The antibody has a proven maximum biotin-streptavidin/HRP staining at a 1/1000 - 1/2000 dilution in rat raphe nuclei. Optimal dilution will vary depending upon fixation, labeling technique and/or detection system; therefore, a dilution series is recommended.
The specificity of the antiserum was evaluated using a model system of gelatin-indole plugs by a method similar to published procedures (Shipper and Tilders, 1983). Results showed that the 5-HTP antibody dose dependently stained 5-HTP but did not stain any concentration of 5-HT or 5-HIAA.
The antiserum was also tested by pre-adsorption with indole/paraformaldehyde/BSA conjugates. Staining was completely blocked by pre-adsorption with 5-HTP conjugate and unaffected by 5-HIAA or 5-HT conjugate.
Photo Description: IHC image of neurons staining for 5-HTP in the raphe nucleus of the rat brainstem. The tissue was fixed with 4% formaldehyde in phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:2000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
Quantity / Volume: 100 µL
State: Lyophilized Whole Serum
Reacts With: Rat, Sea Slug (Aplysia Californica, Tritonia Diomedea
Availability: In Stock
Alternate Names: anti-5-HTP
Gene Symbol: Avp
Entrez Gene ID: 24221
NCBI Gene Aliases: DI, Va
|PROD #||ANTIBODY||CITATION||SPECIES REACTIVITY||APPL METHOD||RESEARCH AREA||# CITED BY||PUBMED ID|
|24446||5-HTP (5-Hydroxytryptophan) Antibody||Fickbohm, David J., et al. "Localization and quantification of 5?hydroxytryptophan and serotonin in the central nervous systems of Tritonia and Aplysia." Journal of Comparative Neurology 437.1 (2001): 91-105.||Sea Slug (Aplysia Californica, Tritonia Diomedea)||IHC||31||11477599|
|24446||5-HTP (5-Hydroxytryptophan) Antibody||Lavezzi HN1, Parsley KP, Zahm DS.Brain Struct Funct. 2012 Jul;217(3):719-34. doi: 10.1007/s00429-011-0368-z. Epub 2011 Dec 18. "Mesopontine rostromedial tegmental nucleus neurons projecting to the dorsal raphe and pedunculopontine tegmental nucleus: psychostimulant-elicited Fos expression and collateralization"||Rat||IF||22179106|
|24446||5-HTP (5-Hydroxytryptophan) Antibody||Thalhammer, M., et al. "Sentinel lymph node biopsy and axillary dissection in breast cancer: results and initial experience of a changed technique with subareolar intradermal blue dye injection only." European Journal of Cancer 37 (2001): S180-S181.||IHC||cancer||1||not found|
|24446||5-HTP (5-Hydroxytryptophan) Antibody||Normandin, Joseph J., and Anne Z. Murphy. "Serotonergic lesions of the periaqueductal gray, a primary source of serotonin to the nucleus paragigantocellularis, facilitate sexual behavior in male rats." Pharmacology Biochemistry and Behavior 98.3 (2011): 369-375.||Rat||IHC||6||21296106|
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This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.Download MSDS