CRF (Corticotropin Releasing Factor) Antibody

CRF (Corticotropin Releasing Factor) Antibody
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Price: $250.00
Product ID : 20084

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Description

The ImmunoStar antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat median eminence using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/1000 - 1/2000 in PBS/0.3% Triton X-100 - Biotin/Avidin-HRP Technique.

Immuno-labeling is completely abolished by preadsorption with synthetic CRF at 100 ug per mL of diluted antibody.

Photo Description: IHC image of the rat median eminence staining for CRF. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:2000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. The section was then mounted on slides with permount.   

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Reacts With: Mouse, Rat, Salmon, Turtle

Availability: In Stock

Alternate Names:  Corticoliberin; Corticotropin-Releasing Hormone,Endorpholiberin; corticotropin releasing hormone, anti-CRF

RRID:  AB_572228

Immunogen: Ovine CRF

Gene Symbol:  CRH

Entrez Gene ID: 101121525


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MSDS

This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.

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