5-HT (Serotonin) Transporter Antibody

5-HT (Serotonin) Transporter Antibody
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Price: $400.00
Product ID : 24330

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Description

The ImmunoStar serotonin (5HT) transporter was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat raphe nuclei, hypothalamus, cortex and spinal cord using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/10,000 - 1/15,000 in PBS/0.3% Triton X-100 - Biotin/avidin-HRP.

By Western blot analysis using rat brain extracts of cortex, hypothalamus, midbrain, and hindbrain, the antibody specifically labels a single band. The predicted molecular weight of serotonin transporter based on its nucleic acid sequence is 67,815.  The actual weight that one might observe from tissue extracts could be higher due to glycosylation.  We validated the antibody with rat SERT overexpressed in an insect cell expression system.  The MW from that was about 67 kD.  Our SERT antibody dilution was 1:20,000 using chemiluminescent detection.

Immunolabeling is completely abolished by pre-adsorption with synthetic rat 5HT transporter (602-622).

Photo Description: IHC image of neurons staining for the 5-HT transporter in the raphe nucleus of the rat brainstem (above). The tissue was fixed with 4% formaldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:10000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen. Florescent secondary (below)

5Ht Transporter #14330

Host: Rabbit

Quantity / Volume:  100 µL

State: Lyophilized Whole Serum

Availability: In Stock

Reacts With: Human, Marine, Monkey, Mouse, Rat, Sparrow

Alternate Names:  5HTT; HTT; Sodium-dependent serotonin transporter; Solute carrier family 6 member 4; Slc6a4; solute carrier family 6 (neurotransmitter transporter, serotonin), member 4

RRID:  AB_572209

Immunogen: Rat 5-HT transporter (579-599)

