 157-165. Human 78 15965897
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Karatsoreos, Ilia N., et al. "Phenotype matters: identification of light-responsive cells in the mouse suprachiasmatic nucleus." The Journal of neuroscience 24.1 (2004): 68-75. Mouse ICC 89 14715939
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Marchevsky, A. M., and J. Kleinerman. "Quantitative and immunocytochemical studies of APUD cells in airways and gut." Lung 161.1 (1983): 67-76. 11 6188931
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Akiyama, Tasuku, et al. "Roles of glutamate, substance P, and gastrin-releasing peptide as spinal neurotransmitters of histaminergic and nonhistaminergic itch." PAIN Mouse IHC 2 24041961
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Keast, J. R., and H-C. Chiam. "Selective association of nerve fibres immunoreactive for substance P or bombesin with putative cholinergic neurons of the male rat major pelvic ganglion." Cell and tissue research 278.3 (1994): 589-594. Rat 10 7531621
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Liu, Qin, et al. "Sensory neuron-specific GPCR Mrgprs are itch receptors mediating chloroquine-induced pruritus." Cell 139.7 (2009): 1353-1365. Mouse IHC 165 20004959
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Neuroscience. 2010 Jan 13;165(1):16-27. doi: 10.1016/j.neuroscience.2009.09.068. Epub 2009 Sep 30. Serotonergic potentiation of photic phase shifts: examination of receptor contributions and early biochemical/molecular events. Smith VM1, Hagel K, Antle MC. Mouse IF 19799970
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Vittoria, A., et al. "Serotonin-, somatostatin-and chromogranin A-containing cells of the urethro-prostatic complex in the sheep. An immunocytochemical and immunofluorescent study." Journal of anatomy 171 (1990): 169. Ram / Sheep IF 26 1981998
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Kramer, B. E. V. E. R. L. E. Y., et al. "The effect of pancreatic mesenchyme on the differentiation of endocrine cells from gastric endoderm." Development 100.4 (1987): 661-671. Quail ICC 28 3443051
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Andrew, Ann, Beverley Kramer, and B. B. Rawdon. "The embryonic origin of endocrine cells of the gastrointestinal tract." General and comparative endocrinology 47.2 (1982): 249-265. 30 7095418
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Fleming, Michael S., et al. "The majority of dorsal spinal cord gastrin releasing peptide is synthesized locally whereas neuromedin B is highly expressed in pain-and itch-sensing somatosensory neurons." Mol Pain 8.1 (2012): 52. Mouse IHC 20 22776446
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody J Clin Invest. 2012 Jun;122(6):2195-207. doi: 10.1172/JCI45414. Epub 2012 May 8. TLR3 deficiency impairs spinal cord synaptic transmission, central sensitization, and pruritus in mice.Liu T1, Berta T, Xu ZZ, Park CK, Zhang L, Lü N, Liu Q, Liu Y, Gao YJ, Liu YC, Ma Q, Dong X, Ji RR. Mouse IF 22565312
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Montuenga, L. M., et al. "Ultrastructure and immunocytochemistry of endocrine cells in the midgut of the desert locust, Schistocerca gregaria (Forskal)." Cell and tissue research 258.3 (1989): 577-583. 49 not found
20073 GRP (Gastrin Releasing Peptide) / Bombesin Antibody Obara, Ilona, et al. "Inhibition of the Mammalian Target of Rapamycin Complex 1 (mTORC1) signaling pathway reduces itch behaviour in mice." Pain (2015). Mouse Pain 0 25906350

Q & A Wall

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I have a question regarding your antibody for Bombesin item 20073. Do you have more details on the epitope that this antibody is detecting or in other words the sequence of the immunogen being used for producing the antibody? We are looking for an antibody that has a different immunogen used than amino acids 14 to 27 epitope.
Question By Fanny Honshoven

Here is the amino acid sequence: Glp-Gln-Arg-Leu-Gly-Asn-Gln-Trp-Ala-Val-Gly-His-Leu-Met-NH

Unfortunately I don’t have any more information on the immunogen other than this sequence.

Answer By Colleen Hammer on 2013-04-09 15:22:55

MSDS

This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.

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