CRF (Corticotropin Releasing Factor) Antibody

CRF (Corticotropin Releasing Factor) Antibody
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Price: $250.00
Product ID : 20084

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Description

The ImmunoStar antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat median eminence using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/1000 - 1/2000 in PBS/0.3% Triton X-100 - Biotin/Avidin-HRP Technique.

Immuno-labeling is completely abolished by preadsorption with synthetic CRF at 100 ug per mL of diluted antibody.

Photo Description: IHC image of the rat median eminence staining for CRF. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:2000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. The section was then mounted on slides with permount.   

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Reacts With: Mouse, Rat, Salmon, Turtle

Availability: In Stock

Alternate Names:  Corticoliberin; Corticotropin-Releasing Hormone,Endorpholiberin; corticotropin releasing hormone, anti-CRF

RRID:  AB_572228

Immunogen: Ovine CRF

Gene Symbol:  CRH

Entrez Gene ID: 101121525


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Publications

PROD # ANTIBODY CITATION SPECIES REACTIVITY APPL METHOD RESEARCH AREA # CITED BY PUBMED ID
20084 CRF (Corticotropin Releasing Factor) Antibody Larson, Brett A., et al. "A double sequential immunofluorescence method demonstrating the co-localization of urotensins I and II in the caudal neurosecretory system of the teleost, Gillichthys mirabilis." Cell and tissue research 247.2 (1987): 233-239. IHC 22 3545481
20084 CRF (Corticotropin Releasing Factor) Antibody McDonald, Alexander J., Franco Mascagni, and Marlene A. Wilson. "A sexually dimorphic population of CRF neurons in the medial preoptic area." Neuroreport 5.5 (1994): 653-656. 15 8025263
20084 CRF (Corticotropin Releasing Factor) Antibody Bean, ANDREW J., et al. "Acidic and Basic Fibroblast Growth Factors are Present in, and Produce Effects upon, Mesencephalic Dopamine Neurons." WENNER GREN INTERNATIONAL SERIES (1994): 267-267. Growth Factors 2 not found
20084 CRF (Corticotropin Releasing Factor) Antibody Reyes, Beverly AS, et al. "Agonist-induced internalization of corticotropin-releasing factor receptors in noradrenergic neurons of the rat locus coeruleus." European Journal of Neuroscience 23.11 (2006): 2991-2998. Rat IHC 44 16819988
20084 CRF (Corticotropin Releasing Factor) Antibody Huang, C. C., and E. H. Y. Lee. "Alteration of corticotropin?releasing factor immunoreactivity in MPTP?treated rats." Journal of neuroscience research 41.4 (1995): 471-480. Rat 8 7473878
20084 CRF (Corticotropin Releasing Factor) Antibody Onstott, Donna, and Robert Elde. "Coexistence of urotensin I/corticotropin-releasing factor and urotensin II immunoreactivities in cells of the caudal neurosecretory system of a teleost and an elasmobranch fish." General and comparative endocrinology 63.2 (1986): 295-300. IHC 17 3536660
20084 CRF (Corticotropin Releasing Factor) Antibody Zhang, Danru, and William D. Eldred. "Colocalization of enkephalin-, glucagon-, and corticotropin-releasing factor-like immunoreactivity in GABAergic amacrine cells in turtle retina." Brain research 596.1 (1992): 46-57. Turtle 17 1468002
20084 CRF (Corticotropin Releasing Factor) Antibody Ueyama, Teizo, et al. "Comparative distribution of GABAergic and peptide-containing neurons in the lateral lemniscal nuclei of the rat." Brain research 849.1 (1999): 220-225. Rat 5 10592305
20084 CRF (Corticotropin Releasing Factor) Antibody Kelley, Michael, and Neil Kowall. "Corticotropin-releasing factor immunoreactive neurons persist throughout the brain in Alzheimer's disease." Brain research 501.2 (1989): 392-396. alzheimer 26 2819447
20084 CRF (Corticotropin Releasing Factor) Antibody Cummings, S., et al. "Corticotropin-releasing factor immunoreactivity is widely distributed within the central nervous system of the rat: an immunohistochemical study." The Journal of Neuroscience 3.7 (1983): 1355-1368. 354 6345725
