DBH (Dopamine-beta-Hydroxylase) Antibody

DBH positive neurons in rat brainstem
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Price: $375.00
Product ID : 22806

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Description

The antibody has a proven strong biotin-streptavidin/HRP staining at a 1/2000 - 1/4000 dilution in rat brainstem, cerebellum and adrenal medulla. 

The above image is of DBH positive neurons in rat brainstem,  vibratome sectioned, ABC / DAB detection.

Using Western blot of purified DBH the antiserum detects a triplet at approximately 72-74 kD. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.

Photo Description: IHC image of neurons staining for DBH in the rat brainstem. The tissue was fixed with 4% formaldehyde/0.05% glutaraldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:4000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Species Reactivity: Bird, Cat, Ferret, Finch, Guinea Pig, Hamster, Hatchetfish, Human, Ilyanassa Obsoleta (Sea Snail), Monkey, Mouse, Pig, Quail, Rat, Sparrow, Starling, Steer (Cattle), Turkey, Turtle, Zebra Finch

Availability: In Stock

Alternate Names:  DBM; DOPO; Dopamine beta-monooxygenase, anti-DBH

RRID:  AB_572229

Immunogen: Bovine DBH

Gene Symbol:  DBH

Entrez Gene ID: 280758

Reviews

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Reviewed by user
11/12/2015 - 09:06:22 AM
DBH Antibody Worked Beautifully

Mouse hearts were perfused with PBS (pH 7.4) at RT, followed by cold fixative (4% paraformaldehyde + 0.2% picric acid in PBS). Tissues were fixed again for 2h at 4°C in cold 20% sucrose/PBS, and sectioned in a microtome. Slides was wrapped in aluminum foil and stored at −20°C.

Slide-mounted heart tissue sections were washed 3 x 5 min in 0.1M PBS, permeabilized with 0.4% Triton X-100 plus 0.5% BSA for 20 min. Tissues were then incubated overnight with DBH antibody (1:1000 dilution), washed four times with 0.1M PBS (10 min each), permeabilized with 0.4% Triton X-100 + 0.5% BSA, and incubated for 1.5h with a secondary antibody. After washing, labeled tissue sections were viewed under a confocal microscope system.

Labelled tissue sections showed clear labeling while the negative control sections (with our DBH antibody) showed only background fluorescence. This DBH antibody worked beautifully in our experiments!

Jason Zhang, Project Scientist
University of California- San Diego

Reviewed by kellysa7
06/04/2015 - 08:32:05 AM
Very nice antibody

We used 22806 in rat tissue.Brains were fixed using 4% paraformaldehyde, saturated in 30% sucrose and stored at -80. Brains were sectioned at 40 um using a freezing/sliding microtome and sections were stored in cryoprotectant at -20.
The antibody clearly labeled fiber innervation in the cortical areas and innervation was easily measured via stereology. Brains were incubated overnight at RT at 1:500 in TBST +1% Goat Serum after blocking in 10% Serum in TBST.
Vector biotinylated anti-rabbit was then used at a 1:500 dilution in TBST. After 1 hr ABC incubation, antibody was detected with DAB Nickel with a 10 minute development.

Submitted by:
Sarah Kelly
PhD Candidate
Department of Translational Science and Molecular Medicine
Michigan State University



Reviewed by user
02/27/2014 - 02:02:15 PM
Very happy with the results

We used rabbit anti-bovine DBH (#22806) in an ICC Zenk protocol with sectioned zebra finch brain tissue and were very happy with the results. The antibody provided clear staining with minimal background. The antibody clearly labeled neuron bodies as well as tracks.

Our protocol followed Sockman & Salvante 2007. Zebra finch brains were fixed using 4% paraformaldehyde, saturated in 30% sucrose and stored at -80. Brains were sectioned at 40 um using a cryostat and sections were stored in cryoprotectant at -20.

