Beta-Endorphin Antibody

Beta-Endorphin Antibody
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Price: $250.00
Product ID : 20063

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Description

The antibody produces a strong biotin-streptavidin/HRP staining at a 1/1000 - 1/2000 dilution in rat anterior pituitary.

Staining is completely eliminated by pretreatment of the diluted antibody with 10-6 M of Beta-Endorphin. Pre-adsorption of the diluted antibody with 10-6M of the following substances had no effect on Beta-Endorphin labeling: methionine enkephalin, leucine enkaphalin, dynorphin A, dynorphin B, gamma-endorphin, alpha-endorphin, ACTH and alpha-melanocyte stimulating hormone.

Photo Description: IHC images of beta-endorphin staining in the rat anterior pituitary with FITC-conjugated secondary antibody (above) and with standard biotin-streptavidin/HRP and DAB chromogen (below). The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for frozen sectioning. Sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:2000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. 

Endorphin pituitary #20063

Host: Rabbit

Quantity / Volume: 100 µL

State: Lyophilized

Availability: In Stock

Reacts With: Carp, Cat, Guinea Pig, Human, Mouse, Newt, Rat

Preabsorption Control: Available at Sigma Aldrich, Catalog # E1142

Alternate Names:  Endorfin-beta; Endorphin-beta; proopiomelanocortin, anti-Beta-Endorphin