Gene Symbol / ID, Accession #:  Slc6a4,25553

Reviews

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Publications

PROD # ANTIBODY CITATION SPECIES REACTIVITY APPL METHOD RESEARCH AREA # CITED BY PUBMED ID
24330 5-HT (Serotonin) Transporter Antibody Exp Neurol. 2010 Jan;221(1):68-78. doi: 10.1016/j.expneurol.2009.10.003. Epub 2009 Oct 17. 5-HT precursor loading, but not 5-HT receptor agonists, increases motor function after spinal cord contusion in adult rats. Hayashi Y1, Jacob-Vadakot S, Dugan EA, McBride S, Olexa R, Simansky K, Murray M, Shumsky JS. Rat IHC 19840787
24330 5-HT (Serotonin) Transporter Antibody Bonnin, Alexandre, et al. "A transient placental source of serotonin for the fetal forebrain." Nature 472.7343 (2011): 347-350. Human, Mouse 103 21512572
24330 5-HT (Serotonin) Transporter Antibody Bertrand, R. L., et al. "A Western diet increases serotonin availability in rat small intestine." Endocrinology 152.1 (2011): 36-47. Rat WB 16 21068163
24330 5-HT (Serotonin) Transporter Antibody Kalous, Adrianna, Peregrine B. Osborne, and Janet R. Keast. "Acute and chronic changes in dorsal horn innervation by primary afferents and descending supraspinal pathways after spinal cord injury." Journal of Comparative Neurology 504.3 (2007): 238-253. Rat IHC 22 17640046
24330 5-HT (Serotonin) Transporter Antibody Rumajogee, Prakasham, et al. "Adaption of the serotoninergic neuronal phenotype in the absence of 5?HT autoreceptors or the 5?HT transporter: involvement of BDNF and cAMP." European Journal of Neuroscience 19.4 (2004): 937-944. Mouse IHC 34 15009141
24330 5-HT (Serotonin) Transporter Antibody Arrant, Andrew E., Hikma Jemal, and Cynthia M. Kuhn. "Adolescent male rats are less sensitive than adults to the anxiogenic and serotonin-releasing effects of fenfluramine." Neuropharmacology 65 (2013): 213-222. Rat IF 6 23103347
24330 5-HT (Serotonin) Transporter Antibody J Comp Neurol. 2006 Nov 1;499(1):64-89. Afferent and efferent connections of the rat retrotrapezoid nucleus. Rosin DL1, Chang DA, Guyenet PG. Rat IF 16958085
24330 5-HT (Serotonin) Transporter Antibody Bruzos-Cidón C1, Miguelez C2, Rodríguez JJ3, Gutiérrez-Lanza R4, Ugedo L1, Torrecilla M5, Eur Neuropsychopharmacol. 2014 ul;24(7):1112-22. doi: 10.1016/j.euroneuro.2014.02.007. Epub 2014 Feb 19. "Altered neuronal activity and differential sensitivity to acute antidepressants of locus coeruleus and dorsal raphe nucleus in Wistar Kyoto rats: a comparative study with Sprague Dawley and Wistar rats" Mouse IHC 0 24582527
24330 5-HT (Serotonin) Transporter Antibody J Comp Neurol. 2008 Sep 20;510(3):283-96. doi: 10.1002/cne.21785. Anatomical and functional characterization of neuropil in the gracile fasciculus. Ramer MS1. Rat IHC 18634004
24330 5-HT (Serotonin) Transporter Antibody Van Dine, Sarah E., et al. "Axonal anatomy of molecular layer heterotopia of the cerebellar vermis." Journal of chemical neuroanatomy 47 (2013): 90-95. 1 23261868
24330 5-HT (Serotonin) Transporter Antibody J Neurosci. 2006 Apr 19;26(16):4338-49. Barrel map development relies on protein kinase A regulatory subunit II beta-mediated cAMP signaling. Inan M1, Lu HC, Albright MJ, She WC, Crair MC. Mouse IF 16624954
24330 5-HT (Serotonin) Transporter Antibody Mamounas, Laura A., et al. "BDNF promotes the regenerative sprouting, but not survival, of injured serotonergic axons in the adult rat brain." The Journal of Neuroscience 20.2 (2000): 771-782. Rat ICC 265 10632606
24330 5-HT (Serotonin) Transporter Antibody Seimandi, Mathieu, et al. "Calcineurin Interacts with the Serotonin Transporter C-Terminus to Modulate Its Plasma Membrane Expression and Serotonin Uptake." The Journal of Neuroscience 33.41 (2013): 16189-16199. Mouse 0 24107951
24330 5-HT (Serotonin) Transporter Antibody Van Dine, Sarah E., et al. "Cellular and Axonal Diversity in Molecular Layer Heterotopia of the Rat Cerebellar Vermis." BioMed research international 2013 (2013). Rat 0 24191251
24330 5-HT (Serotonin) Transporter Antibody Dudok, Jacobus J., et al. "Chronic activation of the 5-HT 2 receptor reduces 5-HT neurite density as studied in organotypic slice cultures." Brain research 1302 (2009): 1-9. 5 19728996
24330 5-HT (Serotonin) Transporter Antibody Dudok, Jacobus J., et al. "Chronic activation of the 5-HT< 2< receptor reduces 5-HT neurite density as studied in organotypic slice cultures." Brain research 1302 (2009): 1-9. 5 19728996