Primary

1. 3x5 min wash in PBS
2. 15 min incubation in 0.1% sodium borohydride
3. 3x5 min wash in PBS
4. 30 min incubation in 0.5% H2O2 in PBS
5. 3x5 min was in 0.3% PBST
6. 1 hr incubation in 20% normal goat serum
7. 15 min incubation in Aviden solution
8. 3x5 min wash in 0.3% PBST
9. 15 min incubation in Biotin
10. 3x5 min wash in 0.3% PBST
11. 48 hr incubation at 4˚C at 1:16,000 in PBSTN

Secondary

1. 1 hour incubation in secondary antibody at RT
2. Visualize using ABC kit and DAB
3. Dehydrate and coverslip

Submitted by:

Kendra Sewall, PhD
Assistant Professor
Biological Sciences, Virginia Tech

Reviewed by user
11/15/2012 - 11:33:39 AM
Antibody Worked Very Well

We tried the rabbit anti-dopamine-beta-hydroxylase antibody in ferret tissues, more specifically on brainstem tissues. Ferrets were perfused with saline followed by 4% paraformaldehyde and postfixed for 12 hours.

We used 1:500 dilution of DBH antibody and stained with Alexa 594 as a secondary antibody. The citation of the antibody has been published in the following article:

Allergic lung inflammation affects central noradrenergic control of cholinergic outflow to the airways in ferrets. Wilson CG, Akhter S, Mayer CA, Kc P, Balan KV, Ernsberger P, Haxhiu MA. J Appl Physiol. 2007 Dec;103(6):2095-104. Epub 2007 Sep 13. PMID:17872402[PubMed]

Submitted by:

Prabha Kc, Ph.D.
Assistant Professor
Case Western Reserve University
Department of Pediatrics
Cleveland, OH

Reviewed by user
09/14/2012 - 11:22:04 AM
Detected small fibers with very little background

Animals were briefly anesthetized with pentobarbital (200mg/kg) followed by transcardial perfusion with 200 ml heparinized saline and 500 ml Zamboni fixative. Brains were then removed and stored in 30% sucrose until further processing.

IHC

Brains were frozen and cut at 50 µm thick sections using a cryostat.

Free-floating sections were first washed with PBS and then pretreated with a solution of 50% methanol, 0.3% hydrogen peroxide in PBS for 1 hour.

Blocking Solution: 1% triton-X 100, 2% fetal bovine serum in .1 M PBS for at least 1 hour at room temperature.

Dilutent for primary and secondary antibodies: 0.5% triton-X 100, 2% fetal bovine serum in .1 M PBS at

Primary dilution 1:5000 overnight at 4°C
Secondary Alexa 488 (Life Technologies) 1:500 in dilutent for 1 hour

Sections were washed thoroughly in .1 M PBS following detection, coverslipped and mounted with Vectashield mounting medium (Vector Labs)


This antibody detected small fibers within the cortex, central amygdala and various nuclei of the thalamus and hypothalamus with very little background.

Note: The time which animals spend under anesthesia will affect the level of expression in the thalamus/hypothalamus regions.

Review submitted by:

Vanessa M. Kainz
Research Associate
Department of Anesthesia and Critical Care Beth Israel Deaconess Medical Center

Reviewed by user
04/23/2012 - 02:32:02 PM
High quality antibody

I used the DBH antibody and the results showed very nice staining and no background.

Zebra finch brain tissue
Flash frozen and stored at -80 degrees
Sectioned at 20 um on cryostat and placed on superfrost plus slides.
Slides stored at -80 degrees until processing.
4% paraformaldehyde -15 min @ RT (room temperature)
3 X 5 min washes in 1X PBS @ RT
0.9% H2O2 in methanol-15 min @RT
3 X 5 min washes in 1XPBS @ RT
10% normal goat serum - 1 hour @ RT

Primary antibody incubation: overnight @ 4 degrees
1 ul/ml antibody
10% normal goat serum
1XPBS-TritonX

Secondary antibody - 90 min @ RT

Visualize using ABC kit (Vector) and DAB
Dehydrate and coverslip.


