5-HT (Serotonin) 7 Receptor Antibody

5-HT (Serotonin) 7 Receptor Antibody
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Price: $375.00
Product ID : 24430

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Description

The ImmunoStar 5-HT7 receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat cortex and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/100 - 1/300 in PBS - biotin/avidin-HRP Technique. Note: use of Triton X-100 or other detergents is not recommended.

The antibody was characterized by immunohistochemistry. Immunohistochemical staining of rat brain correlates well with Northern blot analysis, in situ hybridization and receptor autoradiography studies. Immunolabeling is completely abolished by preadsorption with synthetic rat 5-HT7 receptor (8-23). BlastP database sequence homology searches indicate that the amino acid sequence is unique to rat 5-HT7A, 5-HT7B and 5-HT7C. There is also significant sequence overlap with the mouse and human forms.

Photo Description: IHC image of neurons staining for the 5-HT7 receptor in the rat brain cortex. The tissue was fixed with 4% formaldehyde/0.05% glutaraldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:500 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

Host: Rabbit

Quantity/Volume: 100 µL

State: Liquid

Reacts With: Cat, Grasshopper, Human, Hutchinsoniella Macracantha (Horseshoe Shrimp), Mouse, Pig, Rabbit, Rat

Availability:  In stock

Alternate Names:  5-hydroxytryptamine receptor 7; 5-HT-X,GPRFO; 5Ht7; 5-hydroxytryptamine (serotonin) receptor 7, anti-5-HT 7

RRID: AB_572214

Immunogen: Rat 5-HT7 receptor (8-23)

Gene Symbol / ID, Accession #:  Htr7,65032


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Publications

PROD # ANTIBODY CITATION SPECIES REACTIVITY APPL METHOD RESEARCH AREA # CITED BY PUBMED ID
24430 5-HT (Serotonin) 7 Receptor Antibody Muneoka, Katsumasa T., and Morikuni Takigawa. "5-Hydroxytryptamine 7(5-HT 7) receptor immunoreactivity-positive Rat 35 12711351
24430 5-HT (Serotonin) 7 Receptor Antibody Itoh, Takeo, and Junko Kajikuri. "Characteristics of the actions by which 5?HT affects electrical and mechanical activities in rabbit jugular vein." British journal of pharmacology 164.3 (2011): 979-991. Rabbit IF 2 21449974
24430 5-HT (Serotonin) 7 Receptor Antibody Maekawa, Takashi, et al. "Characteristics of the actions by which 5?hydroxytryptamine affects electrical and mechanical activities in rabbit jugular vein graft." British journal of pharmacology 166.4 (2012): 1419-1432. Rabbit IF 4 22251164
24430 5-HT (Serotonin) 7 Receptor Antibody Turner, Helen C., et al. "Characterization of serotonergic receptors in rabbit, porcine and human conjunctivae." Current eye research 27.4 (2003): 205-215. Human, Pig, Rabbit WB, IF 10 14562171
24430 5-HT (Serotonin) 7 Receptor Antibody Iwasato, Takuji, et al. "Cortical adenylyl cyclase 1 is required for thalamocortical synapse maturation and aspects of layer IV barrel development." The Journal of Neuroscience 28.23 (2008): 5931-5943. Mouse IF 26 18524897
24430 5-HT (Serotonin) 7 Receptor Antibody Khalmuratova, Roza, et al. "Immunohistochemical and biomolecular identification of 5-HT _ {7} receptor in rat vestibular nuclei." Journal of Vestibular Research 20.6 (2010): 401-406. Rat IHC 3 21248402
24430 5-HT (Serotonin) 7 Receptor Antibody Noga, Brian R., et al. "Locomotor-activated neurons of the cat. I. Serotonergic innervation and co-localization of 5-HT7, 5-HT2A, and 5-HT1A receptors in the thoraco-lumbar spinal cord." Journal of neurophysiology 102.3 (2009): 1560. Cat ICC 19 19571190
24430 5-HT (Serotonin) 7 Receptor Antibody Brenchat, Alex, et al. "Pharmacological activation of 5-HT 7 receptors reduces nerve injury-induced mechanical and thermal hypersensitivity." Pain 149.3 (2010): 483-494. Mouse IHC 23 20399562
24430 5-HT (Serotonin) 7 Receptor Antibody Contesse, Vincent, et al. "Pharmacological and molecular characterization of 5-hydroxytryptamine7 receptors in the rat adrenal gland." Molecular pharmacology 56.3 (1999): 552-561. Rat WB 31 10462543
24430 5-HT (Serotonin) 7 Receptor Antibody J Comp Neurol. 2005 Sep 26;490(3):256-69. Pre- and postsynaptic localization of the 5-HT7 receptor in rat dorsal spinal cord: immunocytochemical evidence. Doly S1, Fischer J, Brisorgueil MJ, Vergé D, Conrath M. Rat WB 80 16082681
24430 5-HT (Serotonin) 7 Receptor Antibody Pain. 2005 Sep;117(1-2):182-92. Pronociceptive role of peripheral and spinal 5-HT7 receptors in the formalin test.Rocha-González HI1, Meneses A, Carlton SM, Granados-Soto V. Rat IHC 65 16098671
24430 5-HT (Serotonin) 7 Receptor Antibody Condron, Barry G., and Kai Zinn. "Regulated neurite tension as a mechanism for determination of neuronal arbor geometries in vivo." Current Biology 7.10 (1997): 813-816. Grasshopper 27 9368767
24430 5-HT (Serotonin) 7 Receptor Antibody Parga, J., et al. "Serotonin decreases generation of dopaminergic neurons from mesencephalic precursors via serotonin type 7 and type 4 receptors." Developmental neurobiology 67.1 (2007): 10-22. Rat IHC 10 17443768
24430 5-HT (Serotonin) 7 Receptor Antibody Belenky, Michael A., and Gary E. Pickard. "Subcellular distribution of 5-HT1b and 5-HT7 receptors in the mouse suprachiasmatic nucleus." Journal of Comparative Neurology 432.3 (2001): 371-388. Mouse IHC 87 11246214

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MSDS

This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.

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