24330 5-HT (Serotonin) Transporter Antibody Sanno, Hitomi, et al. "Control of Postnatal Apoptosis in the Neocortex byRhoA-Subfamily GTPases Determines Neuronal Density." The Journal of Neuroscience 30.12 (2010): 4221-4231. Mouse Apoptosis 13 20335457
24330 5-HT (Serotonin) Transporter Antibody Iwasato, Takuji, et al. "Cortex-restricted disruption of NMDAR1 impairs neuronal patterns in the barrel cortex." Nature 406.6797 (2000): 726-731. Mouse IHC 308 10963597
24330 5-HT (Serotonin) Transporter Antibody Nayyar, Tultul, et al. "Cortical serotonin and norepinephrine denervation in parkinsonism: preferential loss of the beaded serotonin innervation." European Journal of Neuroscience 30.2 (2009): 207-216. Mouse IHC parkinson 19 19659923
24330 5-HT (Serotonin) Transporter Antibody Cragg, J. J., A. L. Scott, and M. S. Ramer. "Depletion of spinal 5-HT accelerates mechanosensory recovery in the deafferented rat spinal cord." Experimental neurology 222.2 (2010): 277-284. Rat 5 20079735
24330 5-HT (Serotonin) Transporter Antibody Scott, Angela LM, and Matt S. Ramer. "Differential regulation of dendritic plasticity by neurotrophins following deafferentation of the adult spinal cord is independent of p75< sup< NTR 3 20144886
24330 5-HT (Serotonin) Transporter Antibody Brown, Pierre, and Mark E. Molliver. "Dual serotonin (5-HT) projections to the nucleus accumbens core and shell: relation of the 5-HT transporter to amphetamine-induced neurotoxicity." The Journal of Neuroscience 20.5 (2000): 1952-1963. Rat IHC 111 10684896
24330 5-HT (Serotonin) Transporter Antibody Esmaili, Ali, et al. "Enteropathogenic< i< Escherichia coli Infection Inhibits Intestinal Serotonin Transporter Function and Expression." Gastroenterology 137.6 (2009): 2074-2083. Mouse WB 15 19747920
24330 5-HT (Serotonin) Transporter Antibody Matragrano LL1, Sanford SE, Salvante KG, Beaulieu M, Sockman KW, Maney DL., Behav Neurosci. 2012 Feb;126(1):110-22. doi: 10.1037/a0025586. Epub 2011 Sep 26. "Estradiol-dependent modulation of serotonergic markers in auditory areas of a seasonally breeding songbird" Sparrow IF 21942431
24330 5-HT (Serotonin) Transporter Antibody J Neurochem. 2006 Jan;96(2):396-406. Epub 2005 Nov 21. Evidence that serotonin reuptake modulators increase the density of serotonin innervation in the forebrain. Zhou L1, Huang KX, Kecojevic A, Welsh AM, Koliatsos VE. Rat IHC 16300628
24330 5-HT (Serotonin) Transporter Antibody Thompson AM1, Thompson GC, Brain Res. 2009 Feb 9;1253:60-8. doi: 0.1016/j.brainres.2008. 11.054. Epub 2008 Nov 28. "Experimental evidence that the serotonin transporter mediates serotonin accumulation in LSO neurons of the postnatal mouse". . Mouse IF 19070605
24330 5-HT (Serotonin) Transporter Antibody Lee, Li?Jen, et al. "Exuberant thalamocortical axon arborization in cortex?specific NMDAR1 knockout mice." Journal of Comparative Neurology 485.4 (2005): 280-292. Mouse 63 15803506
24330 5-HT (Serotonin) Transporter Antibody Assimacopoulos, Stavroula, et al. "Fibroblast growth factor 8 organizes the neocortical area map and regulates sensory map topography." The Journal of Neuroscience 32.21 (2012): 7191-7201. Mouse IHC 10 22623663
24330 5-HT (Serotonin) Transporter Antibody Shimogori, Tomomi, and Elizabeth A. Grove. "Fibroblast growth factor 8 regulates neocortical guidance of area-specific thalamic innervation." The Journal of neuroscience 25.28 (2005): 6550-6560. Mouse 61 16014716
24330 5-HT (Serotonin) Transporter Antibody Gill RK1, Pant N, Saksena S, Singla A, Nazir TM, Vohwinkel L, Turner JR, Goldstein J, Alrefai WA, Dudeja PK., Am J Physiol Gastrointest Liver Physiol. 2008 Jan;294(1):G254-62. Epub 2007 Nov 8."Function, expression, and characterization of the serotonin transporter in the native human intestine" Human WB, IF 17991706
24330 5-HT (Serotonin) Transporter Antibody Donovan, Stacy L., et al. "GAP-43 is critical for normal development of the serotonergic innervation in forebrain." The Journal of neuroscience 22.9 (2002): 3543-3552. Mouse 82 11978831
24330 5-HT (Serotonin) Transporter Antibody Li, Kairong, Jiaqing Zhang, and James YH Li. "Gbx2 plays an essential but transient role in the formation of thalamic nuclei." PloS one 7.10 (2012): e47111. Mouse IF,IHC 2 23056596
24330 5-HT (Serotonin) Transporter Antibody Neuroscience. 1996 Jul;73(1):217-31. Immunocytochemical detection of the serotonin transporter in rat brain. Sur C1, Betz H, Schloss P. Rat IF 8783244