Review submitted by:

Juli S. Wade, PhD
Yu-Ping Tang, PhD
Neuroscience Program
Michigan State University

Publications

PROD # ANTIBODY CITATION SPECIES REACTIVITY APPL METHOD RESEARCH AREA # CITED BY PUBMED ID
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Halliday, G. M., and E. M. McLachlan. "A comparative analysis of neurons containing catecholamine-synthesizing enzymes and neuropeptide Y in the ventrolateral medulla of rats, guinea-pigs and cats." Neuroscience 43.2 (1991): 531-550. Rat, Guinea Pig, Cat IHC 45 1681467
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Dixon, J. S., P. Y. P. Jen, and J. A. Gosling. "A double?label immunohistochemical study of intramural ganglia from the human male urinary bladder neck." Journal of anatomy 190.1 (1997): 125-134. Human ICC 39 9034888
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Castelino, Christina B., and Gregory F. Ball. "A role for norepinephrine in the regulation of context?dependent ZENK expression in male zebra finches (Taeniopygia guttata)." European Journal of Neuroscience 21.7 (2005): 1962-1972. Finch IHC 59 15869489
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Shetty, Ashok Kumar, Gomathy Gopinath, and P. N. Tandon. "Adrenal medullary autografts in anterior eye chamber, lateral ventricle and striatum of adult rats: a long term study." Neural Plasticity 2.3-4 (1991): 175-191. Rat 3 1685897
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Green BT1, Lyte M, Chen C, Xie Y, Casey MA, Kulkarni-Narla A, Vulchanova L, Brown DR., Am J Physiol Gastrointest Liver Physiol. 2004 Dec;287(6):G1238-46. "Adrenergic modulation of Escherichia coli O157:H7 adherence to the colonic mucosa" Pig IF 15534374
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Balan, Paul Ernsberger, and Musa A. Haxhiu. "Allergic lung inflammation affects central noradrenergic." J Appl Physiol 103 (2007): 2095-2104. Ferret IHC 0 17872402
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Behrens, Edward G., Brett R. Schofield, and Ann M. Thompson. "Aminergic projections to cochlear nucleus via descending auditory pathways." Brain research 955.1 (2002): 34-44. 13 12419519
22806 DBH (Dopamine-beta-Hydroxylase) Antibody He, Gonghao, et al. "Arrhythmogenic effect of sympathetic histamine in mouse hearts subjected to acute ischemia." Molecular Medicine 18.1 (2012). Mouse IHC 6 21989948
22806 DBH (Dopamine-beta-Hydroxylase) Antibody J Neuroimmunol. 2007 Apr;185(1-2):20-8. Epub 2007 Feb 21. Autonomic neurotransmitters modulate immunoglobulin A secretion in porcine colonic mucosa. Schmidt LD1, Xie Y, Lyte M, Vulchanova L, Brown DR.J Neuroimmunol. 2007 Apr;185(1-2):64-74. Epub 2007 Mar 23. Anatomical evidence for enteric neuroimmune interactions in Peyer's patches. Vulchanova L1, Casey MA, Crabb GW, Kennedy WR, Brown DR. Pig IHC 17320195
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Haidan, A., et al. "Basal Steroidogenic Activity of Adrenocortical Cells Is Increased 10-Fold by Coculture with Chromaffin Cells 1." Endocrinology 139.2 (1998): 772-780. Steer (Cattle) IHC 60 9449652
22806 DBH (Dopamine-beta-Hydroxylase) Antibody J Neurotrauma. 2004 Oct;21(10):1415-30. Basic fibroblast growth factor promotes neuronal survival but not behavioral recovery in the transected and Schwann cell implanted rat thoracic spinal cord. Meijs MF1, Timmers L, Pearse DD, Tresco PA, Bates ML, Joosten EA, Bunge MB, Oudega M. Rat IF 15672632
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Pawlisch, Benjamin A., et al. "Behavioral indices of breeding readiness in female European starlings correlate with immunolabeling for catecholamine markers in brain areas involved in sexual motivation." General and comparative endocrinology 179.3 (2012): 359-368. Bird (Starling) 2 22999823