RRID:  AB_572220

Immunogen: Beta-Endorphin

Gene Symbol / ID, Accession #:  POMC,5443

NCBI Gene Aliases: ACTH,BE,Pomc-1,Pomc1,alpha-MSH,alphaMSH,beta-MSH,gamma-MS


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Publications

PROD # ANTIBODY CITATION SPECIES REACTIVITY APPL METHOD RESEARCH AREA # CITED BY PUBMED ID
20063 Beta-Endorphin Antibody Clifton, V. L., et al. "Corticotropin-releasing hormone and proopiomelanocortin-derived peptides are present in human myometrium." Journal of Clinical Endocrinology & Metabolism 83.10 (1998): 3716-3721. Human IHC 57 9768689
20063 Beta-Endorphin Antibody Bhathena, Sam J., et al. "Decreased plasma enkephalins in copper deficiency in man." The American journal of clinical nutrition 43.1 (1986): 42-46. Human 20 2934971
20063 Beta-Endorphin Antibody J Cell Biol. 2011 Mar 7;192(5):767-80. doi: 10.1083/jcb.201010127. Distinct ATOH1 and Neurog3 requirements define tuft cells as a new secretory cell type in the intestinal epithelium. Gerbe F1, van Es JH, Makrini L, Brulin B, Mellitzer G, Robine S, Romagnolo B, Shroyer NF, Bourgaux JF, Pignodel C, Clevers H, Jay P. Human, Mouse 67 21383077
20063 Beta-Endorphin Antibody Basso, N., et al. "Effect of ceruletide on pituitary-hypothalamic peptides and on emotion in man." Peptides 2 (1981): 71-75. 24 6178096
20063 Beta-Endorphin Antibody Rama Sastry, B. V., et al. "Enkephalin-and substance P-like immunoreactivities of mammalian sperm and accessory sex glands." Biochemical pharmacology 31.21 (1982): 3519-3522. Human, Rat RADIO 43 6184060
20063 Beta-Endorphin Antibody Proc Natl Acad Sci U S A. 1981 Feb;78(2):1255-9. Enkephalin-like immunoreactivity of olivocochlear nerve fibers in cochlea of guinea pig and cat. Fex J, Altschuler RA. Cat, Guinea Pig IF 121 7015329
20063 Beta-Endorphin Antibody Borgquist, Amanda, et al. "Estradiol Negatively Modulates the Pleiotropic Actions of Orphanin FQ/Nociceptin at Proopiomelanocortin Synapses." Neuroendocrinology 98.1 (2013): 60-72. IHC 2 not found
20063 Beta-Endorphin Antibody Hinshaw, Lerner B., et al. "Evaluation of naloxone for therapy of Escherichia coli shock: Species differences." Archives of Surgery 119.12 (1984): 1410. 27 6391420
20063 Beta-Endorphin Antibody Chronwall, Bibie M., et al. "Glial somatostatin-14 expression in the rat pituitary intermediate lobe: a possible neurotrophic function during development?." International Journal of Developmental Neuroscience 18.7 (2000): 685-692. Rat 6 10978847
20063 Beta-Endorphin Antibody Borgquist, Amanda, et al. "Gonadal Steroids Differentially Modulate the Actions of Orphanin FQ/Nociceptin at A Physiologically Relevant Circuit Controlling Female Sexual Receptivity." Journal of neuroendocrinology 26.5 (2014): 329-340. Mouse IHC 0 24617903
20063 Beta-Endorphin Antibody Weizman, Ronit, et al. "Immunoreactive [beta]-Endorphin, Cortisol, and Growth Hormone Plasma Levels in Obsessive-Compulsive Disorder." Clinical neuropharmacology 13.4 (1990): 297-302. 27 2208181
20063 Beta-Endorphin Antibody CESANO, A. "L. PINESSI, I. RAINERO, T. DE GENNARO, A. VIOLANTE, D. CASSANO, L. MATERA,* AND." Annals of the New York Academy of Sciences 650 (1992): 351. 0 1318663
20063 Beta-Endorphin Antibody Vethamany-Globus, Swani, and Gregory Michener. "Localization of endorphin-like immunoreactivity in the nervous system of the adult newt, Notophthalmus viridescens." Journal of Experimental Zoology 262.2 (1992): 172-179. Newt IHC 3 1583460
20063 Beta-Endorphin Antibody WEIZMAN, RONIT, et al. "Low Plasma Immunoreactive ??Endorphin Levels in Autism." Journal of the American Academy of Child & Adolescent Psychiatry 27.4 (1988): 430-433. autism 36 not found
20063 Beta-Endorphin Antibody Roy III, Benjamin F. "Method for detecting antibodies against neuropeptides and drugs in human body fluid." U.S. Patent No. 4,868,107. 19 Sep. 1989. Human 1 not found
20063 Beta-Endorphin Antibody Valevski, A., et al. "Neuroendocrine response to trihexyphenidyl in depressed patients." Clinical neuropharmacology 15.1 (1992): 27-33. 0 1576596
20063 Beta-Endorphin Antibody Saad, Mohammed F., et al. "Occult Cushing's disease presenting with acute psychosis." The American journal of medicine 76.4 (1984): 759-766. IHC 14 6711582
20063 Beta-Endorphin Antibody Weizman, Abraham, et al. "Plasma Cortisol, Prolactin, Growth Hormone, and Immunoreactive [beta]-Endorphin Response to Fenfluramine Challenge in Depressed Patients." Clinical neuropharmacology 11.3 (1988): 250-256. 47 2969772
20063 Beta-Endorphin Antibody Perna, Gian Piero, et al. "Plasma -endorphin response to exercise in patients with congestive heart failure." CHEST Journal 111.1 (1997): 19-22. Human 10 8995987
20063 Beta-Endorphin Antibody Stagg, Nicola Jane. "Reversal of Neuropathic Pain with Exercise is Mediated by Endogenous Opioids." (2007). Rat 0 21386701
20063 Beta-Endorphin Antibody Gastroenterology. 2009 Aug;137(2):598-606, 606.e1-2. doi: 10.1053/j.gastro.2009.02.070. Epub 2009 Mar 9. Release of endogenous opioids from duodenal enteroendocrine cells requires Trpm5. Kokrashvili Z1, Rodriguez D, Yevshayeva V, Zhou H, Margolskee RF, Mosinger B. Mouse IF 19272386
20063 Beta-Endorphin Antibody Sakharkar, A. J., et al. "Reproduction Phase-Related Expression of Endorphin-Like Immunoreactivity in the Nucleus Lateralis Tuberis of the Female Indian Major Carp Cirrhinus mrigala: Correlation with the Luteinising Hormone Cells?Ovary Axis." Journal of neuroendocrinology 18.5 (2006): 319-329. Carp ICC 18 16629830
20063 Beta-Endorphin Antibody COHN, DAVID V., et al. "Selective Localization of the Parathyroid Secretory Protein-I/Adrenal Medulla Chromogranin A Protein Family in a Wide Variety of Endocrine Cells of the Rat*." Endocrinology 114.6 (1984): 1963-1974. Rat IF parathyroid 204 6233131
20063 Beta-Endorphin Antibody Hughes, Sharon, et al. "Upregulation of the pro-opiomelanocortin gene in motoneurones after acrylamide administration in mice." Neuroscience letters 357.3 (2004): 232-234. Mouse 3 15003292
20063 Beta-Endorphin Antibody Chuganji, Sayaka, et al. "Hyperalgesia in an immobilized rat hindlimb: Effect of treadmill exercise using non-immobilized limbs." Neuroscience letters 584 (2015): 66-70. Rat Pain 0 not defined

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MSDS

This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.

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