24330 5-HT (Serotonin) Transporter Antibody Hong, Diana. "Immunohistochemical quantification of serotonin and serotonin transporters in the lumbar region of the mouse spinal cord after spinal cord injury." (2013). Rat 0 not found
24330 5-HT (Serotonin) Transporter Antibody Noristani HN1, Meadows RS, Olabarria M, Verkhratsky A, Rodríguez JJ, Cell Death Dis. 2011 Sep 15;2:e210. doi: 10.1038/cddis.2011.79. "Increased hippocampal CA1 density of serotonergic terminals in a triple transgenic mouse model of Alzheimer's disease: an ultrastructural study" Mouse IF alzheimer 21918544
24330 5-HT (Serotonin) Transporter Antibody J Neurophysiol. 2007 Sep;98(3):1610-25. Epub 2007 Jun 20. Increased thalamocortical synaptic response and decreased layer IV innervation in GAP-43 knockout mice. Albright MJ1, Weston MC, Inan M, Rosenmund C, Crair MC. Mouse IF 17581849
24330 5-HT (Serotonin) Transporter Antibody J Neurotrauma. 2011 Mar;28(3):479-92. doi: 10.1089/neu.2010.1609. Intermittent fasting improves functional recovery after rat thoracic contusion spinal cord injury. Jeong MA1, Plunet W, Streijger F, Lee JH, Plemel JR, Park S, Lam CK, Liu J, Tetzlaff W. Rat IF 21219083
24330 5-HT (Serotonin) Transporter Antibody Koldzic-Zivanovic, Nina, et al. "Intracellular signaling involved in estrogen regulation of serotonin reuptake." Molecular and cellular endocrinology 226.1 (2004): 33-42. Rat IHC 52 15489003
24330 5-HT (Serotonin) Transporter Antibody Peters, Christopher M., et al. "Lack of analgesic efficacy of spinal ondansetron on thermal and mechanical hypersensitivity following spinal nerve ligation in the rat." Brain research 1352 (2010): 83-93. Rat IHC 14 20637741
24330 5-HT (Serotonin) Transporter Antibody Datwani, Akash, et al. "Lesion-induced thalamocortical axonal plasticity in the S1 cortex is independent of NMDA receptor function in excitatory cortical neurons." The Journal of neuroscience 22.21 (2002): 9171-9175. Mouse IHC 28 12417641
24330 5-HT (Serotonin) Transporter Antibody Sur, Cyrille, Heinrich Betz, and Patrick Schloss. "Localization of the serotonin transporter in rat spinal cord." European Journal of Neuroscience 8.12 (1996): 2753-2757. Rat 34 8996825
24330 5-HT (Serotonin) Transporter Antibody Xie, Tao, et al. "Loss of serotonin transporter protein after MDMA and other ring-substituted amphetamines." Neuropsychopharmacology 31.12 (2006): 2639-2651. Rat 56 16452989
24330 5-HT (Serotonin) Transporter Antibody Aristieta, A., et al. "Modulation of the subthalamic nucleus activity by serotonergic agents and fluoxetine administration." Psychopharmacology (2013): 1-12. Rat IHC 2 24271033
24330 5-HT (Serotonin) Transporter Antibody Zhang, Junlin, et al. "Neonatal citalopram exposure decreases serotonergic fiber density in the olfactory bulb of male but not female adult rats." Frontiers in cellular neuroscience 7 (2013). Rat IHC 3 23675318
24330 5-HT (Serotonin) Transporter Antibody Weaver, Kristin J., et al. "Neonatal exposure to citalopram selectively alters the expression of the serotonin transporter in the hippocampus: dose?dependent effects." The Anatomical Record 293.11 (2010): 1920-1932. Rat IHC 20 20830689
24330 5-HT (Serotonin) Transporter Antibody Meyer, Jerrold S., et al. "Neurotoxic effects of MDMA ( Rat 50 15380826
24330 5-HT (Serotonin) Transporter Antibody J Neurochem. 2006 Nov;99(3):1019-31. Epub 2006 Sep 18. Ontogeny of brain and blood serotonin levels in 5-HT receptor knockout mice: potential relevance to the neurobiology of autism. Janusonis S1, Anderson GM, Shifrovich I, Rakic P. Mouse Neurobiology, Autism 34 16981893
24330 5-HT (Serotonin) Transporter Antibody Linley, Stephanie B., Walter B. Hoover, and Robert P. Vertes. "Pattern of distribution of serotonergic fibers to the orbitomedial and insular cortex in the rat." Journal of chemical neuroanatomy 48 (2013): 29-45. Rat 1 23337940
24330 5-HT (Serotonin) Transporter Antibody Papesh, Melissa A., and Laura M. Hurley. "Plasticity of serotonergic innervation of the inferior colliculus in mice following acoustic trauma." Hearing research 283.1 (2012): 89-97. Mouse IHC 4 22101024
24330 5-HT (Serotonin) Transporter Antibody De Silva, D. J., et al. "Rat brain serotonin neurones that express neuronal nitric oxide synthase have increased sensitivity to the substituted amphetamine serotonin toxins 3, 4-methylenedioxymethamphetamine andp-chloroamphetamine." Neuroscience 134.4 (2005): 1363-1375. Rat 10 16054768