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Neuroscience. 1992 Jul;49(1):33-44. Behaviour-dependent changes in acetylcholine release in normal and graft-reinnervated hippocampus: evidence for host regulation of grafted cholinergic neurons. Nilsson OG1, Björklund A. 41 1407550
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Dvoryanchikov, Gennady, Seth M. Tomchik, and Nirupa Chaudhari. "Biogenic amine synthesis and uptake in rodent taste buds." Journal of Comparative Neurology 505.3 (2007): 302-313. Mouse 47 17879273
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Cassam, A. K., I. J. Llewellyn-Smith, and L. C. Weaver. "Catecholamine enzymes and neuropeptides are expressed in fibres and somata in the intermediate gray matter in chronic spinal rats." Neuroscience 78.3 (1997): 829-841. Rat 33 9153661
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Mundorf, Michelle L., et al. "Catecholamine release and uptake in the mouse prefrontal cortex." Journal of neurochemistry 79.1 (2001): 130-142. Mouse 81 11595765
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Vaughan, C. H., Y. B. Shrestha, and T. J. Bartness. "Characterization of a novel melanocortin receptor-containing node in the SNS outflow circuitry to brown adipose tissue involved in thermogenesis." Brain research 1411 (2011): 17-27. Hamster IHC 6 21802070
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Hoard, Jennifer L., et al. "Cholinergic neurons of mouse intrinsic cardiac ganglia contain noradrenergic enzymes, norepinephrine transporters, and the neurotrophin receptors tropomyosin-related kinase A and p75." Neuroscience 156.1 (2008): 129-142. Mouse IHC 27 18674600
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Jen, P. Y. P., J. S. Dixon, and J. A. Gosling. "Colocalisation of neuropeptides, nitric oxide synthase and immunomarkers for catecholamines in nerve fibres of the adult human vas deferens." Journal of anatomy 195.4 (1999): 481-489. Human IHC 7 10634688
22806 DBH (Dopamine-beta-Hydroxylase) Antibody von Bartheld, Christopher S., and Mark Bothwell. "Development and distribution of noradrenergic and cholinergic neurons and their trophic phenotypes in the avian ceruleus complex and midbrain tegmentum." Journal of Comparative Neurology 320.4 (1992): 479-500. IHC 28 1321173
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Jen, P. Y., and J. S. Dixon. "Development of peptide-containing nerves in the human fetal prostate gland." Journal of anatomy 187.Pt 1 (1995): 169. Human 32 7591978
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Pangestiningsih TW1, Hendrickson A, Sigit K, Sajuthi D, Nurhidayat, Bowden DM, Brain Res. 2009 Jul 14;1280:23-32. doi: 10.1016/j. brainres.2009.05.028. Epub 2009 May 19. "Development of the area postrema: an immunohistochemical study in the macaque" Monkey IF 19460361
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Dickinson, Amanda JG, and Roger P. Croll. "Development of the larval nervous system of the gastropod Ilyanassa obsoleta." Journal of Comparative Neurology 466.2 (2003): 197-218. Ilyanassa Obsoleta (Sea Snail) 58 14528448
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Brain Res Dev Brain Res. 2003 Dec 19;146(1-2):1-13. Direct cell-cell contact required for neurotrophic effect of chromaffin cells on neural progenitor cells. Schumm MA1, Castellanos DA, Frydel BR, Sagen J. 20 14643006
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Vis Neurosci. 2002 May-Jun;19(3):321-33. Distribution and origin of the catecholaminergic innervation in the amphibian mesencephalic tectum. Sánchez-Camacho C1, Marín O, González A. IHC 7 12392181
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Goldsmith, S. K., and J. N. Joyce. "Dopamine D2 receptors are organized in bands in normal human temporal cortex." Neuroscience 74.2 (1996): 435-451. Human 43 8865195
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Gore, Andrea C., Youichi Saitoh, and Ei Terasawa. "Effects of adrenal medulla transplantation into the third ventricle on the onset of puberty in female rhesus monkeys." Experimental neurology 140.2 (1996): 172-183. Monkey 8 8690060