20084 CRF (Corticotropin Releasing Factor) Antibody Cummings, Sharon, Burt Sharp, and R. Elde. "Corticotropin-releasing factor in cerebellar afferent systems: a combined immunohistochemistry and retrograde transport study." The Journal of neuroscience 8.2 (1988): 543-554. IHC 56 3276832
20084 CRF (Corticotropin Releasing Factor) Antibody Ebbesson, L. O. E., et al. "Corticotropin-Releasing Factor Neurogenesis during Midlife Development in Salmon: Genetic, Environmental and Thyroid Hormone Regulation." Journal of neuroendocrinology 23.8 (2011): 733-741. Salmon IHC 11 21592238
20084 CRF (Corticotropin Releasing Factor) Antibody Preeyasombat, Chawalit, et al. "Cushing's syndrome caused by Ewing's sarcoma secreting corticotropin releasing factor-like peptide." American Journal of Diseases of Children 146.9 (1992): 1103-1105. 13 1325112
20084 CRF (Corticotropin Releasing Factor) Antibody Ikeda, M., et al. "Distribution and cerebellar projections of cholinergic and corticotropin-releasing factor-containing neurons in the caudal vestibular nuclear complex and adjacent brainstem structures." Neuroscience 49.3 (1992): 635-651. 22 1380135
20084 CRF (Corticotropin Releasing Factor) Antibody Chung, R. Y., et al. "Edinger-Westphal nucleus: cells that project to spinal cord contain corticotropin-releasing factor." Neuroscience letters 83.1 (1987): 13-19. IHC 20 not found
20084 CRF (Corticotropin Releasing Factor) Antibody Ye, X., et al. "Effect of infection with the 139H scrapie strain on the number, area and/or location of hypothalamic CRF-and VP-immunostained neurons." Acta neuropathologica 88.1 (1994): 44-54. 12 7941971
20084 CRF (Corticotropin Releasing Factor) Antibody Bachoo, Manjit, John Ciriello, and Canio Polosa. "Effect of preganglionic stimulation on neuropeptide-like immunoreactivity in the stellate ganglion of the cat." Brain research 400.2 (1987): 377-382. 33 3815084
20084 CRF (Corticotropin Releasing Factor) Antibody Onstott, Donna, and Robert Elde. "Immunohistochemical localization of urotensin I/corticotropin?releasing factor, urotensin II, and serotonin immunoreactivities in the caudal spinal cord of nonteleost fishes." Journal of Comparative Neurology 249.2 (1986): 205-225. IHC 46 3525617
20084 CRF (Corticotropin Releasing Factor) Antibody Sithigorngul, Paisarn, Antony OW Stretton, and Cynthia Cowden. "Neuropeptide diversity in Ascaris: an immunocytochemical study." Journal of Comparative Neurology 294.3 (1990): 362-376. 68 2341616
20084 CRF (Corticotropin Releasing Factor) Antibody Kaneko, Takeshi, Yoshifumi Nakaya, and Noboru Mizuno. "Paucity of glutaminase?immunoreactive nonpyramidal neurons in the rat cerebral cortex." Journal of Comparative Neurology 322.2 (1992): 181-190. Rat 40 1381731
20084 CRF (Corticotropin Releasing Factor) Antibody Gregg, Kathleen V., and Georgia A. Bishop. "Peptide localization in the mouse inferior olive." Journal of chemical neuroanatomy 12.3 (1997): 211-220. Mouse 4 9141652
20084 CRF (Corticotropin Releasing Factor) Antibody Moga, Margaret M., and Thackery S. Gray. "Peptidergic efferents from the intercalated nuclei of the amygdala to the parabrachial nucleus in the rat." Neuroscience letters 61.1 (1985): 13-18. 33 2867498
20084 CRF (Corticotropin Releasing Factor) Antibody Okuda, Hirotsugu, et al. "The presence of corticotropin-releasing factor-like immunoreactive synaptic vesicles in axon terminals with nicotinic acetylcholine receptor-like immunoreactivity in the median eminence of the rat." Neuroscience letters 161.2 (1993): 183-186. 39 8272263
20084 CRF (Corticotropin Releasing Factor) Antibody McGillis, Joseph P., Nicholas R. Hall, and Allan L. Goldstein. "Thymosin fraction 5 (TF5) stimulates secretion of adrenocorticotropic hormone (ACTH) from cultured rat pituitaries." Life sciences 42.22 (1988): 2259-2268. 21 2453772

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MSDS

This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.

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