24330 5-HT (Serotonin) Transporter Antibody Kubota, Nobuo, et al. "Regulation of serotonin transporter gene expression in human glial cells by growth factors." European journal of pharmacology 417.1 (2001): 69-76. Human Growth Factors 28 11301061
24330 5-HT (Serotonin) Transporter Antibody RSynapse. 2011 Sep;65(9):919-28. doi: 10.1002/syn.20920. Epub 2011 Mar 28. Serotonergic projections and serotonin receptor expression in the reticular nucleus of the thalamus in the rat. Rodríguez JJ1, Noristani HN, Hoover WB, Linley SB, Vertes RP. Rat IHC 6 21308802
24330 5-HT (Serotonin) Transporter Antibody Hayat, Shaista, Robert J. Williams, and Marcus Rattray. "Serotonin transporter expression is not sufficient to confer cytotoxicity to 3, 4-methylenedioxymethamphetamine (MDMA) in vitro." Journal of Psychopharmacology 20.2 (2006): 257-263. Mouse, Rat WB 10 16510483
24330 5-HT (Serotonin) Transporter Antibody J Neurosci. 2007 Sep 5;27(36):9545-59. Skin-derived precursors generate myelinating Schwann cells that promote remyelination and functional recovery after contusion spinal cord injury. Biernaskie J1, Sparling JS, Liu J, Shannon CP, Plemel JR, Xie Y, Miller FD, Tetzlaff W. Rat IF 17804616
24330 5-HT (Serotonin) Transporter Antibody Kalous, Adrianna, Peregrine B. Osborne, and Janet R. Keast. "Spinal cord compression injury in adult rats initiates changes in dorsal horn remodeling that may correlate with development of neuropathic pain." Journal of Comparative Neurology 513.6 (2009): 668-684. Rat 13 19235905
24330 5-HT (Serotonin) Transporter Antibody Husch, Andreas, et al. "Spinal cord injury induces serotonin supersensitivity without increasing intrinsic excitability of mouse V2a interneurons." The Journal of Neuroscience 32.38 (2012): 13145-13154. Mouse IHC 5 22993431
24330 5-HT (Serotonin) Transporter Antibody Neurochem Res.2005 Jun-Jul;30(6-7): 825-37.The pre-nervous serotonergic system of developing sea urchin embryos and larvae: pharmacologic and immunocytochemical evidence.Buznikov GA1, Peterson RE, Nikitina LA, Bezuglov VV, Lauder JM. Marine IHC 16187217
24330 5-HT (Serotonin) Transporter Antibody J Neurosci. 2011 Apr 13;31(15):5605-16. doi: 10.1523/JNEUROSCI.6663-10.2011. The unusual response of serotonergic neurons after CNS Injury: lack of axonal dieback and enhanced sprouting within the inhibitory environment of the glial scar. Hawthorne AL1, Hu H, Kundu B, Steinmetz MP, Wylie CJ, Deneris ES, Silver J. Rat IF 21490201
24330 5-HT (Serotonin) Transporter Antibody André P1, Saubaméa B, Cochois-Guégan V, Marie-Claire C, Cattelotte J, Smirnova M, Schinkel AH, Scherrmann JM, Cisternino S., J Cereb Blood Flow Metab. 2012 Nov;32(11):1989-2001. doi: 10.1038/jcbfm.2012.109. Epub 2012 Aug 1."Transport of biogenic amine neurotransmitters at the mouse blood-retina and blood-brain barriers by uptake1 and uptake2" Mouse IHC 3 22850405
24330 5-HT (Serotonin) Transporter Antibody Bretzner, Frederic, et al. "Undesired effects of a combinatorial treatment for spinal cord injury Mouse 47 18973595
24330 5-HT (Serotonin) Transporter Antibody Rumajogee, Prakasham, et al. "Up?regulation of the neuronal serotoninergic phenotype in vitro: BDNF and cAMP share Trk B?dependent mechanisms." Journal of neurochemistry 83.6 (2002): 1525-1528. Rat IHC 62 12472905
24330 5-HT (Serotonin) Transporter Antibody Emiliano, Ana BF, et al. "The interface of oxytocin-labeled cells and serotonin transporter-containing fibers in the primate hypothalamus: a substrate for SSRIs therapeutic effects?" Neuropsychopharmacology 32.5 (2007): 977-988. Monkey (Macaque) IF 44 17035935
24330 5-HT (Serotonin) Transporter Antibody Alter, Shawn P., et al. "Reduced vesicular monoamine transport disrupts serotonin signaling but does not cause serotonergic degeneration." Experimental neurology (2015). Mouse 0 not defined
24330 5-HT (Serotonin) Transporter Antibody Smith, Victoria M., Ryan T. Jeffers, and Michael C. Antle. "Serotonergic enhancement of circadian responses to light: role of the raphe and intergeniculate leaflet." European Journal of Neuroscience (2015). Hamster hamster hamster 0 not defined
24330 5-HT (Serotonin) Transporter Antibody Keber, U., et al. "Striatal tyrosine hydroxylase-positive neurons are associated with l-DOPA-induced dyskinesia in hemiparkinsonian mice." Neuroscience 298 (2015): 302-317. Mouse Parkinson's 0 not defined