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Bailhache, T., C. Surlemont, and Jacques Balthazart. "Effects of neurochemical lesions of the preoptic area on male sexual behavior in the Japanese quail." Brain research bulletin 32.3 (1993): 273-283. Quail 9 8374807
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Schumm, Michael A., et al. "Enhanced viability and neuronal differentiation of neural progenitors by chromaffin cell co-culture." Developmental brain research 137.2 (2002): 115-125. 31 12220703
22806 DBH (Dopamine-beta-Hydroxylase) Antibody LeBlanc MM1, Goode CT, MacDougall-Shackleton EA, Maney DL, Brain Res. 2007 Sep 26;1171:93-103. Epub 2007 Jul 27. "Estradiol modulates brainstem catecholaminergic cell groups and projections to the auditory forebrain in a female songbird" Sparrow IHC 17764666
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Matragrano, Lisa L., et al. "Estradiol?dependent catecholaminergic innervation of auditory areas in a seasonally breeding songbird." European Journal of Neuroscience 34.3 (2011): 416-425. Rat IF 19 21714815
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Andrews, Melissa R., and Dennis J. Stelzner. "Evaluation of olfactory ensheathing and Schwann cells after implantation into a dorsal injury of adult rat spinal cord." Journal of neurotrauma 24.11 (2007): 1773-1792. Rat 46 18001205
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Physiol Behav. 2008 Sep 3;95(1-2):258-66. doi: 10.1016/j.physbeh.2008.06.009. Epub 2008 Jun 21.Evidence that dopamine within motivation and song control brain regions regulates birdsong context-dependently. Heimovics SA1, Riters LV.6 Starling IHC 18619478
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Riters, Lauren V., and Benjamin A. Pawlisch. "Evidence that norepinephrine influences responses to male courtship song and activity within song control regions and the ventromedial nucleus of the hypothalamus in female European starlings." Brain research 1149 (2007): 127-140. Starling 22 17379191
22806 DBH (Dopamine-beta-Hydroxylase) Antibody J Comp Neurol. 2010 Jul 1;518(13):2538-53. doi: 10.1002/cne.22351. Expression of the embryonal isoform (0N/3R) of the microtubule-associated protein tau in the adult rat central nervous system.Bullmann T1, Härtig W, Holzer M, Arendt T. Rat IF 20503426
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Espejo, Emilio Fernandez, et al. "Functional regeneration in a rat Parkinson's model after intrastriatal grafts of glial cell line-derived neurotrophic factor and transforming growth factor ?1-expressing extra-adrenal chromaffin cells of the zuckerkandl's organ." The Journal of Neuroscience 21.24 (2001): 9888-9895. Rat IHC, IF parkinson 33 11739596
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Kawasaki, Hiroshi, et al. "Generation of dopaminergic neurons and pigmented epithelia from primate ES cells by stromal cell-derived inducing activity." Proceedings of the National Academy of Sciences 99.3 (2002): 1580-1585. Monkey IF 415 11818560
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Stubbusch, Jutta, et al. "Generation of the tamoxifen?inducible DBH?Cre transgenic mouse line DBH?CT." genesis 49.12 (2011): 935-941. Mouse 4 21634003
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Bethea, Cynthia L., et al. "Hypothalamic KISS1 Expression, Gonadotrophin?Releasing Hormone and Neurotransmitter Innervation Vary with Stress and Sensitivity in Macaques." Journal of neuroendocrinology 26.5 (2014): 267-281. Monkey IHC 0 24617839
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Zaccone, Giacomo, et al. "Immunolocalization of neurotransmitter-synthesizing enzymes and neuropeptides with associated receptors in the photophores of the hatchetfish,Argyropelecus hemigymnus Cocco, 1829." Acta histochemica 113.4 (2011): 457-464. Hatchetfish 0 20546867
