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The antibody has a proven and strong Biotin-Streptadvidin/HRP staining at a 1/2000-1/4000 dilution in rat amygdala, and spinal cord.
The specificity of the antiserum was evaluated by soluble pre-adsorption with the peptides in question at a final concentration of 10-5M. CGRP immunolabeling was completely abolished by pre-adsorption with rat α -CGRP and partially eliminated by pre-adsorption with rat Ÿ -CGRP. Pre-adsorption with the following peptides resulted in no loss of immunostaining: rat amylin, rat adrenomedulin, calcitonin, neurotensin, somatostatin, substance P, leucine enkephalin, methionine enkephalin, VIP, CCK-8, vasopressin and neuropeptide Y.
Photo Description: IHC image of the dorsal horn of the rat spinal cord staining for CGRP (above) and low magnification image of a coronal spinal cord section staining for CGRP (below). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for frozen sectioning. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:4000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
Quantity / Volume: 100 µL
State: Lyophilized Whole Serum
Reacts With: Bullfrog, Cat, Dog, Hamster, Human, Mouse, Mudpuppy (Necturus Maculosus), Ovine (Sheep), Pig, Platypus, Ram (Sheep), Rat
Availability: In Stock
Alternate Names: anti-CGRP
Immunogen: rat alpha-CGRP
Gene Symbol: Calca
Entrez Gene ID: 24241
NCBI Gene Aliases: CAL6,CGRP,Cal1,Calc,RATCAL6,calcitoni
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Q & A Wall
Regarding the rabbit against rat CGRP antibody (Product No: 24112) I would like to know if it stains frozen human tissues, (like punch tissue) by the immunofluorescence technique and if so, the working dilution.
Question By Fatima
We tested with immunohistochemistry on rat tissue for the CGRP antibody, but we have quite a few references using human tissue.
One highly cited publication is Abdel-Rahman, T. A., et al. "Immunohistochemical, morphological and functional changes in the peripheral sudomotor neuro-effector system in elderly people." Journal of the autonomic nervous system 37.3 (1992): 187-197.
Answer By Colleen H on 2015-01-07 13:09:14
This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.Download MSDS