Q & A Wall

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First, is the antibody specific to SERT, or is there affinity for DAT or NET? Also, can the antibody be used for mouse and human as well as for rat? Has this antibody been used for immunoprecipitation?
Question By Michael W

The antibody is specific for SerT and does not cross react with DAT or NET.

The antiserum was characterized anatomically by Western blot and by preadsorption with a SerT specific peptide. A BLAST search of the peptide sequence finds only SerT from different species. The sequence is 100% homologous with rat (because that's what was chosen to raise it against).

It is 90% homologous with mouse so it will probably work. It is 85% homologous with human so it might work, but we haven't tried it.

The antibody has not been used for immunoprecipitation; not that it wouldn't work, but we've not tried it. We have used Vector ABC kits with nice results and secondary fluorescent antisera such as Cy3, also with nice results. Thinner sections work best since there are numerous fine fibers in many areas of the CNS.




Answer By Tech Support on 2006-09-22 13:44:20

The product specification sheet suggests a single band reactivity of SERT to multiple regions of rat brain on western blots. I am wondering if you could perhaps please share the western blot images showing the performance of this antibody to brain extracts of rat as mentioned in the spec sheet. We were unable to find any western blot images in the references listed on the website.
Question By Chandana

I looked through our library of references but unfortunately could not find an article in which rat tissue was used with western blot using the 5-HT Transporter antibody. However I have information from our lab from a few years ago:

The predicted molecular weight of serotonin transporter based on its nucleic acid sequence is 67,815. The actual weight that one might observe from tissue extracts could be higher due to glycosylation. We validated the antibody with rat SERT overexpressed in an insect cell expression system. The MW from that was about 67 kD. Our SERT antibody dilution was 1:20,000 using chemiluminescent detection.



Answer By Colleen on 0000-00-00 00:00:00

MSDS

This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.

Download MSDS