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Brain Res. 2003 Oct 31;989(1):117-21. Interaction between catecholaminergic terminals and urocortinergic neurons in the Edinger-Westphal nucleus in the rat. Gaszner B1, Kozicz T. Rat IHC 8 14519518
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Cantor, Tom, Patty Glowacki, and Steve Scheibel. "Large differences between commercial intact PTH assays." J Am Soc Nephrol 14 (2003): 688A. IHC 4 not found
22806 DBH (Dopamine-beta-Hydroxylase) Antibody J Neurosci. 1998 May 15;18(10):3803-15. Long-distance axonal regeneration in the transected adult rat spinal cord is promoted by olfactory ensheathing glia transplants. Ramón-Cueto A1, Plant GW, Avila J, Bunge MB. Rat 673 9570810
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Wernli, Gwenaelle, et al. "Macrophage depletion suppresses sympathetic hyperinnervation following myocardial infarction." Basic research in cardiology 104.6 (2009): 681-693. Rat IHC 43 19437062
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Cantor, Thomas L., and Ping Gao. "Methods for differentiating and monitoring parathyroid and bone status related diseases." U.S. Patent No. 7,943,323. 17 May 2011. parathyroid 2 not found
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Chen, Chunsheng, et al. "Mucosally-directed adrenergic nerves and sympathomimetic drugs enhance non-intimate adherence of Escherichia coli O157: H7 to porcine cecum and colon." European journal of pharmacology 539.1 (2006): 116-124. Mouse, Pig 31 16687138
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Jeon, Beom S. "Noradrenergic Changes in an Experimental Model of Parkinsonism Using 6-hydroxydopamine." Journal of the Korean Neurological Association 14.2 (1996): 448-454. IHC parkinson 0 22138505
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Antonopoulos, John, et al. "Noradrenergic innervation of the developing and mature septal area of the rat." Journal of Comparative Neurology 476.1 (2004): 80-90. Rat ICC 18 15236468
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Latsari, Maria, et al. "Noradrenergic innervation of the developing and mature visual and motor cortex of the rat brain: a light and electron microscopic immunocytochemical analysis." Journal of Comparative Neurology 445.2 (2002): 145-158. Rat ICC 29 11891659
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Castelino, Christina B., Bettina Diekamp, and Gregory F. Ball. "Noradrenergic projections to the song control nucleus area X of the medial striatum in male zebra finches (Taeniopygia guttata)." Journal of Comparative Neurology 502.4 (2007): 544-562. Finch ICC 29 17394158
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Wade, Juli, et al. "Norepinephrine inhibition in juvenile male zebra finches modulates adult song quality." Brain research bulletin 90 (2013): 132-136. Zebra Finch (Bird) IHC 1 23160069
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Brosnahan, Amanda J., et al. "Norepinephrine potentiates proinflammatory responses of human vaginal epithelial cells." Journal of neuroimmunology 259.1 (2013): 8-16. Human 0 23571017
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Stewart, Graham R., Roland N. Perry, and Denis J. Wright. "Occurrence of dopamine in Panagrellus redivivus and Meloidogyne incognita." Nematology 3.8 (2001): 843-848. 11 not found
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Lin, Bryan T-Y., et al. "Oncocytic adrenocortical neoplasms: a report of seven cases and review of the literature." The American journal of surgical pathology 22.5 (1998): 603-614. 110 9591731
22806 DBH (Dopamine-beta-Hydroxylase) Antibody PLoS One. 2011;6(5):e20131. doi: 10.1371/journal.pone.0020131. Epub 2011 May 23.Own song selectivity in the songbird auditory pathway: suppression by norepinephrine.Poirier C1, Boumans T, Vellema M, De Groof G, Charlier TD, Verhoye M, Van der Linden A, Balthazart J. Zebra Finch (Bird) IF 21625389
