C-FOS Antibody

C-FOS Antibody
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Price: $375.00
Product ID : 26209

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Description

For induction of c-fos protein activity rats were injected with 1.0 ml of 1.5 M NaCl per 100 grams of body weight. Negative control rats were injected with the same volume of normal saline. The ImmunoStar c-fos antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat paraventricular nucleus and supraoptic nucleus using indirect immunofluorescent and biotin/avidin-HRP techniques. No labeling was seen in negative control rats. Recommended primary dilutions for these methods are 1/200-1/400 in PBS/0.3% Triton X-100 - FITC Technique and 1/4000-1/6000 in PBS/0.3% Triton X-100 - biotin/avidin-HRP Technique.

Injection of intraperitoneal hypertonic saline to provoke fos expression is most usually used to set ICC staining parameters.  It is critical that tissue is harvested 60-90 minutes post injection for this and any other stress that is used.  Inject 1 ml/100 g body weight 1.5 M NaCl intraperitoneally.  Harvest brains 90 minutes post injection.  Time is critical.

Specificity of the antiserum was demonstrated by blockage of staining in experimental rats by omission of c-fos antibody or by substitution of antibody pre-incubated with synthetic peptide or the conjugate. Immunoblot analysis of mediobasal hypothalamus showed a single band of approximately 55-60 kD.

Photo Description: IHC image of neurons staining for c-Fos in the rat supraoptic nucleus. The tissue was fixed with 4% formaldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:5000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. The section was then mounted on slides with permount.

Host: Rabbit

Quantity / Volume: 100 µL

State: Lyophilized Whole Serum

Reacts With: Mouse, Rat

Availability: In Stock

Alternate Names:  Proto-oncogene c-Fos; Cellular oncogene; G0/G1 switch regulatory protein 7; Proto-oncogene protein; G0S7; p55; AP-1; FBJ murine osteosarcoma viral oncogene homolog, anti-CFOS

Gene Symbol / ID, Accession #:  FOS, 2353

RRID:  AB_572267

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Publications

PROD # ANTIBODY CITATION SPECIES REACTIVITY APPL METHOD RESEARCH AREA # CITED BY PUBMED ID
26209 C-FOS Antibody Campbell, Thomas, et al. "Coping strategies in male and female rats exposed to multiple stressors." Physiology & behavior 78.3 (2003): 495-504. Rat IHC 59 12676287
26209 C-FOS Antibody Schwartz, M. D., A. A. Nunez, and L. Smale. "Differences in the suprachiasmatic nucleus and lower subparaventricular zone of diurnal and nocturnal rodents." Neuroscience 127.1 (2004): 13-23. Rat IHC 56 15219664
26209 C-FOS Antibody Bardi, Massimo, et al. "Effort-Based Reward (EBR) training enhances neurobiological efficiency in a problem-solving task: Insights for depression therapies." Brain research 1490 (2013): 101-110. 0 23085313
26209 C-FOS Antibody Franssen, C. L., et al. "Fatherhood alters behavioural and neural responsiveness in a spatial task." Journal of neuroendocrinology 23.11 (2011): 1177-1187. Mouse 6 21933288
26209 C-FOS Antibody Borbély S1, Halasy K, Somogyvári Z, Détári L, Világi I, Brain Res Bull. 2006 Mar 31;69(2):161-7. Epub 2005 Dec 19. "Laminar analysis of initiation and spread of epileptiform discharges in three in vitro models" 6 16533665
26209 C-FOS Antibody Owens, N. C., D. M. Sartor, and A. J. M. Verberne. "Medial prefrontal cortex depressor response: role of the solitary tract nucleus in the rat." Neuroscience 89.4 (1999): 1331-1346. Rat 39 10362318
26209 C-FOS Antibody Lambert, K. G., et al. "Modeling paternal attentiveness: distressed pups evoke differential neurobiological and behavioral responses in paternal and nonpaternal mice." Neuroscience 234 (2013): 1-12. Mouse 1 23262236
26209 C-FOS Antibody Hawley, Darby F., et al. "Neurobiological constituents of active, passive, and variable coping strategies in rats: integration of regional brain neuropeptide Y levels and cardiovascular responses." Stress: The International Journal on the Biology of Stress 13.2 (2009): 172-183. Rat 16 20214438
26209 C-FOS Antibody Thompson Haskell, Gloria, et al. "Retinoic acid signaling at sites of plasticity in the mature central nervous system." Journal of Comparative Neurology 452.3 (2002): 228-241. 57 12353219

Q & A Wall

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We are looking at CFOS activation following a behavior task in rats to look at level of activation in different brain regions between different conditions. Will your antibody only work if the rats are injected with hypertonic saline? That sounds like it will give us inaccurate activation information.
Question By Emily B

cFos expession can be induced by a variety of means: (drugs, ie receptor agonists [we have got responses to 5HT1a, 5HT2a, 5HT2c, 5HT6 and 5HT agonists; there are other neurotransmitter agonists reported that work as well]; hormones (eg a number of reports on icv injected angiotensin II, CCK), physiological stimuli (tactile stimuli, pain, light, sound, epileptic loci, hypertonic saline.

Each stimulus for cfos expression results in cell nuclei localization, but each also has anatomically distinct distribution in the brain. cfos can be seen as a common pathway of many different stimuli. Its role in the nucleus is to promote gene expression of probably 100s of things that an activated cell needs to have to respond.

Hypertonic saline is a useful stressor (hyperosmotic stress) that is an easy, convenient method to induce cfos in a preceise location, magnocellular paraventricular and supraoptic neurons. In fact so much has been done using this stimulus of cfos expression that its primary use now is to validate cfos immunohistochemistry. If this doesnt work, nothing will. Its proper application is time dependent, max response time is 60-90 minutes. So work out the immunohistochemistry parameters in hypertonic saline injected animals and apply those ICC conditions to what you are really interested in studying.


Answer By Dr. Mark Brownfield on 2015-11-02 14:17:09

I usually dissolve my nicotine in physiological saline and I have been getting c-Fos labeling before. Do you have literature suggesting that hypertonic saline is better?
Question By Ozra D.

Injection of intraperitoneal hypertonic saline to provoke fos expression is most usually used to set ICC staining parameters. It is critical that tissue is harvested 60-90 minutes post injection for this and any other stress that is used.

Inject 1 ml/100 g body weight 1.5 M NaCl intraperitoneally. Harvest brains 90 minutes post injection. Time is critical.



Answer By Mark B. on 2015-10-02 09:35:19

MSDS

This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.

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