5-HT (Serotonin) 5A Receptor Antibody

5-HT (Serotonin) 5A Receptor Antibody
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Price: $375.00
Product ID : 24429

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Description

The ImmunoStar 5-HT5A Receptor was quality control tested using standard immunohisto-chemical methods. The antiserum demonstrates strongly positive labeling of rat cortex and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilution is 1/200 - 1/400 in PBS/0.3% Triton X-100 - Bn/Av-HRP. Intensification methods such as nickel will approximately double the dilution factor as recommended.

The antibody was characterized by immunoblotting and immunohistochemistry. Immunoblots of rat brain extracts revealed the presence of two bands at molecular weights of 41 and 47 kD. The lower weight band agrees with the calculated molecular weight based on amino acid sequence. The higher weight may represent glycosylated receptor protein.

Immunohistochemical staining of rat brain correlates well with Northern blot analysis and in situ hybridization studies. Immunolabeling is completely abolished by preadsorption with synthetic rat 5-HT5A receptor (17-34). BlastP database sequence homology searches indicate that the amino acid sequence is unique to rat and mouse 5-HT5A receptor.

Photo Description: IHC image of neurons staining for the 5-HT5A receptor in the rat hippocampus (above) and the rat cortex (below). The tissue was fixed with 4% formaldehyde/0.05% glutaraldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:500 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.

5HT-5a cortex

Host: Rabbit

Quantity / Volume:  100 µL

State: Liquid

Reacts With: Clam, Rat

Availability: In Stock

Alternate Names:  5-hydroxytryptamine receptor 5A; 5-HT5A; REC17; 5ht5a,MR22; 5-hydroxytryptamine (serotonin) receptor 5A, G protein-coupled, anti-5-HT5a

RRID: AB_572213

Immunogen: Rat 5-HT5A receptor (17-34)

Gene Symbol / ID, Accession #:  Htr5a,25689 

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Publications

PROD # ANTIBODY CITATION SPECIES REACTIVITY APPL METHOD RESEARCH AREA # CITED BY PUBMED ID
24429 5-HT (Serotonin) 5A Receptor Antibody J Comp Neurol. 2004 Aug 30;476(4):316-29. 5-HT5A receptor localization in the rat spinal cord suggests a role in nociception and control of pelvic floor musculature. Doly S1, Fischer J, Brisorgueil MJ, Vergé D, Conrath M. Rat IHC 40 15282708
24429 5-HT (Serotonin) 5A Receptor Antibody Kreiling, Jill A., Raymond E. Stephens, and Carol L. Reinisch. "A mixture of environmental contaminants increases cAMP-dependent protein kinase in Spisula embryos." Environmental Toxicology and Pharmacology 19.1 (2005): 9-18. Clam IF, WB 7 21783457
24429 5-HT (Serotonin) 5A Receptor Antibody Oliver, K. R., et al. "Localization of 5-ht 5A receptor-like immunoreactivity in the rat brain." Brain research 867.1 (2000): 131-142. Rat IHC 75 10837806

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MSDS

This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.

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