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Belfry, C. Susan, and F. Brian M. Cowan. "Peptidergic and adrenergic innervation of the lachrymal gland in the euryhaline turtle, Malaclemys terrapin." Journal of Experimental Zoology 273.5 (1995): 363-375. Turtle 8 8576694
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Zhang, Xiaoli, et al. "Pontine norepinephrine defects inMecp2- null mice involve deficient expression of dopamine ?-hydroxylase but not a loss of catecholaminergic neurons." Biochemical and biophysical research communications 394.2 (2010): 285-290. Mouse 11 20193660
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Brock, J. A., D. J. Handelsman, and J. R. Keast. "Postnatal androgen deprivation dissociates the development of smooth muscle innervation from functional neurotransmission in mouse vas deferens." The Journal of physiology 581.2 (2007): 665-678. Mouse IHC 8 17379637
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Thompson, V. B., et al. "Prenatal exposure to MDMA alters noradrenergic neurodevelopment in the rat." Neurotoxicology and teratology 34.1 (2012): 206-213. Rat IHC 2 21978916
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Fang, Sheen-Yie, Ching-Liang Shen, and Masuru Ohyama. "Presence of neuropeptides in human nasal polyps." Acta oto-laryngologica 114.2 (1994): 324-328. Human 14 7521108
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Longley, C. D., and L. C. Weaver. "Proportions of renal and splenic postganglionic sympathetic populations containing galanin and dopamine beta hydroxylase." Neuroscience 55.1 (1993): 253-261. 8 7688881
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Clarke, Gwenaelle Laetitia. "Regulation of sympathetic plasticity in the heart." (2009). IHC 0 not found
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Flores, Juan A., et al. "Role for dopamine neurons of the rostral linear nucleus and periaqueductal gray in the rewarding and sensitizing properties of heroin." Neuropsychopharmacology 31.7 (2006): 1475-1488. Rat IHC, IF 26 16292327
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Takami, Toshihiro, et al. "Schwann cell but not olfactory ensheathing glia transplants improve hindlimb locomotor performance in the moderately contused adult rat thoracic spinal cord." The Journal of neuroscience 22.15 (2002): 6670-6681. Rat IHC 411 12151546
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Menei, Philippe, et al. "Schwann cells genetically modified to secrete human BDNF promote enhanced axonal regrowth across transected adult rat spinal cord." European Journal of Neuroscience 10.2 (1998): 607-621. Human, Rat IHC 296 9749723
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Kang, S. W., et al. "Serotonergic and Catecholaminergic Interactions with Co?Localised Dopamine?Melatonin Neurones in the Hypothalamus of the Female Turkey." Journal of neuroendocrinology 21.1 (2009): 10-19. Turkey ICC,IF 5 19094089
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Abrahamson, Eric E., and Robert Y. Moore. "Suprachiasmatic nucleus in the mouse: retinal innervation, intrinsic organization and efferent projections." Brain research 916.1 (2001): 172-191. Mouse 341 11597605
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Hasan W1, Jama A, Donohue T, Wernli G, Onyszchuk G, Al-Hafez B, Bilgen M, Smith PG, Brain Res. 2006 Dec 8;1124(1):142-54. Epub 2006 Nov 7. "Sympathetic hyperinnervation and inflammatory cell NGF synthesis following myocardial infarction in rats" Rat IF 17084822
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Garcia, C., et al. "The influence of specific noradrenergic and serotonergic lesions on the expression of hippocampal brain-derived neurotrophic factor transcripts following voluntary physical activity." Neuroscience 119.3 (2003): 721-732. 79 12809693
