The ImmunoStar 5-HT2C receptor antiserum was quality control tested using standard immunohistochemical methods. The antiserum demonstrates strongly positive labeling of rat choroid plexus and hippocampus using indirect immunofluorescent and biotin/avidin-HRP techniques. Recommended primary dilutions are /500 - 1/1000 in PBS - Bn/Av-HRP technique. Intensification methods such as nickel will approximately double the dilution factor as recommended.
The antibody was characterized by immunohistochemistry and Western blot. Western blotting revealed a single band of approximately 70 kD.
Preincubation of the antibody with an excess of the synthetic peptide blocked staining. Immunohistochemical staining of rat brain correlates well with Northern analysis, in situ hybridization and receptor autoradiography. BlastP database sequence homology searches confirmed that this sequence is unique to rat, mouse and human 5-HT2C receptors.
Photo Description: Gray scale IHC image of coronal rat brain section staining for the 5-HT2C receptor (above) and high magnification image of receptor expression in the cortex of the rat brain (below). The tissue was fixed with 4% formaldehyde/0.05% glutaraldehyde in 0.1 M phosphate buffer, before being removed and prepared for vibratome sectioning. Floating sections were incubated at RT in 10% goat serum in PBS, before standard IHC procedure. Primary antibody was incubated at 1:1000 for 48 hours, goat anti-rabbit secondary was subsequently added for 1 hour after washing with PBS. Light microscopy staining was achieved with standard biotin-streptavidin/HRP procedure and DAB chromogen.
Quantity / Volume: 100 µL
Reacts With: Mouse, Rat
Alternate Names: 5-hydroxytryptamine receptor 2C; 5-HT2C; 5HT-1C; 5-HTR2C; 5-hydroxytryptamine (serotonin) receptor 2C, G protein-coupled, anti-5-HT 2C
Immunogen: Rat 5-HT2C receptor (439-460)
Gene Symbol / ID, Accession #: Htr2c,25187
|PROD #||ANTIBODY||CITATION||SPECIES REACTIVITY||APPL METHOD||RESEARCH AREA||# CITED BY||PUBMED ID|
|24505||5-HT (Serotonin) 2C Receptor Antibody||Holmes, Gregory M. "5-Hydroxytryptamine< 2C< receptors on pudendal motoneurons innervating the external anal sphincter." Brain research 1057.1 (2005): 65-71.||Rat||6||16125683|
|24505||5-HT (Serotonin) 2C Receptor Antibody||Coulon P1, Kanyshkova T, Broicher T, Munsch T, Wettschureck N, Seidenbecher T, Meuth SG, Offermanns S, Pape HC, Budde T, Front Cell Neurosci. 2010 Oct 25;4:132. doi: 10.3389/fncel.2010.00132. eCollection 2010. "Activity Modes in Thalamocortical Relay Neurons are Modulated by G(q)/G(11) Family G-proteins - Serotonergic and Glutamatergic Signaling"||Mouse||IF||21267426|
|24505||5-HT (Serotonin) 2C Receptor Antibody||PLoS Genet. 2007 Jul;3(7):e124.Complex seizure disorder caused by Brunol4 deficiency in mice.Yang Y1, Mahaffey CL, Bérubé N, Maddatu TP, Cox GA, Frankel WN.||Mouse||WB||17677002|
|24505||5-HT (Serotonin) 2C Receptor Antibody||Zhang, Yu, et al. "Involvement of spinal serotonin receptors in electroacupuncture anti-hyperalgesia in an inflammatory pain rat model." Neurochemical research 36.10 (2011): 1785-1792.||Rat||IHC||13||21556842|
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This product contains the preservative sodium azide. The concentration percent of the sodium azide is ≤ .09%. Although this hazardous substance is a concentration below that required for the preparation of a Material Safety Data Sheet, we created a standard MSDS for your records.Download MSDS