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Dev Neurobiol. 2008 Apr;68(5):656-68. doi: 10.1002/dneu.20611. The integration of song environment by catecholaminergic systems innervating the auditory telencephalon of adult female European starlings. Sockman KW1, Salvante KG. Bird IHC 18278799
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Walton, M. E., et al. "The mesocortical dopamine projection to anterior cingulate cortex plays no role in guiding effort-related decisions." Behavioral neuroscience 119.1 (2005): 323. Rat 37 15727537
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Sperlagh, B., et al. "The triangular septal nucleus as the major source of ATP release in the rat habenula: a combined neurochemical and morphological study." Neuroscience 86.4 (1998): 1195-1207. Rat 33 9697126
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Takada, M., T. Sugimoto, and T. Hattori. "Tyrosine hydroxylase immunoreactivity in cerebellar Purkinje cells of the rat." Neuroscience letters 150.1 (1993): 61-64. Rat 34 8097025
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Yagi, Hideshi, et al. "Vlgr1 knockout mice show audiogenic seizure susceptibility." Journal of neurochemistry 92.1 (2005): 191-202. Mouse 35 19539720
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Clarke GL1, Bhattacherjee A, Tague SE, Hasan W, Smith PG, J Neurosci. 2010 Sep 15;30(37):12446-54. doi: 10.1523/ JNEUROSCI. 1667-10.2010. "ß-adrenoceptor blockers increase cardiac sympathetic innervation by inhibiting autoreceptor suppression of axon growth" Rat IHC 15 20844139
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Tobari, Yasuko, et al. "A new pathway mediating social effects on the endocrine system: female presence acting via norepinephrine release stimulates gonadotropin-inhibitory hormone in the paraventricular nucleus and suppresses luteinizing hormone in quail." The Journal of Neuroscience 34.29 (2014): 9803-9811. Bird IF 11 not defined
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Bethea, Cynthia L., et al. "Hypothalamic KISS1 Expression, Gonadotrophin‐Releasing Hormone and Neurotransmitter Innervation Vary with Stress and Sensitivity in Macaques." Journal of neuroendocrinology 26.5 (2014): 267-281. Monkey 1 not defined
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Bethea, Cynthia L., et al. "Hypothalamic KISS1 Expression, Gonadotrophin‐Releasing Hormone and Neurotransmitter Innervation Vary with Stress and Sensitivity in Macaques." Journal of neuroendocrinology 26.5 (2014): 267-281. Monkey 1 not defined
22806 DBH (Dopamine-beta-Hydroxylase) Antibody Ikeda, Maaya Z., et al. "Norepinephrine Modulates Coding of Complex Vocalizations in the Songbird Auditory Cortex Independent of Local Neuroestrogen Synthesis." The Journal of Neuroscience 35.25 (2015): 9356-9368. Bird 0 not defined

Q & A Wall

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I am interested in using the dopamine-beta-hydroxylase(DBH) antibody in owl brain slice. This is ImmunoStar product number 22806. Has your DBH antibody been tested in any avian species?
Question By Kristin

No answer found for this question.

I am interested in using this to identify noradrenergic neurons in a rat using indirect immunofluorescence. Will this antibody be suitable? Also, does it matter if the antibody is polyclonal or monoclonal?
Question By Edmund T.

No answer found for this question.

Does this antibody react to all of the neuron of a rat brain, or only a particular part of the neuron? (e.g. axon, cell body)
Question By Er-Yun

The DBH antiserum stains in DBH. DBH containing neurons stain positively. I have also seen sporadic fiber staining. We typically QC the aby in rat brainstem

Answer By Jay on 2010-10-25 11:50:25

MSDS

This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.

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